Rein Aasland

Utvalgte arbeider (se CRIStin eller ResearchGate for en mer komplett liste)

Dobrovolska O, Bril'kov M, Ødegård-Fougner Ø, Aasland R, Halskau Ø.  (2018) ¹H, ¹³C, and ¹⁵N resonance assignments of CW domain of the N-methyltransferase ASHH2 free and bound to the mono-, di- and tri-methylated histone H3 tail peptides. Biomol NMR Assign. 12:215-220.

Taxt, Arne Michael; Diaz, Yuleima; Aasland, Rein; Clements, John D.; Nataro, James Paul; Sommerfelt, Halvor; Puntervoll, Pål. (2016) Towards rational design of a toxoid vaccine against the heatstable toxin of Escherichia coli. Infection and Immunity 84:1239-1249

Rack JGM, Lutter T, Bjerga GEK, Guder C, Ehrhardt C, Varv S, Ziegler M, and Aasland R. (2014) The PHD finger of p300 influences its ability to acetylate histone and non-histone targets. Journal of Molecular Biology. 426:3960-3972

Rack JGM, Van Linden MR, Lutter T, Aasland R, and Ziegler M. 2014. Constitutive nuclear localization of an alternatively spliced sirtuin-2 isoform. Journal of Molecular Biology. 426:1677-1691.

Hoppmann V, Thorstensen T, Kristiansen PE, Veiseth SV, Rahman MA, Finne K, Aalen RB, and Aasland R. (2011) The CW domain, a new histone recognition module in chromatin proteins. EMBO J. 30: 1939-1952..

Xiao B, Sanders MJ, Underwood E, Heath R, Mayer FV, Carmena D, Jing C, Walker PA, Eccleston JF, Haire LF, Saiu P, Howell SA, Aasland R, Martin SR, Carling D, Gamblin SJ. (2011) Structure of mammalian AMPK and its regulation by ADP. Nature 472:230-233.

Denoeud F, Henriet S, Mungpakdee S, Aury JM, Da Silva C, Brinkmann H, Mikhaleva J, Olsen LC, Jubin C, Cañestro C, Bouquet JM, Danks G, Poulain J, Campsteijn C, Adamski M, Cross I, Yadetie F, Muffato M, Louis A, Butcher S, Tsagkogeorga G, Konrad A, Singh S, Jensen MF, Cong EH, Eikeseth-Otteraa H, Noel B, Anthouard V, Porcel BM, Kachouri-Lafond R, Nishino A, Ugolini M, Chourrout P, Nishida H, Aasland R, Huzurbazar S, Westhof E, Delsuc F, Lehrach H, Reinhardt R, Weissenbach J, Roy SW, Artiguenave F, Postlethwait JH, Manak JR, Thompson EM, Jaillon O, Du Pasquier L, Boudinot P, Liberles DA, Volff JN, Philippe H, Lenhard B, Roest Crollius H, Wincker P, and Chourrout D. (2010) Plasticity of animal genome architecture unmasked by rapid evolution of a pelagic tunicate. Science. 330:1381-5.

Justin N, De Marco V, Aasland R, and Gamblin SJ. (2010) Reading, writing and editing methylated lysines on histone tails: new insights from recent structural studies. Curr Opin Struct Biol. 20:730-8.

Taxt A, Aasland R, Sommerfelt H, Nataro J, and Puntervoll P. (2010) Heat-stable enterotoxin of enterotoxigenic Escherichia coli as a vaccine target. Infect Immun. 78:1824-31.

Gould CM, Diella F, Via A, Puntervoll P, Gemünd C, Chabanis-Davidson S, Michael S, Sayadi A, Bryne JC, Chica C, Seiler M, Davey NE, Haslam N, Weatheritt RJ, Budd A, Hughes T, Pas J, Rychlewski L, Travé G, Aasland R, Helmer-Citterich M, Linding R, and Gibson TJ. ELM: the status of the 2010 eukaryotic linear motif resource. Nucleic Acids Res. 2010 Jan;38(Database issue):D167-80.

Buchanan L, Durand-Dubief M, Roguev A, Sakalar C, Wilhelm B, Strålfors A, Shevchenko A, Aasland R, Shevchenko A, Ekwall K, and Francis Stewart A. (2009) The Schizosaccharomyces pombe JmjC-protein, Msc1, prevents H2A.Z localization in centromeric and subtelomeric chromatin domains. PLoS Genet. 5:e1000726

Thorstensen T, Grini PE, Mercy IS, Alm V, Erdal S, Aasland R, and Aalen RB. (2008) The Arabidopsis SET-domain protein ASHR3 is involved in stamen development and interacts with the bHLH transcription factor ABORTED MICROSPORES (AMS). Plant Mol Biol. 66:47-59

Glaser S, Schaft J, Lubitz S, Vintersten K, van der Hoeven F, Tufteland KR, Aasland R., Anastassiadis, K, Ang, SL., and Stewart, AF. (2006) Multiple epigenetic maintenance factors implicated by the loss of Mll2 in mouse development. Development.133:1423-1432.

