Erik Boye

• Nissen-Meyer, Jon; Skotland, Tore & Boye, Erik (2022). Are doping tests in sports trustworthy?: Athletes suffer from insufficiently defined criteria for doping tests: Athletes suffer from insufficiently defined criteria for doping tests. EMBO Reports. ISSN 1469-221X. 23. doi: 10.15252/embr.202154431. Fulltekst i vitenarkiv
• Anda, Silje; Boye, Erik; Schink, Kay Oliver & Grallert, Beata (2021). Cosegregation of asymmetric features during cell division. Open Biology. 11(8), s. 1–11. doi: 10.1098/rsob.210116. Fulltekst i vitenarkiv Vis sammendrag

  Cellular asymmetry plays a major role in the ageing and evolution of multicellular organisms. However, it remains unknown how the cell distinguishes ‘old’ from ‘new’ and whether asymmetry is an attribute of highly specialized cells or a feature inherent in all cells. Here, we investigate the segregation of three asymmetric features: old and new DNA, the spindle pole body (SPB, the centrosome analogue) and the old and new cell ends, using a simple unicellular eukaryote, Schizosaccharomyces pombe. To our knowledge, this is the first study exploring three asymmetric features in the same cells. We show that of the three chromosomes of S. pombe, chromosome I containing the new parental strand, preferentially segregated to the cells inheriting the old cell end. Furthermore, the new SPB also preferentially segregated to the cells inheriting the old end. Our results suggest that the ability to distinguish ‘old’ from ‘new’ and to segregate DNA asymmetrically are inherent features even in simple unicellular eukaryotes.

• Boye, Erik & Grallert, Beata (2019). eIF2α phosphorylation and the regulation of translation. Current Genetics. ISSN 0172-8083. s. 1–5. doi: 10.1007/s00294-019-01026-1. Fulltekst i vitenarkiv
• Pielke, Roger; Tucker, Ross & Boye, Erik (2019). Scientific integrity and the IAAF testosterone regulations. The International Sports Law Journal. ISSN 1567-7559. doi: 10.1007/s40318-019-00143-w. Fulltekst i vitenarkiv
• Arnason_Bøe, Cathrine; Håland, Tine Weise; Boye, Erik; Syljuåsen, Randi G. & Grallert, Beata (2018). A novel role for ATR/Rad3 in G1 phase. Scientific Reports. 8(1). doi: 10.1038/s41598-018-25238-6. Fulltekst i vitenarkiv
• Stonyte, Vilte; Boye, Erik & Grallert, Beata (2018). Regulation of global translation during the cell cycle. Journal of Cell Science. ISSN 0021-9533. 131(17), s. 1–9. doi: 10.1242/jcs.220327. Fulltekst i vitenarkiv
• Boye, Erik; Skotland, Tore; Østerud, Bjarne & Nissen-Meyer, Jon (2017). Doping and drug testing. EMBO Reports. ISSN 1469-221X. 18(3), s. 351–354. doi: 10.15252/embr.201643540. Fulltekst i vitenarkiv
• Rothe, Christiane; Rødland, Gro Elise; Anda, Silje; Stonyte, Vilte; Boye, Erik & Lopez-Aviles, Sandra [Vis alle 7 forfattere av denne artikkelen] (2017). A checkpoint-independent mechanism delays entry into mitosis after UV irradiation. Journal of Cell Science. ISSN 0021-9533. 130(23), s. 4028–4037. doi: 10.1242/jcs.204693. Fulltekst i vitenarkiv
• Anda, Silje; Rothe, Christiane; Boye, Erik & Grallert, Beata (2016). Consequences of abnormal CDK activity in S phase. Cell Cycle. ISSN 1538-4101. 15(7), s. 963–973. doi: 10.1080/15384101.2016.1152423. Fulltekst i vitenarkiv
• Boye, Erik; Anda, Silje; Rothe, Christiane; Stokke, Trond & Grallert, Beata (2016). Analyzing Schizosaccharomyces pombe DNA content by flow cytometry. Cold Spring Harbor Protocols. ISSN 1940-3402. 2016(6), s. 520–524. doi: 10.1101/pdb.prot091280. Fulltekst i vitenarkiv
• Nissen-Meyer, Jon; Boye, Erik; Østerud, Bjarne & Skotland, Tore (2016). EPO Testing in Doping Control Laboratories is Absolutely No Joke. Lab Times. ISSN 1864-2381. 2016-6, s. 28–29. Fulltekst i vitenarkiv
• Nissen-Meyer, Jon; Boye, Erik; Østerud, Bjarne & Skotland, Tore (2016). Another troubling doping case is questioning WADA’s credibility again: Borderline Analysis. Lab Times. ISSN 1864-2381. 2016-5, s. 16–19. Fulltekst i vitenarkiv
• Knutsen, Jon Halvor Jonsrud; Rødland, Gro Elise; Bøe, Cathrine Arnason; Håland, Tine Weise; Sunnerhagen, Per & Grallert, Beata [Vis alle 7 forfattere av denne artikkelen] (2015). Stress-induced inhibition of translation independently of eIF2α phosphorylation. Journal of Cell Science. ISSN 0021-9533. 128(23), s. 4420–4427. doi: 10.1242/jcs.176545. Fulltekst i vitenarkiv
• Nissen-Meyer, Jon; Boye, Erik; Østerud, Bjarne & Skotland, Tore (2015). Problems at a WADA-accredited anti-doping lab: Puzzling Discrepancy. Lab Times. ISSN 1864-2381. 2015-5, s. 18–23. Fulltekst i vitenarkiv
• Håland, Tine Weise; Boye, Erik; Stokke, Trond; Grallert, Beata & Syljuåsen, Randi G. (2015). Simultaneous measurement of passage through the restriction point and MCM loading in single cells. Nucleic Acids Research (NAR). ISSN 0305-1048. 43:e150(22), s. 1–11. doi: 10.1093/nar/gkv744. Fulltekst i vitenarkiv Vis sammendrag

