Ayushi Sowmya Ramachandran

• Ramachandran, Sowmya; Krogh, Nicolai; Jørgensen, Tor Erik; Johansen, Steinar Daae; Nielsen, Henrik & Babiak, Igor (2020). The shift from early to late types of ribosomes in zebrafish development involves changes at a subset of rRNA 2′-O-Me sites. RNA: A publication of the RNA Society. ISSN 1355-8382. 26(12), s. 1919–1934. doi: 10.1261/rna.076760.120. Fulltekst i vitenarkiv Vis sammendrag

  During zebrafish development, an early type of rRNA is gradually replaced by a late type that is substantially different in sequence. We applied RiboMeth-seq to rRNA from developmental stages for profiling of 2′-O-Me, to learn if changes in methylation pattern were a component of the shift. We compiled a catalog of 2′-O-Me sites and cognate box C/D guide RNAs comprising 98 high-confidence sites, including 10 sites that were not known from other vertebrates, one of which was specific to late-type rRNA. We identified a subset of sites that changed in methylation status during development and found that some of these could be explained by availability of their cognate SNORDs. Sites that changed during development were enriched in the novel sites revealed in zebrafish. We propose that the early type of rRNA is a specialized form and that its structure and ribose methylation pattern may be an adaptation to features of development, including translation of specific maternal mRNAs.

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• Ramachandran, Sowmya; Babiak, Igor; Johansen, Steinar Daae & Nielsen, Henrik (2022). Ribosomal RNA, ribose methylation, and box C/D snoRNAs during embryonic development of teleosts zebrafish (Danio rerio) and medaka (Oryzias latipes). Nord universitet. ISSN 9788293165453. Fulltekst i vitenarkiv

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