Slagsvold T, Aasland R, Hirano S, Bache KG, Raiborg C, Trambaiolo D, Wakatsuki S, and Stenmark H. (2005) Eap45 in mammalian ESCRT-II binds ubiquitin via a phosphoinositide-interacting GLUE domain. J Biol Chem. 2005 280:19600-6.

Ragvin A, Valvatne H, Erdal S, Årskog V, Tufteland KR, Breen K, Øyan AM, Eberharter A, Gibson TJ, Becker PB, and Aasland R. (2004) Nucleosome binding by the bromodomain and PHD finger of the transcriptional cofactor p300. J Mol Biol. 337:773-88.

Korenjak M, Taylor-Harding B, Binne UK, Satterlee JS, Stevaux O, Aasland R, White-Cooper H, Dyson N, and Brehm A. (2004) Native E2F/RBF complexes contain Myb-interacting proteins and repress transcription of developmentally controlled E2F target genes. Cell. 119:181-93

Puntervoll P, Linding R, Gemund C, Chabanis-Davidson S, Mattingsdal M, Cameron S, Martin DM, Ausiello G, Brannetti B, Costantini A, Ferre F, Maselli V, Via A, Cesareni G, Diella F, Superti-Furga G, Wyrwicz L, Ramu C, McGuigan C, Gudavalli R, Letunic I, Bork P, Rychlewski L, Kuster B, Helmer-Citterich M, Hunter WN, Aasland R, and Gibson TJ. (2003) ELM server: A new resource for investigating short functional sites in modular eukaryotic proteins. Nucleic Acids Res. 31:3625-3630.

Roguev A, Schaft D, Shevchenko A, Aasland R, Shevchenko A, and Stewart AF. (2003) High conservation of the Set1/Rad6 axis of histone 3 lysine 4 methylation in budding and fission yeasts. J Biol Chem. 278:8487-8493.

Conklin D, Jonassen I, Aasland R, and Taylor WR. (2002) Association of nucleotide patterns with gene function classes: application to human 3' untranslated sequences. Bioinformatics. 18:182-189.

Pijnappel, W.W., Schaft D., Roguev, A., Schevchenko, A., Tekotte, H., Wilm, M., Rigaut, G., Seraphin B., Aasland, R., and Stewart, A.F. (2001) The S. cerevisiae SET3 complex includes two histone deacetylases, Hos2 and Hst1, and is a meiotic-specific repressor of the sporulation gene program. Genes Dev. 15:2991-3004

Roguev, A., Schaft, D., Schevchenko, A., Pijnappel, W.W., Wilm, M., Aasland, R., and Stewart, A.F. (2001) The Saccharomyces cerevisiae Set1 complex includes and Ash2 homologue and methylates histone 3 lysine 4. EMBO J. 20:137-148

Gaullier JM, Simonsen A, D'Arrigo A, Bremnes B, Aasland R., Stenmark H (1998) FYVE fingers bind PtdIns(3)P. Nature 394:432-3.

Gibson, T.J., Ramu, C. Christine Gemund, C., and Aasland, R. (1998). The APECED polyglandular autoimmune syndrome protein, AIRE-1, contains the SAND domain and is probably a transcription factor. Trends Biochem. Sci. 23:242-244

Aasland, R., Stewart, A. F., and Gibson, T. J. (1996). The SANT domain:A putative DNA binding domain in the SWI-SNF and ADA complexes, the transcriptional co-repressor N-CoR and TFIIIB. Trends Biochem. Sci. 21, 87-88.

Aasland, R., and Stewart, A. F. (1995). The chromo shadow domain, a second chromo domain in heterochromatin-binding protein 1, HP1. Nucl. Acids. Res. 23:3168-3173.

Aasland, R., Gibson, T. J., and Stewart, A. F. (1995). The PHD-finger: implications for chromatin-mediated transcriptional regulation. Trends Biochem. Sci. 20:56-59.

