Anne Berit C. Samuelsen

• Carvalho, Mariana Mazetto de; Ellefsen, Christiane Færestrand; Eltvik, Andrea Angelov; Hiorth, Marianne & Samuelsen, Anne Berit C. (2025). Chemical structure characterization of polysaccharides using diffusion ordered NMR spectroscopy (DOSY). Carbohydrate Polymers. ISSN 0144-8617. 349(Part B). doi: 10.1016/j.carbpol.2024.123021. Full text in Research Archive
• Carvalho, Mariana Mazetto de; Meyer, Julia; Abahra, Wafa Subhi; Tomic, Ivana; Hiorth, Marianne & Samuelsen, Anne Berit C. (2025). Chemical structure of β-glucans from the mushrooms of Lactarius spp. for development of polysaccharide-coated liposomes. Carbohydrate Polymer Technologies and Applications. 11. doi: 10.1016/j.carpta.2025.100902. Full text in Research Archive
• Carvalho, Mariana Mazetto de; Eltvik, Andrea Angelov; Ellefsen, Christiane Færestrand; Hiorth, Marianne & Samuelsen, Anne Berit C. (2024). Coating of liposomes with β-glucans from the king oyster mushroom (Pleurotus eryngii). Colloids and Surfaces A: Physicochemical and Engineering Aspects. ISSN 0927-7757. 700. doi: 10.1016/j.colsurfa.2024.134783. Full text in Research Archive
• Ellefsen, Christiane Færestrand; Struzek, Anna-Maria; Scherliess, Regina; Hiorth, Marianne & Samuelsen, Anne Berit C. (2023). Preparation of Albatrellus ovinus β-Glucan Microparticles with Dectin-1a Binding Properties. ACS Applied Bio Materials (AABM). 6(5), p. 1863–1872. doi: 10.1021/acsabm.3c00071. Full text in Research Archive Show summary

  Fungal β-glucans are compounds with the potential to activate the innate immune system, in part through binding to the receptor dectin-1. In the present study, small-scale methods for preparing dectin-1a binding microparticles from Albatrellus ovinus alkali-soluble β-glucans were investigated. Mechanical milling was time-consuming and yielded large particles with wide size distributions. Precipitation was more successful: the β-glucan was dissolved in 1 M NaOH, diluted, and precipitated in 1:1 mol equiv HCl. This yielded particles in sizes ranging from 0.5–2 μm. The dectin-1a binding activity was determined using HEK-Blue reporter cells. The prepared particles were able to bind to dectin-1a to the same extent as baker’s-yeast-derived β-glucan particles. The precipitation method was convenient as a quick method for small-scale preparation of β-glucan microparticle dispersions from mushroom β-glucans.

• Ellefsen, Christiane Færestrand; Lindstad, Linda; Klau, Leesa Jane; Aachmann, Finn Lillelund; Hiorth, Marianne & Samuelsen, Anne Berit C. (2023). Investigation of the structural and immunomodulatory properties of alkali-soluble β-glucans from Pleurotus eryngii fruiting bodies. Carbohydrate Polymers. ISSN 0144-8617. 322. doi: 10.1016/j.carbpol.2023.121367. Full text in Research Archive Show summary

  Fungal β-glucans have received a lot of interest due to their proinflammatory activity towards cells of the innate immune system. Although commonly described as (1➔3)-β-glucans with varying degree of (1➔6)-branching, the fungal β-glucans constitute a diverse polysaccharide class. In this study, the alkali-soluble β-glucans from the edible mushroom Pleurotus eryngii were extracted and characterized by GC, GC–MS and 2D NMR analyses. The extracts contain several structurally different polysaccharides, including a (1➔3)-β-d-glucan with single glucose units attached at O-6, and a (1➔6)-β-d-glucan, possibly branched at O-3. The immunomodulatory activities of the P. eryngii extracts were assessed by investigating their ability to bind to the receptor dectin-1, and their ability to induce production of the proinflammatory cytokines TNF-α, IL-6 and IL-1β in LPS-differentiated THP-1 cells. Although the samples were able to bind to the dectin-1a receptor, they did not induce production of significant levels of cytokines in the THP-1 cells. Positive controls of yeast-derived (1➔3)-β-d-glucans with branches at O-6 induced cytokine production in the cells. Thus, it appears that the P. eryngii β-glucans are unable to induce production of proinflammatory cytokines in LPS-differentiated THP-1 cells, despite being able to activate the human dectin-1a receptor.

