vildeol

• Lalun, Vilde Olsson & Butenko, Melinka Alonso (2025). Plant Peptide Ligands as Temporal and Spatial Regulators. Annual Review of Plant Biology. ISSN 1543-5008. 76(1), s. 229–253. doi: 10.1146/annurev-arplant-070324-041348. Fulltekst i vitenarkiv Vis sammendrag

  Throughout the life cycle of a plant, numerous responses need to be carefully regulated to ensure proper development and appropriate responses to external stimuli, and plant hormones play a crucial role in this regulation. Since the early 1990s, there has been expansive research elucidating the central role that peptide ligands play as intrinsic short- and long-distance communicators during development and as regulators of phenotypic plasticity. In this review, we focus on recently discovered mechanisms that ensure correct spatial and temporal cellular responses triggered by peptide ligands and provide examples of how peptide processing proteins and apoplastic conditions can regulate peptide activity in a timely manner.

• Rzemieniewski, Jakub; Leicher, Henriette; Lee, Hyun Kyung; Broyart, Caroline; Nayem, Shahran & Wiese, Christian [Vis alle 14 forfattere av denne artikkelen] (2024). CEP signaling coordinates plant immunity with nitrogen status. Nature Communications. 15(1). doi: 10.1038/s41467-024-55194-x. Fulltekst i vitenarkiv Vis sammendrag

  Plant endogenous signaling peptides shape growth, development and adaptations to biotic and abiotic stress. Here, we identify C-TERMINALLY ENCODED PEPTIDEs (CEPs) as immune-modulatory phytocytokines in Arabidopsis thaliana. Our data reveals that CEPs induce immune outputs and are required to mount resistance against the leaf-infecting bacterial pathogen Pseudomonas syringae pv. tomato. We show that effective immunity requires CEP perception by tissue-specific CEP RECEPTOR 1 (CEPR1) and CEPR2. Moreover, we identify the related RECEPTOR-LIKE KINASE 7 (RLK7) as a CEP4-specific CEP receptor contributing to CEP-mediated immunity, suggesting a complex interplay of multiple CEP ligands and receptors in different tissues during biotic stress. CEPs have a known role in the regulation of root growth and systemic nitrogen (N)-demand signaling. We provide evidence that CEPs and their receptors promote immunity in an N status-dependent manner, suggesting a previously unknown molecular crosstalk between plant nutrition and cell surface immunity. We propose that CEPs and their receptors are central regulators for the adaptation of biotic stress responses to plant-available resources.

• Ma, Xingshuai; He, Zidi; Yuan, Ye; Liang, Zhijian; Zhang, Hang & Lalun, Vilde Olsson [Vis alle 12 forfattere av denne artikkelen] (2024). The transcriptional control of LcIDL1–LcHSL2 complex by LcARF5 integrates auxin and ethylene signaling for litchi fruitlet abscission. Journal of Integrative Plant Biology (JIPB). ISSN 1672-9072. 66(6). doi: 10.1111/jipb.13646. Fulltekst i vitenarkiv Vis sammendrag

  At the physiological level, the interplay between auxin and ethylene has long been recognized as crucial for the regulation of organ abscission in plants. However, the underlying molecular mechanisms remain unknown. Here, we identified transcription factors involved in indoleacetic acid (IAA) and ethylene (ET) signaling that directly regulate the expression of INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) and its receptor HAESA (HAE), which are key components initiating abscission. Specifically, litchi IDA-like 1 (LcIDL1) interacts with the receptor HAESA-like 2 (LcHSL2). Through in vitro and in vivo experiments, we determined that the auxin response factor LcARF5 directly binds and activates both LcIDL1 and LcHSL2. Furthermore, we found that the ETHYLENE INSENSITIVE 3-like transcription factor LcEIL3 directly binds and activates LcIDL1. The expression of IDA and HSL2 homologs was enhanced in LcARF5 and LcEIL3 transgenic Arabidopsis plants, but reduced in ein3 eil1 mutants. Consistently, the expressions of LcIDL1 and LcHSL2 were significantly decreased in LcARF5- and LcEIL3-silenced fruitlet abscission zones (FAZ), which correlated with a lower rate of fruitlet abscission. Depletion of auxin led to an increase in 1-aminocyclopropane-1-carboxylic acid (the precursor of ethylene) levels in the litchi FAZ, followed by abscission activation. Throughout this process, LcARF5 and LcEIL3 were induced in the FAZ. Collectively, our findings suggest that the molecular interactions between litchi AUXIN RESPONSE FACTOR 5 (LcARF5)–LcIDL1/LcHSL2 and LcEIL3–LcIDL1 signaling modules play a role in regulating fruitlet abscission in litchi and provide a long-sought mechanistic explanation for how the interplay between auxin and ethylene is translated into the molecular events that initiate abscission.