• Patalano, Fabiana; Sandve, Simen Rød; Aasland, Rein & Paulsen, Jonas (2025). TAD conservation in vertebrate genomes is driven by stabilising selection. BMC Biology. 23(1). doi: 10.1186/s12915-025-02362-0. Fulltekst i vitenarkiv Vis sammendrag

  Background Topologically associating domains (TADs) are fundamental structural and gene regulatory components of chromatin defined by regions of high intra-domain contact frequency. Though TADs are found across diverse metazoans, the extent of their evolutionary conservation is still debated. Results Here, we investigated the evolutionary conservation of TADs by analysing Hi-C data from 12 vertebrate species. We examined TAD numbers, borders, and gene positioning within TADs. We found that TAD features are all highly conserved across species, but decrease with evolutionary distance. Nevertheless, modelling TAD evolution using Ornstein–Uhlenbeck (OU) process revealed strong stabilising selection signatures for TAD number within the majority of syntenic blocks. These syntenic blocks under selection were enriched for highly conserved noncoding elements associated with developmental gene regulation (genomic regulatory blocks). However, strong signatures for stabilising selection for TAD numbers were also found independent of genomic regulatory blocks or genes with non-developmental functions. Conclusions These findings improve our understanding of TAD conservation and highlight stabilising selection as an important driver in 3D genome evolution. Although selection on TAD structures is pronounced for developmental genes, our findings highlight the importance of TADs in genome and organismal functions beyond developmental biology.

• Bril'kov, Maxim; Dobrovolska, Olena; Ødegård-Fougner, Øyvind; Turcu, Diana Cornelia; Strømland, Øyvind & Underhaug, Jarl [Vis alle 8 forfattere av denne artikkelen] (2022). Binding Specificity of ASHH2 CW Domain Toward H3K4me1 Ligand Is Coupled to Its Structural Stability Through Its α1-Helix. Frontiers in Molecular Biosciences. 9, s. 1–16. doi: 10.3389/fmolb.2022.763750. Fulltekst i vitenarkiv Vis sammendrag

  The CW domain binds to histone tail modifications found in different protein families involved in epigenetic regulation and chromatin remodeling. CW domains recognize the methylation state of the fourth lysine on histone 3 and could, therefore, be viewed as a reader of epigenetic information. The specificity toward different methylation states such as me1, me2, or me3 depends on the particular CW subtype. For example, the CW domain of ASHH2 methyltransferase binds preferentially to H3K4me1, and MORC3 binds to both H3K4me2 and me3 modifications, while ZCWPW1 is more specific to H3K4me3. The structural basis for these preferential bindings is not well understood, and recent research suggests that a more complete picture will emerge if dynamical and energetic assessments are included in the analysis of interactions. This study uses fold assessment by NMR in combination with mutagenesis, ITC affinity measurements, and thermal denaturation studies to investigate possible couplings between ASHH2 CW selectivity toward H3K4me1 and the stabilization of the domain and loops implicated in binding. The key elements of the binding site—the two tryptophans and the α1-helix form and maintain the binding pocket— were perturbed by mutagenesis and investigated. Results show that the α1-helix maintains the overall stability of the fold via the I915 and L919 residues and that the correct binding consolidates the loops designated as η1 and η3, as well as the C-terminal. This consolidation is incomplete for H3K4me3 binding to CW, which experiences a decrease in overall thermal stability on binding. Loop mutations not directly involved in the binding site, nonetheless, affect the equilibrium positions of the key residues.

• Dobrovolska, Olena; Brilkov, Maxim; Madeleine, Noëlly; Ødegaard-Fougner, Øyvind; Strømland, Øyvind & Martin, Stephen R. [Vis alle 15 forfattere av denne artikkelen] (2020). The Arabidopsis (ASHH2) CW domain binds monomethylated K4 of the histone H3 tail through conformational selection. The FEBS Journal. ISSN 1742-464X. 287(20), s. 4458–4480. doi: 10.1111/febs.15256. Fulltekst i vitenarkiv Vis sammendrag

  Chromatin post‐translational modifications are thought to be important for epigenetic effects on gene expression. Methylation of histone N‐terminal tail lysine residues constitutes one of many such modifications, executed by families of histone lysine methyltransferase (HKMTase). One such protein is ASHH2 from the flowering plant Arabidopsis thaliana, equipped with the interaction domain, CW, and the HKMTase domain, SET. The CW domain of ASHH2 is a selective binder of monomethylation at lysine 4 on histone H3 (H3K4me1) and likely helps the enzyme dock correctly onto chromatin sites. The study of CW and related interaction domains has so far been emphasizing lock–key models, missing important aspects of histone‐tail CW interactions. We here present an analysis of the ASHH2 CW‐H3K4me1 complex using NMR and molecular dynamics, as well as mutation and affinity studies of flexible coils. β‐augmentation and rearrangement of coils coincide with changes in the flexibility of the complex, in particular the η1, η3 and C‐terminal coils, but also in the β1 and β2 strands and the C‐terminal part of the ligand. Furthermore, we show that mutating residues with outlier dynamic behaviour affect the complex binding affinity despite these not being in direct contact with the ligand. Overall, the binding process is consistent with conformational selection. We propose that this binding mechanism presents an advantage when searching for the correct post‐translational modification state among the highly modified and flexible histone tails, and also that the binding shifts the catalytic SET domain towards the nucleosome. Databases Structural data are available in the PDB database under the accession code 6QXZ. Resonance assignments for CW42 in its apo‐ and holo‐forms are available in the BMRB database under the accession code 27251.