• Ellefsen, Christiane Færestrand; Wold, Christian Winther; Wilkins, Alistair L.; Rise, Frode & Samuelsen, Anne Berit C. (2021). Water-soluble polysaccharides from Pleurotus eryngii fruiting bodies, their activity and affinity for Toll-like receptor 2 and dectin-1. Carbohydrate Polymers. ISSN 0144-8617. 264. doi: 10.1016/j.carbpol.2021.117991. Full text in Research Archive Show summary

  The mushroom cell wall contains polysaccharides that can activate cells of the innate immune system through receptors such as Toll-like receptors (TLR) and dectin-1. In the present study, Pleurotus eryngii polysaccharide fractions containing a 3-O methylated mannogalactan and (1→3)/(1→6)-β-d-glucans were isolated and extensively characterized by 2D NMR and methylation analysis. Traces of a (1→3)-α-d-glucan and a (1→2)-α-d-mannan were also observed. Affinity for TLR2, TLR2-TLR6 and dectin-1 using HEK-cells expressing the relevant receptor genes was tested. PeWN, containing the 3-O methylated mannogalactan, was inactive towards TLR2, whereas fraction PeWB, containing more β-glucan, activated the TLR2-TLR6 heterodimer. Activation of the human β-glucan receptor dectin-1 correlated with the amount of β-glucan in each fraction. Nitric oxide and cytokine supernatant levels of D2SC/1 dendritic cells stimulated with the P. eryngii fractions and interferon-γ were low to moderate. The results indicate that the immunomodulatory activity of water-soluble P. eryngii polysaccharide fractions is modest.

• Samuelsen, Anne Berit C. (2020). Klaseormedrue (Actaea racemosa L., tidligere Cimicifuga racemosa (L.) Nutt.) ved menopausale plager. Norsk Farmaceutisk Tidsskrift (NFT). ISSN 0029-1935. 7, p. 22–27. Full text in Research Archive
• Samuelsen, Anne Berit C.; Rise, Frode; Wilkins, Alistair L.; Teveleva, Lioubov; Nyman, Anna Armika Tussilago & Aachmann, Finn Lillelund (2019). The edible mushroom Albatrellus ovinus contains a α-L-fuco-α-D-galactan, α-D-glucan, a branched (1→6)-β-D-glucan and a branched (1→3)-β-D-glucan. Carbohydrate Research. ISSN 0008-6215. 471, p. 28–38. doi: 10.1016/j.carres.2018.10.012. Full text in Research Archive Show summary

  Albatrellus ovinus, the sheep polypore, is a large, dense mushroom being rich in cell wall material. Polysaccharides were isolated by sequential extraction, enzymatic treatment and analyzed with respect to monosaccharide composition, glycosidic linkages by methylation and GC-MS as well as NMR spectroscopy. A fucogalactan composed of an (1→6)-α-D-galactan backbone with single α-L-Fucp residues attached at O-2 was identified in the hot water extract obtained after treatment with a protease and size exclusion chromatography. Both the hot water extract and the hot alkali extract contained an (1→4)-α-D-glucan whereas β-D-glucans were mainly present in the latter. Structural analysis suggested the presence of two different β-D-glucan backbone structures; a (1→6)-linked β-D-glucan with single β-D-Glcp residues at O-3 and also a (1→3)-linked β-D-glucan with branches in O-6. In addition there were identified short (1→2)-linked β-D-xylan and (1→3)-α-D-mannan chains.

• Nyman, Anna Armika Tussilago; Aachmann, Finn Lillelund; Rise, Frode; Ballance, Simon & Samuelsen, Anne Berit C. (2016). Structural characterization of a branched (1 → 6)-α-mannan and β-glucans isolated from the fruiting bodies of Cantharellus cibarius. Carbohydrate Polymers. ISSN 0144-8617. 146, p. 197–207. doi: 10.1016/j.carbpol.2016.03.052. Full text in Research Archive Show summary

  A comprehensive characterization of polysaccharides from the chanterelle was performed. Experiments included both linkage analysis by methylation and GC–MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC. A 671 kDa (1 → 6)-linked α-d-mannan with single and short (1 → 2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography. The hot 1 M NaOH extract contained two types of β-glucan; a water soluble (1 → 6)-linked β-d-glucan with single and short (1 → 3)-linked β-glucan side chains (ACcSw) and a less branched (1 → 3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4. Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1 → 6)/(1 → 3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.

