Emneord:
Biokjemi,
Molekylærbiologi
Publikasjoner
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Malecka, Agnieszka Maria; Østlie, Ingunn; Trøen, Gunhild; Malecki, Jedrzej Mieczyslaw; Delabie, Jan & Tierens, Anne
[Vis alle 9 forfattere av denne artikkelen]
(2023).
Gene expression analysis revealed downregulation of complement receptor 1 in clonal B cells in cold agglutinin disease .
Clinical and Experimental Immunology.
ISSN 0009-9104.
XX,
s. 1–10.
doi:
10.1093/cei/uxad135.
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Engelfriet, Melanie Lianne; Malecki, Jedrzej Mieczyslaw; Forsberg, Frida; Falnes, Pål & Ciosk, Rafal
(2023).
Characterization of the biochemical activity and tumor-promoting role of the dual protein methyltransferase METL-13/METTL13 in Caenorhabditis elegans.
PLOS ONE.
ISSN 1932-6203.
18(6),
s. 1–16.
doi:
10.1371/journal.pone.0287558.
Fulltekst i vitenarkiv
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Falnes, Pål; Malecki, Jedrzej Mieczyslaw; Herrera Gonzalez-Molina, Maria del Carmen; Bengtsen, Mads & Davydova, Erna
(2023).
Human seven-β-strand (METTL) methyltransferases - conquering the universe of protein lysine methylation.
Journal of Biological Chemistry.
ISSN 0021-9258.
299(6),
s. 1–15.
doi:
10.1016/j.jbc.2023.104661.
Fulltekst i vitenarkiv
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Malecki, Jedrzej Mieczyslaw; Willemen, Hanneke L.D.M.; Pinto, Rita; Ho, Angela Yeuan Yen; Moen, Anders & Eijkelkamp, Niels
[Vis alle 7 forfattere av denne artikkelen]
(2019).
Human FAM173A is a mitochondrial lysine-specific methyltransferase that targets adenine nucleotide translocase and affects mitochondrial respiration .
Journal of Biological Chemistry.
ISSN 0021-9258.
294(31),
s. 11654–11664.
doi:
10.1074/jbc.RA119.009045.
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Malecki, Jedrzej Mieczyslaw; Willemen, Hanneke L.D.M.; Pinto, Rita; Ho, Angela Yeuan Yen; Moen, Anders & Kjønstad, Ingrid Fadum
[Vis alle 10 forfattere av denne artikkelen]
(2018).
Lysine methylation by the mitochondrial methyltransferase FAM173B optimizes the function of mitochondrial ATP synthase.
Journal of Biological Chemistry.
ISSN 0021-9258.
294(4),
s. 1128–1141.
doi:
10.1074/jbc.RA118.005473.
Vis sammendrag
Lysine methylation is an important post-translational modification that is also present on mitochondrial proteins, but the mitochondrial lysine-specific methyltransferases (KMTs) responsible for modification are in most cases unknown. Here, we set out to determine the function of human family with sequence similarity 173 member B (FAM173B), a mitochondrial methyltransferase (MTase) reported to promote chronic pain. Using bioinformatics analyses and biochemical assays, we found that FAM173B contains an atypical, noncleavable mitochondrial targeting sequence responsible for its localization to mitochondria. Interestingly, CRISPR/Cas9-mediated KO of FAM173B in mammalian cells abrogated trimethylation of Lys-43 in ATP synthase c-subunit (ATPSc), a modification previously reported as ubiquitous among metazoans. ATPSc methylation was restored by complementing the KO cells with enzymatically active human FAM173B or with a putative FAM173B orthologue from the nematode Caenorhabditis elegans. Interestingly, lack of Lys-43 methylation caused aberrant incorporation of ATPSc into the ATP synthase complex and resulted in decreased ATP-generating ability of the complex, as well as decreased mitochondrial respiration. In summary, we have identified FAM173B as the long-sought KMT responsible for methylation of ATPSc, a key protein in cellular ATP production, and have demonstrated functional significance of ATPSc methylation. We suggest renaming FAM173B to ATPSc-KMT (gene name ATPSCKMT).