• Dobrovolska, Olena; Brilkov, Maxim; Ødegård‑Fougner, Øyvind; Aasland, Rein & Halskau, Øyvind (2018). 1H, 13C, and 15N resonance assignments of CW domain of the N-methyltransferase ASHH2 free and bound to the mono-, di- and tri-methylated histone H3 tail peptides. Biomolecular NMR Assignments. ISSN 1874-2718. 12(1), s. 215–220. doi: 10.1007/s12104-018-9811-x. Fulltekst i vitenarkiv
• Taxt, Arne Michael; Diaz, Yuleima; Aasland, Rein; Clements, John D.; Nataro, James Paul & Sommerfelt, Halvor [Vis alle 7 forfattere av denne artikkelen] (2016). Towards rational design of a toxoid vaccine against the heatstable toxin of Escherichia coli. Infection and Immunity. ISSN 0019-9567. 84(4), s. 1239–1249. doi: 10.1128/IAI.01225-15. Fulltekst i vitenarkiv
• Szilvay, Anne Marie; Sarria, Shirley Vanessa; Mannelqvist, Monica; Aasland, Rein & Furnes, Clemens (2016). Proteolytic activity assayed by subcellular localization switching of a substrate. Biochemistry and Biophysics Reports. 8, s. 23–28. doi: 10.1016/j.bbrep.2016.07.011. Fulltekst i vitenarkiv
• Taxt, Arne Michael; Diaz, Yuleima; Bacle, Amélie; Grauffel, Cédric; Reuter, Nathalie & Aasland, Rein [Vis alle 8 forfattere av denne artikkelen] (2014). Characterization of immunological cross-reactivity between enterotoxigenic Escherichia coli heat-stable toxin and human guanylin and uroguanylin. Infection and Immunity. ISSN 0019-9567. 82(7), s. 2913–2922. doi: 10.1128/IAI.01749-14. Fulltekst i vitenarkiv
• Rack, Johannes Gregor M; Linden, Magali R Van; Lutter, Timo; Aasland, Rein & Ziegler, Mathias (2014). Constitutive nuclear localization of an alternatively spliced sirtuin-2 isoform. Journal of Molecular Biology (JMB). ISSN 0022-2836. 426(8), s. 1677–1691. doi: 10.1016/j.jmb.2013.10.027. Fulltekst i vitenarkiv
• Rack, Johannes Gregor M; Lutter, Timo; Bjerga, Gro Elin Kjæreng; Guder, Corina; Ehrhardt, Christine & Varv, Signe [Vis alle 8 forfattere av denne artikkelen] (2014). The PHD finger of p300 influences its ability to acetylate histone and non-histone targets. Journal of Molecular Biology (JMB). ISSN 0022-2836. 426(24), s. 3960–3972. doi: 10.1016/j.jmb.2014.08.011. Fulltekst i vitenarkiv
• Hoppmann, Verena; Thorstensen, Tage; Kristiansen, Per Eugen; Veiseth, Silje Veie; Rahman, Mohummad Aminur & Finne, Kenneth [Vis alle 8 forfattere av denne artikkelen] (2011). The CW domain, a new histone recognition module in chromatin proteins. EMBO Journal. ISSN 0261-4189. 30(10), s. 1939–1952. doi: 10.1038/emboj.2011.108. Fulltekst i vitenarkiv
• Xiao, Bing; Sanders, Matthew J.; Underwood, Elizabeth; Heath, Richard; Mayer, Faith V. & Carmena, David [Vis alle 16 forfattere av denne artikkelen] (2011). Structure of mammalian AMPK and its regulation by ADP. Nature. ISSN 0028-0836. 472(7342), s. 230–233. doi: 10.1038/nature09932. Fulltekst i vitenarkiv
• Denoeud, France; Henriet, Simon; Mungpakdee, Sutada; Aury, Jean-Marc; Silva, Corinne Da & Brinkmann, Henner [Vis alle 56 forfattere av denne artikkelen] (2010). Plasticity of Animal Genome Architecture Unmasked by Rapid Evolution of a Pelagic Tunicate. Science. ISSN 0036-8075. 330(6009), s. 1381–1385. doi: 10.1126/science.1194167. Fulltekst i vitenarkiv Vis sammendrag

  Cong, Evelyne Huynh: Oppgir Sars i kilden, men har ikke norsk fødselsnr, og kan derfor ikke kreditere UiB./tøf

• Gould, Cathryn M.; Diella, Francesca; Via, Allegra; Puntervoll, Pål; Gemünd, Christine & Chabanis-Davidson, Sophie [Vis alle 23 forfattere av denne artikkelen] (2010). ELM: the status of the 2010 eukaryotic linear motif resource. Nucleic Acids Research (NAR). ISSN 0305-1048. 38, s. D167–D180. doi: 10.1093/nar/gkp1016. Fulltekst i vitenarkiv Vis sammendrag

