Before drugs and endogenous substances can be measured in biological samples, they have to be isolated from the original sample. In SamplePrep@UiO, we focus on development of new concepts for such isolation, based on microextraction.
Stig Pedersen-Bjergaard, Elisabeth Leere Øiestad, Astrid Gjelstad, Grete Hasvold, Magnus Saed Restan, Frederik Hansen, and Linda Vårdal Eie.
Our Mission Is to
- …develop microextraction (sample preparation) concepts for next generation analytical chemistry.
- …stimulate and train young scientist in conceptual research.
- Small, creative, effective, and with strong international recognition.
- Focused on international dissemination and collaboration.
Our research is:
- Focused on development of new analytical microextraction concepts, for extraction of drugs and endogenous substances from biological fluids (such as blood).
- Currently devoted to electromembrane extraction (EME) and liquid-phase microextraction (LPME).
- Motivated by the expectation that next generation analytical chemistry will largely rely on microextraction technologies for sample preparation.
- Conceptual, and balanced between
(a) fundamental science (theoretical understanding),
(b) applied science (technical formats, forefront pharmaceutical and biomedical applications), and
- Anchored in teaching, thereby stimulating the students in direction of pharmaceutical analytical chemistry.
- Read more about our research here.
- A day in a creative, humoristic and kind atmosphere.
- A day with new experiments.
- Our concepts are used by routine laboratories, or
- Our concepts have inspired other scientists into conceptual research, or
- Students trained in our group are successful outside the university.
Does matrix ions affect electromembrane extraction
June 23, 2020 3:56 PM
Looking through publications on electromembrane extraction (EME), it is somewhat unclear if matrix ions in the sample affect the extraction of the compounds of interest. Recently, we have looked into this very fundamental question...
New understanding how carriers work in electromembrane extraction
June 23, 2020 3:55 PM
For electromembrane extraction of polar substances, we need a carrier in the liquid membrane. Often, we use di(2-ethylhexyl) phosphate (DEHP) as carrier, but do we really know how this is working? This question we investigated recently in very fundamental work...
Eliminating changes in pH during electromembrane extraction...
June 23, 2020 3:52 PM
During electromembrane extraction (EME), electrolysis inevitably takes place in the aqueous sample and acceptor, by oxidation and reduction of water. Due to this, pH may change, and the system becomes less stable. Recently, we developed an AgCl electrode for EME to avoid pH changes. With our new electrode, electrochemistry takes place on the electrode surface rather than in water…
A new strategy...
Mar. 11, 2020 3:44 PM
In many of our research articles, the supported liquid membrane (SLM) is a key parameter when optimizing liquid-phase microextraction (LPME) or electromembrane extraction (EME) for new substances. Recently, when we optimized EME for 37 drugs of abuse in a large polarity range, we used a new and completely different strategy…
Collaboration with Chinese Scientists from Wuhan…
Mar. 11, 2020 2:40 PM
Wuhan has been in focus the last couple of months due to virus, but Wuhan is also a very active spot in development of electromembrane extraction (EME). Collaboration with this Chinese EME hub is extremely important, and one example was published recently in Journal of Membrane Science…
Moving analytical chemistry out of the laboratory…
Mar. 11, 2020 2:40 PM
Smartphones will be very important for chemical measurements in the future. For patients, smartphones may be used to check that they are properly medicated, based on measurement from a single blood droplet obtained by a finger prick. Concentration measurement of drugs, they can do themselves in the future, provided that small devices are developed, where the drug substances are separated from all the other compounds present in blood, before measurement. In our newest research, we took a small step in this direction…
A lot of reviews...
Mar. 11, 2020 2:40 PM
The last year has been very intensive in terms of writing reviews. Together with international collaborators, we have reviewed “emerging extraction strategies” for Analytical Chemistry, “microextraction in bioanalysis” for Journal of Chromatography A, “hollow-fibre liquid-phase microextraction” for Journal of Pharmaceutical Analysis, and finally “mass spectrometric measurements from organ-on-chip systems” for Electrophoresis. For us it has been a great opportunity to get fully updated in areas where we are doing experimental work… Hopefully, many scientists will find valuable information and inspiration in the reviews...
Is this the final jump of LPME into routine?
Mar. 11, 2020 2:38 PM
We have been working with liquid-phase microextraction (LPME) for a long time, and LPME can now be done with commercial equipment in 96-well format, and it can be automated. Getting LPME into routine laboratories is a final goal for us, and in this direction we recently tested and documented the reliability of LPME combined with LC-MS for measurement of antidepressants in human blood. Testing was done according to industrial guidelines, and involved blood samples from different patients, and data were in compliance with routine laboratory requirements…
Moving EME into protein analysis…
Mar. 11, 2020 2:38 PM
In biochemical research, proteins are often labelled with a fluorescent compound for visualisation. However, prior to visualisation, excess of label has to be removed to reduce the background fluorescence. In a recent work published in Analytical Chemistry, we tested the idea of using electromembrane extraction (EME) for such purposes. The philosophy behind this work is to push EME in new directions, and to inspire other scientists to explore and develop new applications of EME. Some of these future applications will hopefully be “killer applications”, and EME will be an established technique in the analytical toolbox…
Analytical chemistry with smartphones…
Nov. 26, 2019 9:20 PM
With our smartphone, we can send text messages, google, and talk, and do many other things…but in some years we can even use the smartphone for measurement of chemicals and pharmaceuticals. Thus, non-trained persons may in the future conduct analytical measurements currently done by trained scientists in specialized laboratories, on-site. This may open new doors, but there are many challenges…