  Passage through the Retinoblastoma protein (RB1)-dependent restriction point and the loading of minichromosome maintenance proteins (MCMs) are two crucial events in G1-phase that help maintain genome integrity. Deregulation of these processes can cause uncontrolled proliferation and cancer development. Both events have been extensively characterized individually, but their relative timing and inter-dependence remain less clear. Here, we describe a novel method to simultaneously measure MCM loading and passage through the restriction point. We exploit that the RB1 protein is anchored in G1-phase but is released when hyper-phosphorylated at the restriction point. After extracting cells with salt and detergent before fixation we can simultaneously measure, by flow cytometry, the loading of MCMs onto chromatin and RB1 binding to determine the order of the two events in individual cells. We have used this method to examine the relative timing of the two events in human cells. Whereas in BJ fibroblasts released from G0-phase MCM loading started mainly after the restriction point, in a significant fraction of exponentially growing BJ and U2OS osteosarcoma cells MCMs were loaded in G1-phase with RB1 anchored, demonstrating that MCM loading can also start before the restriction point. These results were supported by measurements in synchronized U2OS cells.

• Anda, Silje; Boye, Erik & Grallert, Beata (2014). Cell-cycle analyses using thymidine analogues in fission yeast. PLOS ONE. 9(2). doi: 10.1371/journal.pone.0088629. Fulltekst i vitenarkiv
• Rødland, Gro Elise; Tvegård, Tonje; Boye, Erik & Grallert, Beata (2014). Crosstalk between the Tor and Gcn2 pathways in response to different stresses. Cell Cycle. ISSN 1538-4101. 13(3), s. 453–461. doi: 10.4161/cc.27270. Fulltekst i vitenarkiv
• Nissen-Meyer, Jon; Boye, Erik; Østerud, Bjarne & Skotland, Tore (2013). WADA-accredited doping analyses cannot always be trusted. Lab Times. ISSN 1864-2381. 2013-1, s. 18–23. Fulltekst i vitenarkiv
• Grallert, Beata & Boye, Erik (2013). GCN2, an old dog with new tricks. Biochemical Society Transactions. ISSN 0300-5127. 41, s. 1687–1691. doi: 10.1042/BST20130210. Fulltekst i vitenarkiv
• Bøe, Cathrine Arnason; Knutsen, Jon Halvor Jonsrud; Boye, Erik & Grallert, Beata (2012). Hpz1 Modulates the G1-S Transition in Fission Yeast. PLOS ONE. 7(9:e44539). doi: 10.1371/journal.pone.0044539. Fulltekst i vitenarkiv
• Bøe, Cathrine Arnason; Krohn, Marit; Rødland, Gro Elise; Capiaghi, Cristoph; Maillard, Olivier & Thoma, Fritz [Vis alle 8 forfattere av denne artikkelen] (2012). Induction of a G(1)-S checkpoint in fission yeast. Proceedings of the National Academy of Sciences of the United States of America (PNAS). ISSN 0027-8424. 109(25), s. 9911–9916. doi: 10.1073/pnas.1204901109. Fulltekst i vitenarkiv
• Knutsen, Jon Halvor Jonsrud; Rein, Idun Dale; Rothe, Christiane; Stokke, Trond; Grallert, Beata & Boye, Erik (2011). Cell-Cycle Analysis of Fission Yeast Cells by Flow Cytometry. PLOS ONE. 6(2). doi: 10.1371/journal.pone.0017175. Fulltekst i vitenarkiv Vis sammendrag

