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Reubsaet, Leon
(2024).
Smart Sampling: next generation DBS samplers for LC-MS based protein analysis.
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Halvorsen, Trine Grønhaug & Reubsaet, Leon
(2023).
Next generation dried blood spot samplers for simplified LC-MS/MS analysis of proteins.
-
Halvorsen, Trine Grønhaug & Reubsaet, Leon
(2023).
Smart samplers for simplified LC-MS/MS analysis of proteins.
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Reubsaet, Leon & Halvorsen, Trine Grønhaug
(2023).
Smart sampling: next-generation DBS samplers for LC-MS based protein analysis .
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Halvorsen, Trine Grønhaug & Reubsaet, Leon
(2023).
Smart samplers for mass spectrometric bioanalysis of proteins.
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Halvorsen, Trine Grønhaug & Reubsaet, Leon
(2022).
Syntetisk antistoff forenklar analyse av blodprøve.
Forskning.no.
ISSN 1891-635X.
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Reubsaet, Leon & Halvorsen, Trine Grønhaug
(2022).
Smart samplers for LC-MS based protein analysis.
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Halvorsen, Trine Grønhaug & Reubsaet, Leon
(2022).
Smart samplers in mass spectrometric bioanalysis of proteins.
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Reubsaet, Leon & Halvorsen, Trine Grønhaug
(2022).
Smart Samplers for LC-MS based protein analysis. .
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Reubsaet, Leon
(2022).
Hvordan vedlikeholder jeg LC-MS instrumentet.
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Reubsaet, Leon & Halvorsen, Trine Grønhaug
(2022).
Smart Samplers for LC-MS based protein analysis.
-
Reubsaet, Leon & Halvorsen, Trine Grønhaug
(2022).
Smart Sampler for LC-MS based protein analysis.
-
Reubsaet, Leon & Halvorsen, Trine Grønhaug
(2022).
Smart samplers for LC-MS based protein analysis.
-
Reubsaet, Leon
(2022).
Smart Sampler for LC-MS based protein analysis.
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Reubsaet, Leon
(2022).
Smart Sampler for LC-MS based protein analysis.
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Vik, Anders; Halvorsen, Trine Grønhaug; Primdahl, Karoline Gangestad; Hansen, Trond Vidar; Wangensteen, Helle & Reubsaet, Leon
[Vis alle 7 forfattere av denne artikkelen]
(2022).
Grunnkurs i farmasøytisk kjemi på UiO moderniseres kontinuerlig.
Norsk Farmaceutisk Tidsskrift.
ISSN 0029-1935.
s. 33–34.
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Halvorsen, Trine Grønhaug & Reubsaet, Leon
(2021).
Advances in sampling and sample preparation for mass spectrometric determination of protein biomarkers.
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Bakketeig, Eris Aas; Olsen, Christine; Berg, Henriette Engen; Nyman, Tuula Anneli; Thiede, Bernd & Lundanes, Elsa
[Vis alle 8 forfattere av denne artikkelen]
(2021).
Optimization of concatenation of fractions in
comprehensive two dimensional liquid chromatography
bottom-up proteomics.
Vis sammendrag
Two dimensional liquid chromatography (2D-LC) has long been a commonly used technique
in bottom-up proteomics, and is as relevant today as ever [1, 2]. To maximize the number of
protein identifications, analytical chemists have strived to obtain 2D-LC systems with a high
degree of orthogonality, which is needed to reach the required high peak capacities. A system
utilizing reversed-phase chromatography (RP) in both column dimensions with a large
difference in pH has proven to yield a higher peak capacity for peptides, even with a lower
degree of orthogonality compared to other combinations [3].
The present study is based on Reubsaet, et al. [4]. The study is a comprehensive offline RPRP 2D-LC system with high pH (>9) in the first dimension and low pH (3) in the second
dimension. The method’s purpose is to maximise protein identifications from one trypsinated
protein sample. To achieve maximum identifications, Reubsaet incorporated the concept of
concatenation of fractions. In practice, concatenation is applied through collecting the column
output from the first dimension in eight numbered vials, where the 1st, 9th, 17th, … fractions
are collected in the first vial, the 2nd, 10th, 18th, … are collected in the second, etc. [4].
In the present study, the fractionation scheme is examined by changing the total number of
vials in which samples are concatenated, the number of fractions in each vial, and the
collection time for each fraction. As the method demands high precision and is very timeconsuming, automatization is practically essential. Automatization is achieved with the spider
fractionation scheme, proposed and shown by Kulak, et al.[5]. Here, a 10-port injector
connects the first dimension column to each sample vial, and switches between them
automatically, reducing the strain on the operator drastically, while removing human errors.
The optimization of the concatenation will lead to higher numbers of identified proteins in
proteomics.
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Reubsaet, Leon
(2020).
Ekstremt små blodprøvar kan analyserast utan tap av informasjon.
[Internett].
Titan.uio.no.
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Reubsaet, Leon & Halvorsen, Trine Grønhaug
(2019).
Affinity based sample clean-up prior to LC-MS/MS for determination of low abundance proteins.
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Reubsaet, Leon
(2019).
Forsker på ny teknologi for diagnostikk i Los Angeles.
[Avis].
Dagens Medisin.
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Skjærvø, Øystein; Halvorsen, Trine Grønhaug & Reubsaet, Leon
(2019).
Smart blood spot sampling for MS tased bottom-up protein analysis.
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Skjærvø, Øystein; Halvorsen, Trine Grønhaug & Reubsaet, Leon
(2019).
Smart blood-spot sampling for MS based bottom-up protein analysis.
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Skjærvø, Øystein; Pilarova, Veronika; Khalikova, Maria; Reubsaet, Leon; Halvorsen, Trine Grønhaug & Gjelstad, Astrid
[Vis alle 10 forfattere av denne artikkelen]
(2018).