• Samuelsen, Anne Berit C. (2016). Oppdagelsen av artemisinin- en Nobelpris verdig. Norsk Farmaceutisk Tidsskrift (NFT). ISSN 0029-1935. 2, p. 33–35. Full text in Research Archive
• Berven, Lise; Karppinen, Pernille Kristensen; Hetland, Geir & Samuelsen, Anne Berit C. (2015). The polar high molecular weight fraction of the agaricus blazei murill extract, andosan™, reduces the activity of the tumor-associated protease, legumain, in RAW 264.7 cells. Journal of Medicinal Food. ISSN 1096-620X. 18(4), p. 429–438. doi: 10.1089/jmf.2014.0018. Full text in Research Archive
• Berven, Lise; Skjeldal, Frode Miltzow; Prydz, Kristian; Zubaidi, Layla Mohammad Khader; Ballance, Simon & Johansen, Harald Thidemann [Show all 7 contributors for this article] (2015). Particulate yeast β-glucan is internalized by RAW 264.7 macrophages and reduces the activity of the tumor-associated protease legumain. Bioactive Carbohydrates and Dietary Fibre. 6(1), p. 15–23. doi: 10.1016/j.bcdf.2015.06.003. Full text in Research Archive
• Samuelsen, Anne Berit C. (2015). Kolesterolsenkende aktivitet av ß-glukan. Norsk Farmaceutisk Tidsskrift (NFT). ISSN 0029-1935. 123(5), p. 25–29. Full text in Research Archive
• Samuelsen, Anne Berit C.; Schrezenmeir, Jürgen & Knutsen, Svein Halvor (2014). Effects of orally administered yeast -derived beta-glucans: A review. Molecular Nutrition & Food Research. ISSN 1613-4125. 58(1), p. 183–193. doi: 10.1002/mnfr.201300338. Full text in Research Archive
• Børud, Bente; Anonsen, Jan Haug; Viburiene, Raimonda; Cohen, Ellen Hanne; Samuelsen, Anne Berit C. & Koomey, Michael (2014). Extended glycan diversity in a bacterial protein glycosylation system linked to allelic polymorphisms and minimal genetic alterations in a glycosyltransferase gene. Molecular Microbiology. ISSN 0950-382X. Volume 94(3), p. 688–699. doi: 10.1111/mmi.12789. Full text in Research Archive Show summary

  Glycans manifest in conjunction with the broad spectrum O-linked protein glycosylation in species within the genus Neisseria display intra- and interstrain diversity. Variability in glycan structure and antigenicity are attributable to differences in the content and expression status of glycan synthesis genes. Given the high degree of standing allelic polymorphisms in these genes, the level of glycan diversity may exceed that currently defined. Here, we identify unique protein-associated disaccharide glycoforms that carry N-acetylglucosamine (GlcNAc) at their non-reducing end. This altered structure was correlated with allelic variants of pglH whose product was previously demonstrated to be responsible for the expression of glucose (Glc)-containing disaccharides. Allele comparisons and site-specific mutagenesis showed that the presence of a single residue, alanine at position 303 in place of a glutamine, was sufficient for GlcNAc versus Glc incorporation. Phylogenetic analyses revealed that GlcNAc-containing disaccharides may be widely distributed within the pgl systems of Neisseria particularly in strains of N. meningitidis. Although analogous minimal structural alterations in glycosyltransferases have been documented in association with lipopolysaccharide and capsular polysaccharide variability, this appears to be the first example in which such changes have been implicated in glycan diversification within a bacterial protein glycosylation system.