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Jakobsson, Magnus; Malecki, Jedrzej Mieczyslaw & Falnes, Pål Øystein
(2018).
Regulation of eukaryotic elongation factor 1 alpha (eEF1A) by dynamic lysine methylation.
RNA Biology.
ISSN 1547-6286.
15(3),
s. 314–319.
doi:
10.1080/15476286.2018.1440875.
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Malecka, Agnieszka Maria; Trøen, Gunhild; Tierens, Anne; Østlie, Ingunn; Malecki, Jedrzej Mieczyslaw & Randen, Ulla
[Vis alle 10 forfattere av denne artikkelen]
(2017).
Frequent somatic mutations of KMT2D (MLL2) and CARD11 genes in primary cold agglutinin disease.
British Journal of Haematology.
ISSN 0007-1048.
183(5),
s. 838–842.
doi:
10.1111/bjh.15063.
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Malecki, Jedrzej Mieczyslaw; Jakobsson, Magnus; Ho, Angela Yeuan Yen; Moen, Anders; Rustan, Arild & Falnes, Pål
(2017).
Uncovering human METTL12 as a mitochondrial methyltransferase that modulates citrate synthase activity through metabolite-sensitive lysine methylation.
Journal of Biological Chemistry.
ISSN 0021-9258.
292(43),
s. 17950–17962.
doi:
10.1074/jbc.M117.808451.
Fulltekst i vitenarkiv
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Jakobsson, Magnus; Malecki, Jedrzej Mieczyslaw; Nilges, Benedikt S.; Moen, Anders; Leidel, Sebastian A. & Falnes, Pål
(2017).
Methylation of human eukaryotic elongation factor
alpha (eEF1A) by a member of a novel protein lysine
methyltransferase family modulates mRNA translation.
Nucleic Acids Research (NAR).
ISSN 0305-1048.
45,
s. 8239–8254.
doi:
10.1093/nar/gkx432.
Fulltekst i vitenarkiv
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Malecki, Jedrzej Mieczyslaw; Aileni, Vinay Kumar; Ho, Angela Yeuan Yen; Schwarz, Juliane; Moen, Anders & Sørensen, Vigdis
[Vis alle 10 forfattere av denne artikkelen]
(2017).
The novel lysine specific methyltransferase METTL21B affects mRNA translation through inducible and dynamic methylation of Lys-165 in human eukaryotic elongation factor 1 alpha (eEF1A).
Nucleic Acids Research (NAR).
ISSN 0305-1048.
45(8),
s. 4370–4389.
doi:
10.1093/nar/gkx002.
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Malecki, Jedrzej Mieczyslaw; Dahl, Helge-Andre; Moen, Anders; Davydova, Erna & Falnes, Pål
(2016).
The METTL20 homologue from agrobacterium tumefaciens is a dual specificity protein-lysine methyltransferase that targets ribosomal protein L7/L12 and the β subunit of electron transfer flavoprotein (ETFβ).
Journal of Biological Chemistry.
ISSN 0021-9258.
291(18),
s. 9581–9595.
doi:
10.1074/jbc.M115.709261.
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Falnes, Pål; Jakobsson, Magnus; Davydova, Erna; Ho, Angela Yeuan Yen & Malecki, Jedrzej Mieczyslaw
(2016).
Protein lysine methylation by seven-β-strand methyltransferases.
Biochemical Journal.
ISSN 0264-6021.
473(14),
s. 1995–2009.
doi:
10.1042/BCJ20160117.
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Jakobsson, Magnus; Davydova, Erna; Malecki, Jedrzej Mieczyslaw; Moen, Anders & Falnes, Pål
(2015).
Saccharomyces cerevisiae eukaryotic elongation factor 1A (eEF1A) is methylated at Lys-390 by a METTL21-like methyltransferase.
PLOS ONE.
ISSN 1932-6203.
10(6).
doi:
10.1371/journal.pone.0131426.
Fulltekst i vitenarkiv
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Malecki, Jedrzej Mieczyslaw; Ho, Angela Yeuan Yen; Moen, Anders; Dahl, Helge-Andre & Falnes, Pål
(2015).
