Emneord:
Cellebiologi
Publikasjoner
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Stonyte, Vilte; Martîn, Ruth Martîn; Segura-Pena, Dario; Sekulic, Nikolina & Lopez Aviles, Sandra
(2020).
Requirement of PP2A-B56 Par1 for the Stabilization of the CDK Inhibitor Rum1 and Activation of APC/C Ste9 during Pre-Start G1 in S. pombe.
iScience.
ISSN 2589-0042.
23(5).
doi:
10.1016/j.isci.2020.101063.
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Exit from the cell cycle during the establishment of quiescence and upon cell differentiation requires the sustained inactivation of CDK complexes. Fission yeast cells deprived of nitrogen halt cell cycle progression in pre-Start G1, before becoming quiescent or undergoing sexual differentiation. The CDK inhibitor Rum1 and the APC/C activator Ste9 are fundamental for this arrest, but both are down-regulated by CDK complexes. Here, we show that PP2A-B56Par1 is instrumental for Rum1 stabilization and Ste9 activation. In the absence of PP2A-B56Par1, cells fail to accumulate Rum1, and this results in persistent CDK activity, Ste9 inactivation, retention of the mitotic cyclin Cdc13, and impaired withdrawal from the cell cycle during nitrogen starvation. Importantly, mutation of a putative B56 interacting motif in Rum1 recapitulates these defects. These results underscore the relevance of CDK-counteracting phosphatases in cell differentiation, establishment of the quiescent state, and escape from it in cancer cells.
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Martîn, Ruth Martîn; Stonyte, Vilte & Lopez Aviles, Sandra
(2020).
Protein phosphatases in G1 regulation.
International Journal of Molecular Sciences.
ISSN 1661-6596.
21:395(2),
s. 1–15.
doi:
10.3390/ijms21020395.
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Eukaryotic cells make the decision to proliferate, to differentiate or to cease dividing during G1, before passage through the restriction point or Start. Keeping cyclin-dependent kinase (CDK) activity low during this period restricts commitment to a new cell cycle and is essential to provide the adequate timeframe for the sensing of environmental signals. Here, we review the role of protein phosphatases in the modulation of CDK activity and as the counteracting force for CDK-dependent substrate phosphorylation, in budding and fission yeast. Moreover, we discuss recent findings that place protein phosphatases in the interface between nutritional signalling pathways and the cell cycle machinery.
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Rothe, Christiane; Rødland, Gro Elise; Anda, Silje; Stonyte, Vilte; Boye, Erik & Lopez-Aviles, Sandra
[Vis alle 7 forfattere av denne artikkelen]
(2017).
A checkpoint-independent mechanism delays entry into mitosis after UV irradiation.
Journal of Cell Science.
ISSN 0021-9533.
130(23),
s. 4028–4037.
doi:
10.1242/jcs.204693.
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Martîn, Ruth Martîn; Portantier, Marina; Chica-Balaguera, Nathalia; Nyquist-Andersen, Mari; Mata, J & Lopez-Aviles, Sandra
(2017).
A PP2A-B55-Mediated Crosstalk between TORC1
and TORC2 Regulates the Differentiation Response
in Fission Yeast.
Current Biology.
ISSN 0960-9822.
27(2),
s. 175–188.
doi:
10.1016/j.cub.2016.11.037.
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Gómez-Hierro, Alba; Lambea, Eva; Giménez-Zaragoza, David; Lopez Aviles, Sandra; Yance-Chávez, Tula & Montserrat, Marta
[Vis alle 9 forfattere av denne artikkelen]
(2015).
Ssp1 CaMKK: A sensor of actin polarization that controls mitotic commitment through Srk1 in schizosaccharomyces pombe.
PLOS ONE.
ISSN 1932-6203.
10(11).
doi:
10.1371/journal.pone.0143037.
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Publisert
27. okt. 2022 15:42
- Sist endret
27. okt. 2022 15:42