• Berven, Lise; Solberg, Rigmor; Truong, Hoa Hong Thi; Arlov, Øystein; Aachmann, Finn Lillelund & Skjåk-Bræk, Gudmund [Show all 9 contributors for this article] (2013). Alginates induce legumain activity in RAW 264.7 cells and accelerate autoactivation of prolegumain. Bioactive Carbohydrates and Dietary Fibre. 2, p. 30–44. doi: 10.1016/j.bcdf.2013.08.003. Full text in Research Archive
• Berven, Lise; Johansen, Harald Thidemann; Solberg, Rigmor; Kolset, Svein Olav & Samuelsen, Anne Berit C. (2013). Autoactivation of prolegumain is accelerated by glycosaminoglycans. Biochimie. ISSN 0300-9084. 95(4), p. 772–781. doi: 10.1016/j.biochi.2012.11.002. Full text in Research Archive Show summary

  The cysteine protease legumain participates in several biological and pathological processes including tumour invasion and metastasis. Legumain is synthesized as a zymogen and undergoes pH-dependent autoactivation of the proform in order to reach an enzymatically active form. Here we demonstrate that the naturally occurring polyanionic glycosaminoglycans (GAGs) chondroitin 4-sulphate (C4S), chondroitin 6-sulphate (C6S), chondroitin 4,6-sulphate (C4,6S), heparin, heparan sulphate (HS) as well as chondroitin sulphate (CS)-derived decasaccharides accelerated the autocatalytic activation of prolegumain through ionic interactions in a concentration-, size- and time-dependent manner at pH 4.0. In contrast, at pH 5.0 only C4S and C4,6S were able to promote prolegumain activation, while CS-derived decasaccharides, C6S, heparin and HS lost their effect at this pH.

• Wangensteen, Helle; Klarpås, Line; Alamgir, Mahiuddin; Samuelsen, Anne Berit C. & Malterud, Karl Egil (2013). Can scientific evidence support using Bangladeshi traditional medicinal plants in the treatment of diarrhoea? A review on seven plants. Nutrients. 5, p. 1757–1800. doi: 10.3390/nu5051757. Full text in Research Archive
• Hetland, Geir; Johnson, Egil; Eide, Dag Markus; Grinde, Bjørn; Samuelsen, Anne Berit C. & Wiker, Harald G (2013). Antimicrobial effects of beta-glucans and pectin and of the Agaricus blazei-based mushroom extract, AndoSanTM. Examples of mouse models for pneumococcal-, fecal bacterial-, and mycobacterial infections. In Mendez-Vilas, A. (Eds.), Microbial pathogens and strategies for combating them: science, technology and education. Formatex Research Center. ISSN 9788494213403. p. 889–898. Full text in Research Archive
• Rieder, Anne & Samuelsen, Anne Berit Calmeyer (2012). Do cereal mixed-linked beta-glucans possess immune-modulating activities? Molecular Nutrition & Food Research. ISSN 1613-4125. 56(4), p. 536–547. doi: 10.1002/mnfr.201100723. Full text in Research Archive
• Smistad, Gro; Bøyum, Silje; Alund, Siv Jorunn; Samuelsen, Anne Berit C. & Hiorth, Marianne (2012). The potential of pectin as a stabilizer for liposomal drug delivery systems. Carbohydrate Polymers. ISSN 0144-8617. 90(3), p. 1337–1344. doi: 10.1016/j.carbpol.2012.07.002. Full text in Research Archive
• Biringanine, Gabriel; Ouedraogo, Moustapha; Vray, Bernhard; Samuelsen, Anne Berit C. & Duez, Pierre (2012). Partial chemical characterization of immunomodulatory polysaccharides from Plantago palmata Hook.f.s. leaves. International Journal of Carbohydrate Chemistry. ISSN 1687-935X. 2012. doi: 10.1155/2012/458456. Full text in Research Archive Show summary

  A previous work on Plantago palmata polysaccharides (PS) attributed immunomodulatory properties of leaves to a polysaccharide fraction (PS50) that stimulated NO and TNF-α production by interferon gamma- (IFN-γ-) activated macrophages. The present work aims to elucidate the chemical structure of these immunomodulatory polysaccharides. Size exclusion chromatography showed that the active polymers present an active fraction with a very high molecular weight (about 1200 kDa). These polysaccharides are pectic in nature, with a predominantly unbranched galacturonan domain and with a domain bearing side chains that consist of highly branched arabinan, galactan, and/or arabinogalactan. Comparatively to the well-known Plantago major biologically active PS, Plantago palmata PS50 contained less arabinogalactan-proteins (AGPs) and had a different composition in glucose, galactose, and galacturonic acid. DNA contamination of the polysaccharide was estimated at about 0.04%, a concentration much lower than those reported immunomodulatory in hyaluronic acid preparations (3 to 15%). Therefore, the eventuality of a contaminating DNA-mediated biological activity could be ruled out.