Those Who Can, Teach: Pharma Stars.
The Analytical Scientist.
ISSN 2051-4077.
1118,
s. 40–45.
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Skjærvø, Øystein; Pilarova, Veronika; Khalikova, Maria; Reubsaet, Leon; Halvorsen, Trine Grønhaug & Gjelstad, Astrid
[Vis alle 10 forfattere av denne artikkelen]
(2018).
Those Who Can, Teach: Pharma Stars.
The Analytical Scientist.
ISSN 2051-4077.
1118,
s. 40–45.
-
Brandtzaeg, Ole Kristian; Levernæs, Maren Christin Stillesby; Halvorsen, Trine Grønhaug; Reubsaet, Leon; Røen, Bent Tore & Vehus, Tore
[Vis alle 9 forfattere av denne artikkelen]
(2018).
Online sample processing systems for targeted nano liquid chromatography mass spectrometry bioanalysis.
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Rossetti, Cecilia; Ore, Odd Gøran; Sellergren, Börje; Halvorsen, Trine Grønhaug & Reubsaet, Leon
(2017).
Correction to: Exploring the peptide retention mechanism in molecularly imprinted polymers (Analytical and Bioanalytical Chemistry, (2017), 409, 24, (5631-5643), 10.1007/s00216-017-0520-6).
Analytical and Bioanalytical Chemistry.
ISSN 1618-2642.
410(5),
s. 1607–1608.
doi:
10.1007/s00216-017-0797-5.
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Halvorsen, Trine Grønhaug; Rossetti, Cecilia; Levernæs, Maren Christin Stillesby & Reubsaet, Leon
(2016).
The Impact of Sample Clean-up Technique in Bottom-up based LC-MS Analysis of Low Abundance Serum Biomarkers
.
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Myhrer, Marthe; Krogstad, Veronica; Christensen, Hege Staaland; Reubsaet, Leon & Hermann, Monica
(2015).
Kvantifisering av P-glykoprotein i lymfocytter ved bruk av LC-MS/MS.
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Reubsaet, Leon & Halvorsen, Trine Grønhaug
(2015).
Analysis of Peptide and Protein Drugs in Biological Fluids.
I Hansen, Steen Honoré & Pedersen-Bjergaard, Stig (Red.),
Bioanalysis of pharmaceuticals : sample preparation, separation techniques and mass spectrometry.
Wiley-Blackwell.
ISSN 978-111871682-3.
s. 261–282.
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Reubsaet, Leon
(2015).
Targeted Proteomics - Immunoaffinity sample preparation and isoform differentiation.
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Halvorsen, Trine Grønhaug; Egeland, Siri Valen & Reubsaet, Leon
(2015).
Dual immunocapture combined with targeted LC-MS/MS for increased differentiation power in protein biomarker determination.
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Brandtzæg, Ole Kristian Merkesvik; Hustoft, Hanne Kolsrud; Vehus, Tore; Greibrokk, Tyge; Krauss, Stefan & Reubsaet, Leon
[Vis alle 8 forfattere av denne artikkelen]
(2015).
On-line protein digestion and peptide separation using “ultra-nano” LC-MS
.
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Halvorsen, Trine Grønhaug; Lund, Hanne & Reubsaet, Leon
(2014).
Immunoaffinity + LC-MS/MS: An alternative approach for antibody selectivity evaluation?
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Hustoft, Hanne Kolsrud; Brandtzæg, Ole Kristian Merkesvik; Torsetnes, Silje Bøen; Greibrokk, Tyge; Reubsaet, Leon & Wilson, Steven Ray Haakon
[Vis alle 7 forfattere av denne artikkelen]
(2014).
Development of a nanoproteomic platform with integrated immunocapture and enzymatic digestion for on-line detection of the low abundant cancer biomarker PROGRP.
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Reubsaet, Leon
(2014).
Bestemmelse av proteiner ved hjelp av LC-MS.
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Jensen, Ben Andre; Reubsaet, Leon; Christensen, Hege Staaland; Åsberg, Anders & Hermann, Monica
(2013).
Utvikling av kvantitativ proteomikkmetode for måling av P-glykoprotein i lymfocytter isolert fra pasienter.
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Halvorsen, Trine Grønhaug; Lund, Hanne & Reubsaet, Leon
(2013).
Targeted biomarker analysis by combined immunoaffinity and LC-MS/MS.Potential and possibilities.
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Torsetnes, Silje Bøen; Halvorsen, Trine Grønhaug; Reubsaet, Leon; Paus, Elisabeth; Nordlund, Marianne Sparby & Levernæs, Maren C. S.
(2013).
”Breaking barriers in cancer marker determination, Simultaneous differentiation of ProGRP isoforms and NSE isoenzymes by bottom-up immuno-LC-MS/MS”, M. C. S. Levernæs, S. B. Torsetnes, M. S. Nordlund, T. G. Halvorsen, E. Paus, L. Reubsaet. NCMS (Nordic NSMS), Hafjell, 2013.
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Seip, Knut Fredrik; Bjerknes, Kari Christiane; Johansen, Harald Thidemann; Landrø, Linn Aina Ysland; Helsing, Per & Reubsaet, Leon
[Vis alle 7 forfattere av denne artikkelen]
(2013).
Investigating bradykinin exposure in hereditary angioedema through the metabolite BK1-5.
-
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Jensen, Ben Andre; Hermann, Monica; Reubsaet, Leon & Christensen, Hege Staaland
(2013).
Utvikling av kvantitativ proteomikkmetodefor P-glykoprotein i mononukleære celler fra perifert blod.
Universitetet i Oslo.