  The cell cycle of the fission yeast, Schizosaccharomyces pombe, does not easily lend itself to analysis by flow cytometry, mainly because cells in G1 and G2 phase contain the same amount of DNA. This occurs because fission yeast cells under standard growth conditions do not complete cytokinesis until after G1 phase. We have devised a flow cytometric method exploiting the fact that cells in G1 phase contain two nuclei, whereas cells in G2 are mononuclear. Measurements of the width as well as the total area of the DNA-associated fluorescence signal allows the discrimination between cells in G1 and in G2 phase and the cell-cycle progression of fission yeast can be followed in detail by flow cytometry. Furthermore, we show how this method can be used to monitor the timing of cell entry into anaphase. Fission yeast cells tend to form multimers, which represents another problem of flow cytometry-based cell-cycle analysis. Here we present a method employing light-scatter measurements to enable the exclusion of cell doublets, thereby further improving the analysis of fission yeast cells by flow cytometry. © 2011 Knutsen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

• Boye, Erik & Grallert, Beata (2009). In DNA replication, the early bird catches the worm. Cell. ISSN 0092-8674. 136, s. 812–814. Fulltekst i vitenarkiv
• Skjølberg, Henriette C.; Fensgård, Øyvind; Nilsen, Hilde; Grailert, Beata & Boye, Erik (2009). Global transcriptional response after exposure of fission yeast cells to ultraviolet light. BMC Cell Biology. 10, s. 87–87. doi: 10.1186/1471-2121-10-87. Fulltekst i vitenarkiv Vis sammendrag

  Background In many cell types, including the fission yeast Schizosaccharomyces pombe, a set of checkpoints are induced by perturbations of the cell cycle or by DNA damage. Many of the checkpoint responses include a substantial change of the transcriptional pattern. As part of characterising a novel G1/S checkpoint in fission yeast we have investigated whether a transcriptional response is induced after irradiation with ultraviolet light. Results Microarray analyses were used to measure the global transcription levels of all open reading frames of fission yeast after 254 nm ultraviolet irradiation, which is known to induce a G1/S checkpoint. We discovered a surprisingly weak transcriptional response, which is quite unlike the marked changes detected after some other types of treatment and in several other checkpoints. Interestingly, the alterations in gene expression after ultraviolet irradiation were not similar to those observed after ionising radiation or oxidative stress. Pathway analysis suggests that there is little systematic transcriptional response to the irradiation by ultraviolet light, but a marked, coordinated transcriptional response was noted on progression of the cells from G1 to S phase. Conclusion There is little response in fission yeast to ultraviolet light at the transcriptional level. Amongst the genes induced or repressed after ultraviolet irradiation we found none that are likely to be involved in the G1/S checkpoint mechanism, suggesting that the checkpoint is not dependent upon transcriptional regulation.