• Samuelsen, Anne Berit; Rieder, Anne; Grimmer, Stine J Staavi; Michaelsen, Terje Einar & Knutsen, Svein Halvor (2011). Immunomodulatory activity of dietary fiber: arabinoxylan and mixed-linked Beta-glucan isolated from barley show modest activities in vitro. International Journal of Molecular Sciences. ISSN 1661-6596. 12(1), p. 570–587. doi: 10.3390/ijms12010570. Full text in Research Archive
• Wangensteen, Helle; Alamgir, Mahiuddin; Duong, GM; Grønhaug, Tom Erik; Samuelsen, Anne Berit & Malterud, Karl Egil (2010). Chemical and Biological Studies of Medicinal Plants from the Sundarbans Mangrove Forest. In eddouks, mohamed (Eds.), Advances in Phytotherapy Research. Research Signpost. ISSN 9788130802237. p. 59–78. Full text in Research Archive
• Westereng, Bjørge; Coenen, Gerd Jan; Michaelsen, Terje Einar; Voragen, Alphons GJ; Samuelsen, Anne Berit & Schols, Henk A [Show all 8 contributors for this article] (2009). Release and characterization of single side chains of white cabbage pectin and their complement-fixing activity. Molecular Nutrition & Food Research. ISSN 1613-4125. 53(6), p. 780–789. doi: 10.1002/mnfr.200800199. Full text in Research Archive
• Westereng, Bjørge; Coenen, Gerd Jan; Michaelsen, Terje Einar; Samuelsen, Anne Berit; Voragen, Alphons G.J. & Schols, Henk [Show all 7 contributors for this article] (2009). Release and characterization of single side chains of white cabbage pectin and their complement-fixing activity. Molecular Nutrition & Food Research. ISSN 1613-4125. 53(6), p. 780–789. doi: 10.1002/mnfr.200800199. Full text in Research Archive Show summary

  A mixture of single side chains from white cabbage pectin were obtained by anion exchange chromatography after applying mild chemical conditions promoting beta-elimination. These pectin fragments were characterized by their molecular weight distribution, sugar composition, C-13-NMR, and MALDI-TOF-MS analysis. These analyses revealed that the large oligosaccharides released by beta-eliminative treatment were composed of alpha-1,5 linked arabinosyl residues with 2- and 3-linked alpha-arabinosyl side chains, and, or beta-1,4 linked galactosyl side chains. Fractions. were tested for complement-fixing activity in order to determine their interaction with the complement system. These results strongly indicated that there was a minimal unit size responsible for the complement-fixing activity. Neutral pectin fragments (similar to 8 kDa) obtained from beta-elimination were inactive in the complement system, although they contained a sugar composition previously shown to be highly active. Larger pectin fragments (similar to 17 kDa) retained some activity, but much lower than polymers containing rhamnogalacturonan type 1 (RGI) structures isolated from the same source. This implied that structural elements containing multiple side chains is necessary for efficient complement-fixing activity.