• Reinar, William Brynildsen; Krabberød, Anders Kristian; Lalun, Vilde Olsson; Butenko, Melinka Alonso & Jakobsen, Kjetill Sigurd (2024). Short tandem repeats delineate gene bodies across eukaryotes. Nature Communications. 15(1), s. 1–13. doi: 10.1038/s41467-024-55276-w. Fulltekst i vitenarkiv Vis sammendrag

  Short tandem repeats (STRs) have emerged as important and hypermutable sites where genetic variation correlates with gene expression in plant and animal systems. Recently, it has been shown that a broad range of transcription factors (TFs) are affected by STRs near or in the DNA target binding site. Despite this, the distribution of STR motif repetitiveness in eukaryote genomes is still largely unknown. Here, we identify monomer and dimer STR motif repetitiveness in 5.1 billion 10-bp windows upstream of translation starts and downstream of translation stops in 25 million genes spanning 1270 species across the eukaryotic Tree of Life. We report that all surveyed genomes have gene-proximal shifts in motif repetitiveness. Within genomes, variation in gene-proximal repetitiveness landscapes correlated to the function of genes; genes with housekeeping functions were depleted in upstream and downstream repetitiveness. Furthermore, the repetitiveness landscapes correlated with TF binding sites, indicating that gene function has evolved in conjunction with cis-regulatory STRs and TFs that recognize repetitive sites. These results suggest that the hypermutability inherent to STRs is canalized along the genome sequence and contributes to regulatory and eco-evolutionary dynamics in all eukaryotes.

• Lalun, Vilde Olsson; Breiden, Maike; Trigo, Sergio Galindo; Smakowska-Luzan, Elwira; Simon, Rüdiger & Butenko, Melinka Alonso (2023). A dual function of the IDA peptide in regulating cell separation and modulating plant immunity at the molecular level. eLIFE. 12, s. 1–37. doi: 10.7554/eLife.87912.1. Fulltekst i vitenarkiv Vis sammendrag

  The abscission of floral organs and emergence of lateral roots in Arabidopsis is regulated by the peptide ligand INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) and the receptor protein kinases HAESA (HAE) and HAESA-LIKE 2 (HSL2). During these cell separation processes, the plant induces defense-associated genes to protect against pathogen invasion. However, the molecular coordination between abscission and immunity has not been thoroughly explored. Here we show that IDA induces a receptor-dependent release of cytosolic calcium ions (Ca2+) and apoplastic production of reactive oxygen species, which are signatures of early defense responses. In addition, we find that IDA promotes late defense responses by the transcriptional upregulation of genes known to be involved in immunity. When comparing the IDA induced early immune responses to known immune responses, such as those elicited by flagellin22 treatment, we observe both similarities and differences. We propose a molecular mechanism by which IDA promotes signatures of an immune response in cells destined for separation to guard them from pathogen attack.

• Breiden, Maike; Olsson, Vilde; Blümke, Patrick; Schlegel, Jenia; Gustavo-Pinto, Karine & Dietrich, Petra [Vis alle 8 forfattere av denne artikkelen] (2021). The Cell Fate Controlling CLE40 Peptide Requires CNGCs to Trigger Highly Localized Ca2+ Transients in Arabidopsis thaliana Root Meristems. Plant and Cell Physiology. ISSN 0032-0781. 62(8), s. 1290–1301. doi: 10.1093/pcp/pcab079. Fulltekst i vitenarkiv
• Reinar, William Brynildsen; Olsson, Vilde; Reitan, Trond; Jakobsen, Kjetill Sigurd & Butenko, Melinka Alonso (2021). Length variation in short tandem repeats affects gene expression in natural populations of Arabidopsis thaliana. The Plant Cell. ISSN 1040-4651. 33(7), s. 2221–2234. doi: 10.1093/plcell/koab107. Fulltekst i vitenarkiv Vis sammendrag