• Boye, Erik; Corneliussen, Henriette & Grallert, Beata (2009). Checkpoint regulation of DNA replication. Methods in molecular biology. ISSN 1064-3745. 521, s. 55–70. Fulltekst i vitenarkiv
• Stepankiw, N; Kaidow, A; Cilkeman, J.A.; Boye, Erik & Bates, David (2009). The right half of the Escherichia coli replication origin is not essential for viability, but facilitates multi-forked replication. Molecular Microbiology. ISSN 0950-382X. 74, s. 467–479. Fulltekst i vitenarkiv
• Tvegård, Tonje; Soltani, H; Corneliussen, Henriette; Krohn, Marit; Nilssen, EA & Kearsey, SE [Vis alle 8 forfattere av denne artikkelen] (2007). A novel checkpoint mechanism regulating the G1/S transition. Genes & Development. ISSN 0890-9369. 21. doi: 10.1101/gad.421807. Fulltekst i vitenarkiv
• Nilssen, Esben A.; Emblemsvåg, Marianne Synnes; Tvegård, Tonje; Vebø, Heidi; Boye, Erik & Grallert, Beata (2004). Germinating fission yeast spores delay in G1 in responsesto UV irradiation. BMC Cell Biology. 5(40), s. 1471–2121. Fulltekst i vitenarkiv
• Emblemsvåg, Marianne Synnes; Nilssen, Esben A.; Boye, Erik & Grallert, Beata (2002). A novel chk1-dependent G1/M checkpoint in fission yeast. Journal of Cell Science. ISSN 0021-9533. 15(115), s. 3609–3618. Fulltekst i vitenarkiv

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• Bustad, Helene J.; Flydal, Marte Innselset; Knævelsrud, Helene; Langaker, Ann Helen; Boye, Erik & Kristensen, Tom (2022). Slik utvikler vi norske fagtermer i celle-og molekylærbiologi. Tidsskrift for Den norske legeforening (Tidsskriftet). ISSN 0029-2001. 142(2). doi: 10.4045/tidsskr.21.0465. Fulltekst i vitenarkiv
• Bustad, Helene J.; Flydal, Marte Innselset; Knævelsrud, Helene; Boye, Erik & Kristensen, Tom Arne (2021). Et kappløp for norsk fagspråk. Michael. ISSN 1893-9651. 18(26), s. 81–91. Fulltekst i vitenarkiv
• Pielke, Roger; Tucker, Ross & Boye, Erik (2019). Correction to: Scientific integrity and the IAAF testosterone regulations (The International Sports Law Journal, (2019), 19, 1-2, (18-26), 10.1007/s40318-019-00143-w). The International Sports Law Journal. ISSN 1567-7559. 19(1-2), s. 27–28. doi: 10.1007/s40318-019-00149-4. Fulltekst i vitenarkiv
• Nissen-Meyer, Jon; Skotland, Tore; Østerud, Bjarne & Boye, Erik (2019). Improving scientific practice in sports-associated drug testing. The FEBS Journal. ISSN 1742-464X. s. 1–6. doi: 10.1111/febs.14920. Fulltekst i vitenarkiv
• Tilset, Mats; Boye, Erik & Spurkland, Anne (2013). Glemte sykdommer rammer 1 milliard. Fulltekst i vitenarkiv
• Føllesdal, Andreas & Boye, Erik (2011). Forskerråd til forskningsrådet. [Journal]. Morgenbladet. Fulltekst i vitenarkiv Vis sammendrag

  For at det norske demokratiske velferdssamfunnet skal være bærekraftig, må vi holde oss med fremragende forskning og forskningsbasert undervisning. Hvordan bør vi styre forskningen i Norge? For å takle fremtidens utfordringer trenger vi kloke forskningspolitiske rådgivere og dyktige forvaltere av de offentlige forskningsmidlene. I Norge kanaliseres det aller meste av slike midler gjennom Norges forskningsråd (NFR). Nå skal NFR evalueres. Det gir anledning til å gjøre opp status og vurdere veivalg videre.

• Abrahamsen, Torveig Weum; Tvegård, Tonje; Syljuåsen, Randi G.; Grallert, Beata & Boye, Erik (2010). The role of GCN2 in cell-cycle regulation in mammalian cells. The FEBS Journal. ISSN 1742-464X. 277, s. 133–133. Fulltekst i vitenarkiv
• Boye, Erik (2004). Hvilke forskningsresultater kan vi stole på? Fulltekst i vitenarkiv

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