• Westereng, B; Michaelsen, Terje Einar; Samuelsen, AB & Knutsen, Svein Halvor (2008). Effects of extraction conditions on the chemical structure and biological activity of white cabbage pectin. Carbohydrate Polymers. ISSN 0144-8617. 72(1), p. 32–42. doi: 10.1016/j.carbpol.2007.07.017. Full text in Research Archive
• Wangensteen, Helle; Alamgir, Mahiuddin; Rajia, S.; Meza, Trine Johansen; Samuelsen, Anne Berit & Malterud, Karl Egil (2007). Cytotoxicity and Brine Shrimp Lethality of Rotenoids and Extracts from Sarcolobus globosus. Natural Product Communications. ISSN 1934-578X. 2(8), p. 841–844. Full text in Research Archive
• Samuelsen, Anne Berit; Westereng, Bjørge; Yousif, Osman; Holtekjølen, Ann Katrin; Michaelsen, Terje Einar & Knutsen, Svein Halvor (2007). Structural features and complement-fixing activity of pectin from three Brassica oleracea varieties: White cabbage, kale, and red kale. Biomacromolecules. ISSN 1525-7797. 8(2), p. 644–649. doi: 10.1021/bm060896l. Full text in Research Archive
• Olennikov, D.N.; Tankhaeva, L.M. & Samuelsen, Anne Berit (2006). Quantitative analysis of polysaccharides from Plantago major leaves using the Dreywood method. Chemistry of Natural Compounds. ISSN 0009-3130. 42(3), p. 265–268. Full text in Research Archive
• Westereng, Bjørge; Yousif, Osman; Michaelsen, Terje Einar; Knutsen, Svein Halvor & Samuelsen, Anne Berit (2006). Pectin isolated from white cabbage - structure and complement-fixing activity. Molecular Nutrition & Food Research. ISSN 1613-4125. 50(8), p. 746–755. doi: 10.1002/mnfr.200600026. Full text in Research Archive Show summary

  This study was done to investigate whether white cabbage contained polysaccharides with immunostimulatory activity using the complement-fixing test as an indicator. The main polysaccharide isolated was of pectin nature. Methanolysis and (13)C-NMR showed that the polymers consisted of highly esterified alpha-galactopyranoside (alpha-GalpA), significant amounts of alpha-arabinose furanoside (alpha-Araf), beta-Galp and lesser amounts of rhamnose in the pyranose form (Rhap) and xylose in the pyranose form (Xylp). Linkage analyses showed that the alpha-GalpA residues were mainly 1,4-linked with small amounts of 1,3,4-linkages. The alpha-Araf residues were mainly terminally (t)- and 1,5-linked, whereas beta-Galp was t-, 1,3-, 1,6-, and 1,3,6-linked. Positive Yariv reaction indicated polymers with arabinogalactan type 2 like structures. alpha-Rhap was mainly present as 1,2- and 1,2,4-linked residues and Xylp was t- and 1,4-linked. The molecular weight varied greatly and was from 10 to 150 kDa. Cabbage polymers had biological activity and this complement-fixing activity was greatly affected by hydrolytic removal of Araf from pectic side chains.

• Wangensteen, Helle; Miron, Anca; Alamgir, Mahiuddin; Rajia, S.; Samuelsen, Anne Berit & Malterud, Karl Egil (2006). Antioxidant and 15-lipoxygenase inhibitory activity of rotenoids, isoflavones and phenolic glycosides from Sarcolobus globosus. Fitoterapia. ISSN 0367-326X. 77, p. 290–295. doi: 10.1016/j.fitote.2006.03.017. Full text in Research Archive
• Wangensteen, Helle; Duong, Gia Man; Alamgir, Mahiuddin; Sarder, Mokadez; Samuelsen, Anne Berit & Malterud, Karl Egil (2006). Biological activities of limonoids, catechins, procyanidins and extracts from Xylocarpus granatum. Natural Product Communications. ISSN 1934-578X. 1(11), p. 985–990. Full text in Research Archive
• Wangensteen, Helle; Alamgir, Mahiuddin; Rajia, S.; Samuelsen, Anne Berit & Malterud, Karl Egil (2005). Rotenoids and isoflavones from Sarcolobus globosus. Planta Medica. ISSN 0032-0943. 71, p. 754–758. Full text in Research Archive
• Olennikov, D.N.; Mikhailova, T.M.; Tankhaeva, L.M. & Samuelsen, Anne Berit (2005). Organic acids of medicinal plants. 1. Plantago major. Chemistry of Natural Compounds. ISSN 0009-3130. 41(4), p. 467–468. Full text in Research Archive

