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• Ekelenburg, Yuri Stephan van; Hornslien, Karina Stensland; Hautegem, Tom Van; Fendrych, Matyáš; Isterdael, Gert Van & Bjerkan, Katrine Nergård [Vis alle 9 forfattere av denne artikkelen] (2022). Spatial and temporal regulation of parent-of-origin allelic expression in the endosperm. Plant Physiology. ISSN 0032-0889. 191(2), s. 986–1001. doi: 10.1093/plphys/kiac520. Fulltekst i vitenarkiv Vis sammendrag

  Genomic imprinting promotes differential expression of parental alleles in the endosperm of flowering plants and is regulated by epigenetic modification such as DNA methylation and histone tail modifications in chromatin. After fertilization, the endosperm develops through a syncytial stage before it cellularizes and becomes a nutrient source for the growing embryo. Regional compartmentalization has been shown both in early and late endosperm development, and different transcriptional domains suggest divergent spatial and temporal regional functions. The analysis of the role of parent-of-origin allelic expression in the endosperm as a whole and the investigation of domain-specific functions have been hampered by the inaccessibility of the tissue for high-throughput transcriptome analyses and contamination from surrounding tissue. Here, we used fluorescence-activated nuclear sorting (FANS) of nuclear targeted GFP fluorescent genetic markers to capture parental-specific allelic expression from different developmental stages and specific endosperm domains. This approach allowed us to successfully identify differential genomic imprinting with temporal and spatial resolution. We used a systematic approach to report temporal regulation of imprinted genes in the endosperm, as well as region-specific imprinting in endosperm domains. Analysis of our data identified loci that are spatially differentially imprinted in one domain of the endosperm, while biparentally expressed in other domains. These findings suggest that the regulation of genomic imprinting is dynamic and challenge the canonical mechanisms for genomic imprinting.

• Bjerkan, Katrine; Hornslien, Karina Stensland; Johannessen, Ida Marie; Krabberød, Anders Kristian; Ekelenburg, Yuri Stephan van & Kalantarian, Maryam [Vis alle 11 forfattere av denne artikkelen] (2019). Genetic variation and temperature affects hybrid barriers during interspecific hybridization. The Plant Journal. ISSN 0960-7412. 101, s. 122–140. doi: 10.1111/tpj.14523. Fulltekst i vitenarkiv Vis sammendrag

  Genomic imprinting regulates parent‐specific transcript dosage during seed development and is mainly confined to the endosperm. Elucidation of the function of many imprinted genes has been hampered by the lack of corresponding mutant phenotypes, and the role of imprinting is mainly associated with genome dosage regulation or allocation of resources. Disruption of imprinted genes has also been suggested to mediate endosperm‐based post‐zygotic hybrid barriers depending on genetic variation and gene dosage. Here, we have analyzed the conservation of a clade from the MADS‐box type I class transcription factors in the closely related species Arabidopsis arenosa, A. lyrata, and A. thaliana, and show that AGL36‐like genes are imprinted and maternally expressed in seeds of Arabidopsis species and in hybrid seeds between outbreeding species. In hybridizations between outbreeding and inbreeding species the paternally silenced allele of the AGL36‐like gene is reactivated in the hybrid, demonstrating that also maternally expressed imprinted genes are perturbed during hybridization and that such effects on imprinted genes are specific to the species combination. Furthermore, we also demonstrate a quantitative effect of genetic diversity and temperature on the strength of the post‐zygotic hybridization barrier. Markedly, a small decrease in temperature during seed development increases the survival of hybrid F1 seeds, suggesting that abiotic and genetic parameters play important roles in post‐zygotic species barriers, pointing at evolutionary scenarios favoring such effects.

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• Bjerkan, Katrine; Hornslien, Karina Stensland; Johannessen, Ida Marie; Ekelenburg, Yuri Stephan van; Bramsiepe, Jonathan & Brysting, Anne Krag [Vis alle 7 forfattere av denne artikkelen] (2019). Temperature and genetic variation alter strength of the post-zygotic endosperm based barrier between A. thaliana and A.arenosa. Fulltekst i vitenarkiv
• Grini, Paul Eivind; Ekelenburg, Yuri Stephan van; Bjerkan, Katrine & Nowack, Moritz K. (2018). Analysis of Domain Specific Genomic Imprinting in the Arabidopsis Endosperm. Fulltekst i vitenarkiv
• Grini, Paul Eivind; Ekelenburg, Yuri Stephan van & Guiterrez-Marcos, Jose (2018). CAPULET2 controls endosperm development in a parent-of-origin dependent manner. Fulltekst i vitenarkiv

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