Ida Velle Myking

• Bjerkan, Katrine Nergård; Alling, Renate; Myking, Ida Velle; Brysting, Anne Krag & Grini, Paul (2023). Genetic and environmental manipulation of Arabidopsis hybridization barriers uncovers antagonistic functions in endosperm cellularization. Frontiers in Plant Science. ISSN 1664-462X. 14, s. 1–16. doi: 10.3389/fpls.2023.1229060. Fulltekst i vitenarkiv

Se alle arbeider i Cristin

• Alling, Renate; Brysting, Anne Krag; Grini, Paul; Bjerkan, Katrine & Myking, Ida Velle (2022). Environmental and genetic factors affecting endosperm based post-zygotic hybridization barriers. Vis sammendrag

  Proper development of the triploid endosperm is vital for seed viability. During the early stages of development, the endosperm works as a nutrient sink, accumulating sugars from the surrounding tissues. During this developmental phase, endosperm division is devoid of cytokinesis, resulting in a syncytium. After sufficient accumulation of nutrients, the endosperm nuclei undergo cellularization, switching to serve as a nutrient source for the embryo. Tight regulation of cellularization is therefore vital for embryo development. Crosses between Arabidopsis thaliana as maternal contributor and A. arenosa as pollen donor cause late cellularization and reduced germination rate in hybrid seeds. Here we show the opposite cellularization phenotype in hybrid seeds when using A. lyrata as pollen donor also resulting in low germination rate. Stage specific endosperm reporters display ectopic expression in developing hybrid endosperm confirming disturbance of the source-sink endosperm phase change. We also observe accession genotype as well as single loci effects on the severity of the barrier. Interestingly, we found that temperature can alleviate this hybridization barrier in opposite directions depending on whether the hybrid seed exhibit early or late endosperm cellularization. We postulate that temperature dependency might be a general mechanism affecting endosperm-based hybridization barriers.

Se alle arbeider i Cristin