  Linear motifs are short segments of multidomain proteins that provide regulatory functions independently of protein tertiary structure. Much of intracellular signalling passes through protein modifications at linear motifs. Many thousands of linear motif instances, most notably phosphorylation sites, have now been reported. Although clearly very abundant, linear motifs are difficult to predict de novo in protein sequences due to the difficulty of obtaining robust statistical assessments. The ELM resource at http://elm.eu.org/ provides an expanding knowledge base, currently covering 146 known motifs, with annotation that includes >1300 experimentally reported instances. ELM is also an exploratory tool for suggesting new candidates of known linear motifs in proteins of interest. Information about protein domains, protein structure and native disorder, cellular and taxonomic contexts is used to reduce or deprecate false positive matches. Results are graphically displayed in a ‘Bar Code’ format, which also displays known instances from homologous proteins through a novel ‘Instance Mapper’ protocol based on PHI-BLAST. ELM server output provides links to the ELM annotation as well as to a number of remote resources. Using the links, researchers can explore the motifs, proteins, complex structures and associated literature to evaluate whether candidate motifs might be worth experimental investigation.

• Justin, Neil; Marco, Valeria De; Aasland, Rein & Gamblin, Steven J. (2010). Reading, writing and editing methylated lysines on histone tails: new insights from recent structural studies. Current Opinion in Structural Biology. ISSN 0959-440X. 20(6), s. 730–738. doi: 10.1016/j.sbi.2010.09.012. Fulltekst i vitenarkiv
• Taxt, Arne Michael; Aasland, Rein; Sommerfelt, Halvor; Nataro, James & Puntervoll, Pål (2010). Heat-stable enterotoxin of enterotoxigenic escherichia coli as a vaccine target. Infection and Immunity. ISSN 0019-9567. 78(5), s. 1824–1831. doi: 10.1128/IAI.01397-09. Fulltekst i vitenarkiv
• Buchanan, Luke; Durand-Dubief, Mickaël; Roguev, Assen; Cagri, Sakalar; Wilhelm, Brian & Stålfors, Annelie [Vis alle 11 forfattere av denne artikkelen] (2009). The Schizosaccharomyces pombe JmjC-protein, Msc1, prevents H2A.Z localization in centromeric and subtelomeric chromatin domains. PLoS Genetics. ISSN 1553-7390. 5(11). doi: 10.1371/journal.pgen.1000726. Fulltekst i vitenarkiv Vis sammendrag

  Eukaryotic genomes are repetitively packaged into chromatin by nucleosomes, however they are regulated by the differences between nucleosomes, which establish various chromatin states. Local chromatin cues direct the inheritance and propagation of chromatin status via self-reinforcing epigenetic mechanisms. Replication-independent histone exchange could potentially perturb chromatin status if histone exchange chaperones, such as Swr1C, loaded histone variants into wrong sites. Here we show that in Schizosaccharomyces pombe, like Saccharomyces cerevisiae, Swr1C is required for loading H2A.Z into specific sites, including the promoters of lowly expressed genes. However S. pombe Swr1C has an extra subunit, Msc1, which is a JumonjiC-domain protein of the Lid/Jarid1 family. Deletion of Msc1 did not disrupt the S. pombe Swr1C or its ability to bind and load H2A.Z into euchromatin, however H2A.Z was ectopically found in the inner centromere and in subtelomeric chromatin. Normally this subtelomeric region not only lacks H2A.Z but also shows uniformly lower levels of H3K4me2, H4K5, and K12 acetylation than euchromatin and disproportionately contains the most lowly expressed genes during vegetative growth, including many meiotic-specific genes. Genes within and adjacent to subtelomeric chromatin become overexpressed in the absence of either Msc1, Swr1, or paradoxically H2A.Z itself. We also show that H2A.Z is N-terminally acetylated before, and lysine acetylated after, loading into chromatin and that it physically associates with the Nap1 histone chaperone. However, we find a negative correlation between the genomic distributions of H2A.Z and Nap1/Hrp1/Hrp3, suggesting that the Nap1 chaperones remove H2A.Z from chromatin. These data describe H2A.Z action in S. pombe and identify a new mode of chromatin surveillance and maintenance based on negative regulation of histone variant misincorporation.