Human METTL20 is a mitochondrial lysine methyltransferase that targets the β subunit of electron transfer flavoprotein (ETFβ) and modulates its activity.
Journal of Biological Chemistry.
ISSN 0021-9258.
290(1),
s. 423–434.
doi:
10.1074/jbc.M114.614115.
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Davydova, Erna; Ho, Angela Yeuan Yen; Malecki, Jedrzej Mieczyslaw; Moen, Anders; Enserink, Jorrit & Jakobsson, Magnus
[Vis alle 8 forfattere av denne artikkelen]
(2014).
Identification and characterization of a novel evolutionarily conserved lysine-specific methyltransferase targeting eukaryotic translation elongation factor 2 (eEF2).
Journal of Biological Chemistry.
ISSN 0021-9258.
289(44),
s. 30499–30510.
doi:
10.1074/jbc.M114.601658.
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Haugsten, Ellen Margrethe; Malecki, Jedrzej M.; Bjørklund, Sunniva Maria Stordal; Olsnes, Sjur & Wesche, Jørgen
(2008).
Ubiquitination of fibroblast growth factor receptor 1 is required for its intracellular sorting but not for its endocytosis.
Molecular Biology of the Cell.
ISSN 1059-1524.
19,
s. 3390–3403.
doi:
10.1091/mbc.E07-12-1219.
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Wesche, Jørgen; Malecki, Jedrzej M.; Wiedlocha, Antoni; Skjerpen, Camilla Skiple; Claus, P & Olsnes, Sjur
(2006).
FGF-1 and FGF-2 require the cytosolic chaperone Hsp90 for translocation into the cytosol and the cell nucleus.
Journal of Biological Chemistry.
ISSN 0021-9258.
281,
s. 11405–11412.
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Wesche, Jørgen; Malecki, Jedrzej M.; Wiedlocha, Antoni; Ehsani, Maryam; Nilsen, Trine & Marcinkowska, Ewa
[Vis alle 7 forfattere av denne artikkelen]
(2005).
Two nuclear localization signals required for transport from the cytosol to the, nucleus of externally added FGF-1 translocated into cells.
Biochemistry (Moscow).
ISSN 0006-2979.
44.
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Malecki, Jedrzej M.; Wiedlocha, Antoni; Wesche, Jarlis & Olsnes, Sjur
(2002).
Vesicle transmembrane potential is required for translocation to cytosol externally added FGF-1.
EMBO Journal.
ISSN 0261-4189.
21,
s. 4480–4490.
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Malecka, Agnieszka Maria; Østlie, Ingunn; Trøen, Gunhild; Malecki, Jedrzej Mieczyslaw; Delabie, Jan & Tierens, Anne
[Vis alle 8 forfattere av denne artikkelen]
(2022).
Gene Expression Analysis Reveals Downregulation of Complement Receptor 1 in Cold Agglutinin Disease.
Blood.
ISSN 0006-4971.
140(1),
s. 6419–6420.
doi:
10.1182/blood-2022-156489.
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Malecka, Agnieszka Maria; Trøen, Gunhild; Delabie, Jan; Malecki, Jedrzej Mieczyslaw; Østlie, Ingunn & Tierens, Anne
[Vis alle 9 forfattere av denne artikkelen]
(2021).
The mutational landscape of cold agglutinin disease: CARD11 and CXCR4 mutations are correlated with lower hemoglobin levels.
American Journal of Hematology.
ISSN 0361-8609.
doi:
10.1002/ajh.26205.
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Malecka, Agnieszka Maria; Trøen, Gunhild; Tierens, Anne; Østlie, Ingunn; Malecki, Jedrzej Mieczyslaw & Randen, Ulla
[Vis alle 9 forfattere av denne artikkelen]
(2016).
Immunoglobulin heavy and light chain gene features are correlated with primary cold agglutinin disease onset and activity.
Haematologica.
ISSN 0390-6078.
101(9),
s. e361–e364.
doi:
10.3324/haematol.2016.146126.
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Publisert
25. jan. 2016 14:53
- Sist endret
14. feb. 2024 13:59