  Abstract The genetic basis for the fine-tuned regulation of gene expression is complex and ultimately influences the phenotype and thus the local adaptation of natural populations. Short tandem repeats (STRs) consisting of repetitive DNA motifs have been shown to regulate gene expression. STRs are variable in length within a population and serve as a heritable, but semi-reversible, reservoir of standing genetic variation. For sessile organisms, such as plants, STRs could be of major importance in fine-tuning gene expression as a response to a shifting local environment. Here, we used a transcriptome dataset from natural accessions of Arabidopsis thaliana to investigate population-wide gene expression patterns in light of genome-wide STR variation. We empirically modeled gene expression as a response to the STR length within and around the gene and demonstrated that an association between gene expression and STR length variation is unequivocally present in the sampled population. To support our model, we explored the promoter activity in a transcriptional regulator involved in root hair formation and provided experimentally determined causality between coding sequence length variation and promoter activity. Our results support a general link between gene expression variation and STR length variation in A. thaliana.

• Olsson, Vilde; Joos, Lisa; Zhu, Shanshuo; Gevaert, Kris; Butenko, Melinka Alonso & Smet, Ive De (2019). Look closely, the beautiful may be small: Precursor-derived peptides in plants. Annual Review of Plant Biology. ISSN 1543-5008. 70, s. 153–186. doi: 10.1146/annurev-arplant-042817-040413. Fulltekst i vitenarkiv
• Shi, Chunlin; Wangenheim, Daniel von; Herrmann, Ullrich; Wildhagen, Mari; Kulik, Ivan & Kopf, Andreas [Vis alle 16 forfattere av denne artikkelen] (2018). The dynamics of root cap sloughing in Arabidopsis is regulated by peptide signalling. Nature Plants. ISSN 2055-026X. 4(8), s. 596–604. doi: 10.1038/s41477-018-0212-z. Fulltekst i vitenarkiv
• Hohmann, Ulrich; Santiago, Julia; Nicolet, Joel; Olsson, Vilde; Spiga, Fabio M & Hothorn, Ludwig A. [Vis alle 8 forfattere av denne artikkelen] (2018). Mechanistic basis for the activation of plant membrane receptor kinases by SERK-family coreceptors. Proceedings of the National Academy of Sciences of the United States of America (PNAS). ISSN 0027-8424. 115(13), s. 3488–3493. doi: 10.1073/pnas.1714972115. Fulltekst i vitenarkiv
• Mazurek, Sylwester; Garroum, Imène; Daraspe, Jean; Bellis, Damien De; Olsson, Vilde & Mucciolo, Antonio [Vis alle 9 forfattere av denne artikkelen] (2017). Connecting the molecular structure of cutin to ultrastructure and physical properties of the cuticle in petals of arabidopsis. Plant Physiology. ISSN 0032-0889. 173(2), s. 1146–1163. doi: 10.1104/pp.16.01637. Fulltekst i vitenarkiv

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• Reinar, William Brynildsen; Krabberød, Anders Kristian; Lalun, Vilde Olsson; Butenko, Melinka Alonso & Jakobsen, Kjetill Sigurd (2025). Author Correction: Short tandem repeats delineate gene bodies across eukaryotes. Nature Communications. 16(1), s. 1350–1350. doi: 10.1038/s41467-025-56684-2. Fulltekst i vitenarkiv
• Kausrud, Kyrre; Melteig, Elina; Olsson, Vilde & Galteland, Torborg (2022). Biopodden -Zoonoser. [Internet]. https://www.podbean.com/ew/pb-jddi3-11d3759. Fulltekst i vitenarkiv
• Kausrud, Kyrre; Olsson, Vilde; Melteig, Elina & Galteland, Torborg (2022). Biopodden -Skog og Klima. [Internet]. https://www.podbean.com/ew/pb-c8rck-12057a0. Fulltekst i vitenarkiv

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