• Thorstensen, Tage; Grini, Paul E.; Mercy, Inderjit S.; Alm, Vibeke; Erdal, Sigrid & Aasland, Rein [Vis alle 7 forfattere av denne artikkelen] (2008). The Arabidopsis SET-domain protein ASHR3 is involved in stamen development and interacts with the bHLH transcription factor ABORTED MICROSPORES (AMS). Plant Molecular Biology. ISSN 0167-4412. 66(1), s. 47–59. doi: 10.1007/s11103-007-9251-y. Fulltekst i vitenarkiv
• Pánek, Josef; Eidhammer, Ingvar & Aasland, Rein (2007). Using hydropathy features for function prediction of membrane proteins. Molecular membrane biology. ISSN 0968-7688. 24(4), s. 304–312. doi: 10.1080/09687860701213884. Fulltekst i vitenarkiv
• Glaser, Stefan; Schaft, Julia; Lubitz, Sandra; Vintersten, Kristina; Hoeven, Frank van der & Tufteland, Katharina Reksten [Vis alle 10 forfattere av denne artikkelen] (2006). Multiple epigenetic maintenance factors implicated by the loss of Mll2 in mouse development. Development. ISSN 0950-1991. 133(8), s. 1423–1432. doi: 10.1242/dev.02302. Fulltekst i vitenarkiv
• Panek, Josef; Eidhammer, Ingvar & Aasland, Rein (2005). A New Method for Identification of Protein (Sub)Families in a Set of Proteins Based on Hydropathy Distribution in Proteins. Proteins: Structure, Function, and Bioinformatics. ISSN 0887-3585. 58, s. 923–934. Fulltekst i vitenarkiv

Se alle arbeider i NVA

• Gundersen, Sveinung; Faci, Pavel Vazquez; Bianchini, Federico; Baskaran, Joshua; Cheneby, Jeanne Marie & Hovig, Eivind [Vis alle 7 forfattere av denne artikkelen] (2026). fairtracks/omnipy: Omnipy v0.23.1. doi: 10.5281/ZENODO.21999005. Fulltekst i vitenarkiv
• Goksøyr, Anders; Delsett, Lene Liebe & Aasland, Rein (2025). På sporet av moderne biologi i Museets samlinger. I Aslaksen, Knut Olav; Tandberg, Anne Helene S.; Rio, Knut; Wrigglesworth, Melanie; Fossen, Haakon & Årrestad, Kari Klæboe (Red.), Årbok for Universitetsmuseet 2025: "200 år". Universitetsmuseet i Bergen. ISSN 9788278870617. s. 103–119. Fulltekst i vitenarkiv Vis sammendrag

  Naturhistorisk museum på Nygårdshøyden er et staselig murbygg som representerer 200 år med samlinger, forskning og formidling. Bak den høye eikedøren finner vi et moderne museum der mye av historien fortsatt er bevart i form av restaurerte glassmontre, preparater på glass og rengjorte hvalskjeletter i den majestetiske Hvalsalen. Men ikke alt av 200 års museumsinnsamling får plass i selve museet. Mye er lagret i museets magasiner. Vi reflekterer over samlingenes betydning gjennom 200 år til i dag og forbindelsene til moderne biologi, samt hvordan ny teknologi belyser gamle og nye spørsmål.

• Helland, Dag Emil; Haarr, Lars & Aasland, Rein (2025). Nekrolog: Ruth Kleppe Aakvaag. Aftenposten (morgenutg. : trykt utg.). ISSN 0804-3116. Fulltekst i vitenarkiv
• Aasland, Rein (2024). Coronavirusets opprinnelse. [TV]. NRK TV Dagsnytt 18. Fulltekst i vitenarkiv
• Aasland, Rein & Klein, Jörn (2024). Naturlig opphav til sars-cov-2 er fortsatt mest sannsynlig. Fulltekst i vitenarkiv
• Jemterud, Torkild; Ørmen, Torfinn & Aasland, Rein (2022). Abels kjeller - Neanderthalerne og meg - Om Nobelprisen til Svante Pääbo. [Radio]. https://radio.nrk.no/podkast/abels_taarn/l_87aef21d-8c0d-4d1. Fulltekst i vitenarkiv Vis sammendrag

  Svante Pääbo har blitt tildelt årets Nobelpris i medisin eller fysiologi - for sin kartlegning av Neanderthalernes og Denisovarenes DNA. Mye av dette arvestoffet finner vi igjen i moderne mennesker over store deler av kloden. Hvordan klarer man å hente ut DNA fra så gamle knokler, og hva kan denne kunnskapen fortelle oss om hva det vil si å være et menneske. Torkild Jemterud (NRK) i samtale med Torfinn Ørmen og Rein Aasland.

