Pierre Chymkowitch

• Nguéa P, Aurélie; Robertson, Joseph; Herrera, Maria Carmen; Chymkowitch, Pierre & Enserink, Jorrit (2019). Desumoylation of RNA polymerase III lies at the core of the Sumo stress response in yeast. Journal of Biological Chemistry.  ISSN 0021-9258.  294(49), s 18784- 18795 . doi: 10.1074/jbc.RA119.009721 Full text in Research Archive.
• Chymkowitch, Pierre & Enserink, Jorrit (2018). Regulation of tRNA synthesis by posttranslational modifications of RNA polymerase III subunits.. Biochimica et Biophysica Acta. Gene Regulatory Mechanisms.  ISSN 1874-9399.  1861(4), s 310- 319 . doi: 10.1016/j.bbagrm.2017.11.001 Show summary

  RNA polymerase III (RNAPIII) transcribes tRNA genes, 5S RNA as well as a number of other non-coding RNAs. Because transcription by RNAPIII is an energy-demanding process, its activity is tightly linked to the stress levels and nutrient status of the cell. Multiple signaling pathways control RNAPIII activity in response to environmental cues, but exactly how these pathways regulate RNAPIII is still poorly understood. One major target of these pathways is the transcriptional repressor Maf1, which inhibits RNAPIII activity under conditions that are detrimental to cell growth. However, recent studies have found that the cell can also directly regulate the RNAPIII machinery through phosphorylation and sumoylation of RNAPIII subunits. In this review we summarize post-translational modifications of RNAPIII subunits that mainly have been identified in large-scale proteomics studies, and we highlight several examples to discuss their relevance for regulation of RNAPIII.

• Herrera, Maria Carmen; Chymkowitch, Pierre; Robertson, Joseph; Eriksson, Jens; Bøe, Stig Ove; Alseth, Ingrun & Enserink, Jorrit (2018). Cdk1 gates cell cycle-dependent tRNA synthesis by regulating RNA polymerase III activity. Nucleic Acids Research (NAR).  ISSN 0305-1048.  46(22), s 11698- 11711 . doi: 10.1093/nar/gky846 Full text in Research Archive.
• Kruitwagen, Tom; Chymkowitch, Pierre; Denoth-Lippuner, Annina; Enserink, Jorrit & Barral, Yves (2018). Centromeres License the Mitotic Condensation of Yeast Chromosome Arms. Cell.  ISSN 0092-8674.  175(3), s 780- 795.e15 . doi: 10.1016/j.cell.2018.09.012 Full text in Research Archive.
• Chymkowitch, Pierre; Nguéa P, Aurélie; Aanes, Håvard; Robertson, Joseph; Klungland, Arne & Enserink, Jorrit (2017). TORC1-dependent sumoylation of Rpc82 promotes RNA polymerase III assembly and activity. Proceedings of the National Academy of Sciences of the United States of America.  ISSN 0027-8424.  114(5), s 1039- 1044 . doi: 10.1073/pnas.1615093114
• Zimmermann, Christine; Garcia Llorente, Ignacio; Omerzu, Manja; Chymkowitch, Pierre; Zhang, Beibei & Enserink, Jorrit (2017). Mapping the synthetic dosage lethality network of CDK1/CDC28. G3: Genes, Genomes, Genetics.  ISSN 2160-1836.  7(6), s 1753- 1766 . doi: 10.1534/g3.117.042317 Full text in Research Archive.
• Chymkowitch, Pierre; Nguéa P, Aurélie; Aanes, Håvard; Koehler, Christian; Thiede, Bernd; Lorenz, Susanne; Meza, Leonardo Zepeda; Klungland, Arne & Enserink, Jorrit (2015). Sumoylation of Rap1 mediates the recruitment of TFIID to promote transcription of ribosomal protein genes. Genome Research.  ISSN 1088-9051.  25(6), s 897- 906 . doi: 10.1101/gr.185793.114
• Chymkowitch, Pierre; Nguéa P, Aurélie & Enserink, Jorrit (2015). SUMO-regulated transcription: Challenging the dogma. Bioessays.  ISSN 0265-9247.  37(10), s 1095- 1105 . doi: 10.1002/bies.201500065
• Chymkowitch, Pierre & Enserink, Jorrit (2013). The cell cycle rallies the transcription cycle: Cdc28/Cdk1 is a cell cycle-regulated transcriptional CDK. Transcription.  ISSN 2154-1264.  4(1), s 3- 6 . doi: 10.4161/trns.22456
• Zimmermann, Christine; Santos, Aline; Gable, Kenneth; Epstein, Sharon; Gururaj, Charulatha; Chymkowitch, Pierre; Pultz, Dennis; Rødkær, Steven V.; Clay, Lorena; Bjørås, Magnar; Barral, Yves; Chang, Amy; Færgeman, Nils J.; Dunn, Teresa M.; Riezman, Howard & Enserink, Jorrit (2013). TORC1 Inhibits GSK3-Mediated Elo2 Phosphorylation to Regulate Very Long Chain Fatty Acid Synthesis and Autophagy. Cell reports.  ISSN 2211-1247.  5(4), s 1036- 1046 . doi: 10.1016/j.celrep.2013.10.024
• Chymkowitch, Pierre; Eldholm, Vegard; Lorenz, Susanne; Zimmermann, Christine; Lindvall, Jessica M.; Bjørås, Magnar; Meza-Zepeda, Leonardo Andrés & Enserink, Jorrit (2012). Cdc28 kinase activity regulates the basal transcription machinery at a subset of genes. Proceedings of the National Academy of Sciences of the United States of America.  ISSN 0027-8424.  109(26), s 10450- 10455 . doi: 10.1073/pnas.1200067109
• Zimmermann, Christine; Chymkowitch, Pierre; Eldholm, Vegard; Putnam, Christopher D.; Lindvall, Jessica Margareta; Omerzu, Manja; Bjørås, Magnar; Kolodner, Richard D. & Enserink, Jorrit (2011). A chemical-genetic screen to unravel the genetic network of CDC28/CDK1 links ubiquitin and Rad6-Bre1 to cell cycle progression. Proceedings of the National Academy of Sciences of the United States of America.  ISSN 0027-8424.  108(46), s 18748- 18753 . doi: 10.1073/pnas.1115885108

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• Zimmermann, Christine; Chymkowitch, Pierre; Eldholm, Vegard; Putnam, Christopher D.; Lindvall, Jessica M.; Omerzu, Manja; Bjørås, Magnar; Kolodner, Richard D. & Enserink, Jorrit (2012). A chemical-genetic screen to unravel the genetic network of CDC28/CDK1 links ubiquitin and Rad6-Bre1 to cell cycle progression. The FASEB Journal.  ISSN 0892-6638.  26

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