elief

• Cortes, S.; Farhat, Elie; Talarico, G.G.M. & Mennigen, J.A. (2024). The dynamic transcriptomic response of the goldfish brain under chronic hypoxia. Comparative Biochemistry and Physiology - Part D:Genomics and Proteomics. ISSN 1744-117X. 50. doi: 10.1016/j.cbd.2024.101233. Fulltekst i vitenarkiv
• Gerber, Lucie Claire Lola; Resseguier, Julien Alain Andre; Helle-Valle, Tellef; Farhat, Elie; Nilsson, Göran Erik & Nilsson, Sjannie Lefevre (2023). Expression of prolyl hydroxylase domains, the upstream regulators of HIF, in the brain of the anoxia-tolerant crucian carp during anoxia-reoxygenation. American Journal of Physiology. Regulatory Integrative and Comparative Physiology. ISSN 0363-6119. 326(2), s. R184–R195. doi: 10.1152/ajpregu.00211.2023. Fulltekst i vitenarkiv Vis sammendrag

  We report a profound upregulation of most egln paralog mRNA levels in anoxia and upon reoxygenation, with egln3ii showing the largest, a 17-fold increase, and highest relative mRNA abundance. The relative abundance of prolyl hydroxylase domain (PHD) proteins was maintained during anoxia and increased at reoxygenation. PHD immunoreactivity was localized to axonal branches with region-specific changes during anoxia-reoxygenation. These dynamic and regional changes in crucian carp, champion of anoxia tolerance, are most likely adaptive and call for further mechanistic studies.

• Farhat, Elie; Talarico, Giancarlo; Grégoire, Mélissa; Weber, Jean-Michel & Mennigen, Jan (2022). Epigenetic and post-transcriptional repression support metabolic suppression in chronically hypoxic goldfish. Scientific Reports. 12. doi: 10.1038/s41598-022-09374-8. Fulltekst i vitenarkiv
• Thoral, Elisa; Farhat, Elie; Roussel, Damien; Cheng, Hang; Guillard, Ludovic & Pamenter, Matthew [Vis alle 8 forfattere av denne artikkelen] (2022). Different patterns of chronic hypoxia lead to hierarchical adaptive mechanisms in goldfish metabolism. Journal of Experimental Biology. ISSN 0022-0949. 225(1). doi: 10.1242/jeb.243194. Fulltekst i vitenarkiv
• Farhat, Elie & Weber, Jean-Michel (2021). Hypometabolic responses to chronic hypoxia: a potential role for membrane lipids. Metabolites. 11(8). doi: 10.3390/metabo11080503. Fulltekst i vitenarkiv
• Farhat, Elie; Cheng, Hang; Romestaing, Caroline; Pamenter, Matthew & Weber, Jean-Michel (2021). Goldfish Response to Chronic Hypoxia: Mitochondrial Respiration, Fuel Preference and Energy Metabolism. Metabolites. 11(3). doi: 10.3390/metabo11030187. Fulltekst i vitenarkiv
• Farhat, Elie; Devereaux, Maiah; Cheng, Hang; Weber, Jean-Michel & Pamenter, Matthew (2021). Na+/K+-ATPase activity is regionally regulated by acute hypoxia in naked mole-rat brain. Neuroscience Letters. ISSN 0304-3940. 764. doi: 10.1016/j.neulet.2021.136244. Fulltekst i vitenarkiv
• Farhat, Elie; Devereaux, Maiah; Pamenter, Matthew & Weber, Jean-Michel (2020). Naked mole-rats suppress energy metabolism and modulate membrane cholesterol in chronic hypoxia. American Journal of Physiology. Regulatory Integrative and Comparative Physiology. ISSN 0363-6119. 319(2), s. R148–R155. doi: 10.1152/ajpregu.00057.2020. Fulltekst i vitenarkiv
• Gatrell, Lauren; Farhat, Elie; Pyle, Glen & Gillis, Todd (2019). Contractile function of the excised hagfish heart during anoxia exposure. Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology. ISSN 0174-1578. 189, s. 199–211. doi: 10.1007/s00360-019-01208-w. Fulltekst i vitenarkiv
• Farhat, Elie; Turenne, Eric; Choi, Kevin & Weber, Jean-Michel (2019). Hypoxia-induced remodelling of goldfish membranes. Comparative Biochemistry and Physiology - Part B: Biochemistry & Molecular Biology. ISSN 1096-4959. 237. doi: 10.1016/j.cbpb.2019.110326. Fulltekst i vitenarkiv

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• Lefevre, Sjannie; Farhat, Elie & Damsgaard, Christian (2025). Spatial transcriptomics reveals region-specific responses to anoxia in the crucian carp brain. Fulltekst i vitenarkiv Vis sammendrag

  Aim The crucian carp (Carassius carassius) is a fish with a remarkable anoxia tolerance, enabling it to survive the Norwegian winters in the wild, where seasonal anoxia is common in small lakes and ponds. While the physiological mechanisms are well documented, there is still a need for better understanding regulation at the molecular and genetic level. The crucian carp brain overall shows a large transcriptional response to anoxia and reoxygenation but given that the brain is a complex and heterogenous tissue, we wanted to explore these transcriptional responses from a spatial perspective. Methods & Results We prepared brain sections from crucian carp that had been exposed to 5 days normoxia and 5 days anoxia, aiming for a transverse plane that would capture the telencephalon, optic tectum (and thalamus). These areas were chosen because previous studies have indicated increased cell death in anoxia and effects on learning and memory, functions associated with the telencephalon, and since it is well known that retinal activity and vision is reduced in anoxia. We used the Visium Spatial Gene Expression system from 10x Genomics to prepare mRNA libraries from the brain sections (four biological samples in each treatment group). Conclusions Based on the mRNA abundance data, we successfully identified brain areas with distinct gene expression patterns: 6 in the telencephalon, 4 in optic tectum, and 2 in thalamus. The study thus confirms the complexity of the crucian carp brain transcriptional response anoxia.

• Gerber, Lucie Claire Lola; Lundeberg, Jenny; Farhat, Elie; Nilsson, Göran Erik & Lefevre, Sjannie (2024). The key to living without oxygen? Succinate transport in the anoxia tolerant crucian carp. Fulltekst i vitenarkiv
• Farhat, Elie; Talarico, Giancarlo; Grégoire, Mélissa; Mennigen, Jan & Weber, Jean-Michel (2022). Epigenetic and post-transcriptional repression supports metabolic suppression in chronically hypoxic goldfish. Fulltekst i vitenarkiv
• Farhat, Elie; Talarico, Giancarlo; Mennigen, Jan & Weber, Jean-Michel (2022). Hypometabolic responses to chronic hypoxia: A role for membrane cholesterol. Fulltekst i vitenarkiv
• Farhat, Elie; Turenne, Eric; Choi, Kevin; Devereaux, Maiah; Pamenter, Matthew & Weber, Jean-Michel (2021). Champions of hypoxia tolerance adjust membrane cholesterol and downregulate metabolism to cope with chronically-low oxygen. Fulltekst i vitenarkiv
• Farhat, Elie; Turenne, Eric; Choi, Kevin & Weber, Jean-Michel (2017). Hypoxia-induced remodelling of goldfish membranes could mediate metabolic suppression. Fulltekst i vitenarkiv

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