• Brilkov, Maxim; Dobrovolska, Olena; Aasland, Rein & Halskau, Øyvind (2018). CW-domain of ASHH2 methyltransferase: structural basis of ligand binding and specificity. Fulltekst i vitenarkiv
• Furnes, Clemens; Mannelqvist, Monica; Sarria, Shirley Vanessa; Aasland, Rein & Szilvay, Anne Marie (2016). In vivo proteolytic activity assayed by subcellular localization switching. Fulltekst i vitenarkiv
• Aasland, Rein (2012). Histone Recognition Modules: More than just passive recognition. Fulltekst i vitenarkiv
• Aasland, Rein (2012). Kromatin, epigenetikk og cellenes hukommelse. Fulltekst i vitenarkiv
• Vollsund, Pernille; Bergholtz, Stine Louise; Sæther, Thomas; Aasland, Rein & Gabrielsen, Odd Stokke (2009). Understanding the phosphorylation-sumoylation cross talk in the transcription factor c-Myb. Fulltekst i vitenarkiv Vis sammendrag

  The transcription factor c-Myb is involved in proliferation and differentiation of hematopoietic cells. c-Myb is regulated by several post-translational modifications (PTMs), including phosphorylation and sumoylation. SUMO (small ubiquitin related modifier) modifies the target protein by covalent conjugation to specific lysines. Previous studies from our group described sumoylation of c-Myb at two sites (K503 and K527) in its negative regulatory domain (NRD) causing severely reduced activity1 . SUMO-conjugation mainly modulates the ability of c-Myb to synergize on complex promoters2. Various phosphorylations of c-Myb have also been reported3,4. Preliminary results indicated that sumoylation and phosphorylation might be closely linked, suggesting a PTM crosstalk. Hence, a series of mutants were made to establish if there is a dependency between these modifications or if they are independent. The preliminary data reported here are mainly based on analysis of migration shifts after phosphatase treatments.

• Aasland, Rein (2008). Small Domains and Short Motifs in Chromatin-Based Gene Regulation. Fulltekst i vitenarkiv
• Aasland, Rein (2007). Small domains and short linear motifs involved in chromatin-based gene regulation. Fulltekst i vitenarkiv
• Aasland, Rein (2006). Small Domains and Short Motifs in Transcriptional Regulation. Fulltekst i vitenarkiv
• Aasland, Rein (2006). Small domains and short linear motifs in epigenetic gene regulation. Fulltekst i vitenarkiv
• Aasland, Rein (2006). Bioinformatics of functional sites: short linear motifs involved in protein interaction and post-translational modification. Fulltekst i vitenarkiv
• Aasland, Rein (2006). Opportunities for collaboration between EMBL and Norwegian universities. Fulltekst i vitenarkiv
• Aasland, Rein (2005). Bioinformatikk. Fulltekst i vitenarkiv
• Ertsås, Henriette Christie; Lorens, James; LaBarge, Mark A. & Aasland, Rein (2018). The effects of ageing on microenvironment-contextual epithelial cell signalling. Universitetet i Bergen. ISSN 9788230838044. Fulltekst i vitenarkiv Vis sammendrag

  Age is the greatest risk factor for developing cancer. Two-thirds of cancer diagnoses occur in people over the age of 65. This increased vulnerability to tumourigenesis is attributed to intrinsic cellular changes, in particular the agerelated telomere shortening and the accumulation of mutations over time and. We propose that extrinsic factors comprising age-related alterations in the tissue microenvironment are also important in cancer development. We sought to elucidate how the microenvironmental affects cells, and how this is related to cancer development. This knowledge can be utilized to improve prevention, diagnosis and treatment of cancer in our ageing population. Cellular function is coordinated by microenvironmental factors such as growth factors, cytokines as well as extracellular matrix proteins. Soluble factormediated signal transduction is strongly influenced by microenvironmental context. To allow single cell level measurement of the microenvironmental contextual effect on cell signaling, we developed a novel flow cytometry method: microsphere cytometry. Single normal or neoplastic cells were adhered to uniform microspheres that display mimetic-microenvironments comprising surface combinations of extracellular matrix (ECM) in the presence of soluble agonists or antagonists. Temporal signaling responses were measured with fluorophore-conjugated antibodies that recognize responsedependent epitopes by multiparametric flow cytometry. Using this approach, we demonstrated that microenvironment-mimetic combinations of growth factors and extracellular matrix proteins generate distinct cellular signaling signatures from normal and patient biopsy-derived neoplastic cells. We asked whether the ageing process affects how human mammary epithelial cells (HMEC) respond to microenvironmental signals, and if this altered response results in increased susceptibility to oncogenic transformation. Using microsphere cytometry we analyzed age-dependent changes in human mammary myoepithelial and luminal epithelial cells exposed to different ECM and growth factors. We found that ECM–mediated MAP kinase and PI3K pathway activation levels in HMEC are attenuated with age, and that the diminished signaling magnitude in HMEC from ageing women correlated with reduced probability of activating oncogene-induced senescence. Our results suggest that attenuated cell signaling response may reduce the likelihood of activating oncogene induced senescence, for cells in ageing women. We hypothesize this is the result of age-related changes to the microenvironment that support age-emergent cellular phenotypes with increased cancer susceptibility.

• Gavgani, Fatemeh Mazloumi; Lewis, Aurelia Eva & Aasland, Rein (2017). Nucleolar roles of the PI3K pathway in cancer and differentiation. Universitetet i Bergen. ISSN 9788230838662. Fulltekst i vitenarkiv Vis sammendrag

  The phosphoinositide 3-kinase (PI3K) pathway is essential for many cellular functions including; proliferation, cell survival, differentiation and metabolism. Although this pathway has been well studied in the cytoplasm, the role of PI3Ks in the nucleus has also been emerging as essential determinants of cell function. Previous work from our group has mapped the class I PI3K p110β and its product PtdIns(3,4,5)P3 to the nucleus and nucleolus in a breast cancer cell line. Here, we aimed to understand and characterize the role of this kinase and its lipid product in the nucleus during tumorigenesis and adipocyte differentiation. We first found that the PI3K is active in the nucleus of differentiating 3T3-L1 and endometrial cancer cells and identified an active form of Akt in the nucleoli of endometrial cancer cell lines. Using subcellular fractionation, mass spectrometry and different staining methods, we confirmed the nucleolar localization of PtdIns(3,4,5)P3 in several cell types but at different levels. In adipocytes, the levels of p110β were low in the nucleus and nucleoli upon differentiation. In contrast, endometrial cancer cell lines had high amounts of nuclear p110β and PtdIns(3,4,5)P3. The nucleolus is the sub nuclear site where ribosomal RNA (rRNA) transcription and processing occurs and interestingly we found that inhibition of p110β reduced the level of transcription of this RNA species. In addition, endometrial cancer cell lines with high amounts of nuclear p110β and PtdIns(3,4,5)P3 showed significant elevations in pre-rRNA transcription. To gain further insights of the nuclear roles of PtdIns(3,4,5)P3, we used a quantitative interactomics approach and identified 219 PtdIns(3,4,5)P3 interacting proteins that were highly enriched in cytokinesis, RNA processing and DNA repair functions. PARP1 (Poly (ADP-ribose) polymerase 1), a DNA repair protein abundant in the nucleoli, was one of the identified proteins which was then validated to interact with phosphoinositides directly, including PtdIns(3,4,5)P3. The nucleolar presence and co-localization of PARP1 with PtdIns(3,4,5)P3 was dependent on active rRNA transcription. Altogether the results from this thesis suggests an active role of the class I PI3K p110β and its product PtdIns(3,4,5)P3 in the nucleolus of differentiating adipocytes and endometrial cancer cells but with distinct regulation.

• Strømland, Øyvind & Aasland, Rein (2012). Specificity of CW domains for modified histone peptides. Universitetet i Bergen. Fulltekst i vitenarkiv
• Lycke, Kirsten Hofgaard; Eriksen, Harald M.; Hanken, Ingrid Maria; Granaas, Rakel Christina; Aasland, Rein & Løfgren, Trond (2009). Utredning om etablering av Sentre for fremragende utdanning (SFU). Innstilling fra Universitets- og høgskolerådet til Kunnskapsdepartemtentet. 15. juni 2009. Universitets- og høgskolerådete. Fulltekst i vitenarkiv
• Bjerga, Gro Elin Kjæreng & Aasland, Rein (2009). The functional duality of p300: The acetyl-lysine binding domains communicate with the acetyltransferase domain. Universitetet i Bergen. ISSN 9788230807590. Fulltekst i vitenarkiv Vis sammendrag

  PhD-avhandling

• Tellgren, Åsa; Liberles, David & Aasland, Rein (2007). A study of adaptive evolution in mammals: Myostatin in Artiodactyls Plasminogen activator in vampire bats. Universitetet i Bergen. Fulltekst i vitenarkiv
• Bielen, Holger; Technau, Ulrich & Aasland, Rein (2007). Tracing the evolution of the mesoderm - characterisation of Brachyury paralogs in a diploblastic animal, the cnidarian Hydra. Universitetet i Bergen. Fulltekst i vitenarkiv
• Tufteland, Katharina Reksten & Aasland, Rein (2007). Function prediction and annotation of protein domains. Universitetet i Bergen. Fulltekst i vitenarkiv
• Rønneberg, Natalia Godø & Aasland, Rein (2007). An investigation into the interaction between the PHD finger and bromodomain of p300 and nucleosomes. Universitetet i Bergen. Fulltekst i vitenarkiv
• Fernandes, Ana Clara & Aasland, Rein (2006). Sumoylation of RanGap1 motif in c-Myb context. Universitetet i Bergen. Fulltekst i vitenarkiv
• Ragvin, Anja; Aasland, Rein & Andersen, Øyvind M (2005). The PHD finger: a nucleosome-binding protein module involved in epigenetic gene regulation. Universitetet i Bergen. ISSN 9788276530308. Fulltekst i vitenarkiv
• Furnes, Clemens; Szilvay, Anne Marie & Aasland, Rein (2005). Subcellular localization, oligomerization and trafficking of HIV-1 Rev. Universitetet i Bergen. ISSN 9788230800201. Fulltekst i vitenarkiv

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