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• Samuelsen, Anne Berit C. (2025). Over halvparten strøk på farmasi ved UiO. [Journal]. Farmatid. Full text in Research Archive
• Samuelsen, Anne Berit C. (2025). Over halvparten strøk i farmasi-faget: -Mange er frustrerte. [Journal]. Aftenposten.no. Full text in Research Archive
• Samuelsen, Anne Berit C. (2025). Farmasi- et kommunikasjonsfag. Full text in Research Archive
• Samuelsen, Anne Berit C. (2024). Tolererer du ikke denne professoren, er det noen yrker du ikke er skikket for. [Journal]. Uniforum. Full text in Research Archive
• Samuelsen, Anne Berit C. (2024). Farmasøytisk institutt får studenter med svake norskkunnskaper. [Journal]. Farmatid. Full text in Research Archive
• Samuelsen, Anne Berit C. (2024). Studenter i helsefag har problemer med norsk: – Vi har ikke ressurser til å drive språkopplæring. [Journal]. Uniforum. Full text in Research Archive
• Carvalho, Mariana Mazetto de; Eltvik, Andrea Angelov; Ellefsen, Christiane Færestrand; Hiorth, Marianne & Samuelsen, Anne Berit C. (2024). β-glucans from the king oyster mushroom (Pleurotus eryngii) as a novel source material for coating liposomes. Full text in Research Archive
• Carvalho, Mariana Mazetto de; Eltvik, Andrea Angelov; Ellefsen, Christiane Færestrand; Hiorth, Marianne & Samuelsen, Anne Berit C. (2023). Liposome surface coating using distinct β-glucans fractions from Pleurotus eryngii. Full text in Research Archive
• Carvalho, Mariana Mazetto de; Eltvik, Andrea Angelov; Hiorth, Marianne & Samuelsen, Anne Berit C. (2023). Development of β-glucans coated liposomes for targeting to the cells of the immune system. Full text in Research Archive
• Mathisen, Georg & Samuelsen, Anne Berit C. (2022). Herring roe will be used in medicine to treat psoriasis. [Internet]. sciencenorway.no. Full text in Research Archive
• Mathisen, Georg & Samuelsen, Anne Berit C. (2022). Silderogn skal bli medisin mot psoriasis. [Internet]. Forskning.no. Full text in Research Archive
• Ellefsen, Christiane Færestrand; Struzek, Anna-Maria; Scherliess, Regina; Hiorth, Marianne & Samuelsen, Anne Berit C. (2022). Mushroom β-glucan microparticles with immuno-activating potential. Full text in Research Archive
• Struzek, Anna-Maria; Ellefsen, Christiane Færestrand; Samuelsen, Anne Berit C. & Scherließ, Regina (2022). Immune activation of different micro- and nanoparticulate materials. Full text in Research Archive
• Klau, Leesa Jane; Aachmann, Finn Lillelund; Samuelsen, Anne Berit C. & Ellefsen, Christiane Færestrand (2021). NMR spectroscopic characterization of water-insoluble glucans from Pleurotus eryngii. Full text in Research Archive
• Ellefsen, Christiane Færestrand; Wold, Christian Winther; Wilkins, Alistair L.; Rise, Frode & Samuelsen, Anne Berit C. (2021). Water-soluble mannogalactan and β-glucans from Pleurotus eryngii fruiting bodies, their activity and affinity for TLR2 and dectin-1 receptors. Full text in Research Archive
• Samuelsen, Anne Berit C. & Lönn-Stensrud, Jessica (2021). Kildehåndtering og kritisk tenkning i det 5-årige masterprogrammet i farmasi. Full text in Research Archive
• Ellefsen, Christiane Færestrand; Hiorth, Marianne & Samuelsen, Anne Berit C. (2021). Preparation of fungal β-glucan particles by acid precipitation. Full text in Research Archive
• Samuelsen, Anne Berit C. (2021). Kommunikasjon og etikk i farmasiutdanningen. Full text in Research Archive
• Samuelsen, Anne Berit C. (2021). Lite seksualitet på farmasiutdanningen. [Journal]. Farmatid.no. Full text in Research Archive
• Samuelsen, Anne Berit C. (2020). Upresist og potensielt helseskadelig om honning fra NRK. Forskersonen.no. Full text in Research Archive
• Samuelsen, Anne Berit C. & Spilde, Ingrid (2020). Våpenet som glapp: Kan betaglukaner du får kjøpt på helsekostbutikken bli et våpen i kampen mot kreft og dødelige infeksjoner? [Internet]. Forskning.no. Full text in Research Archive
• Ellefsen, Christiane Færestrand; Struzek, Anna-Maria; Scherliess, Regina & Samuelsen, Anne Berit C. (2020). Polysaccharide based Particles. Full text in Research Archive
• Ellefsen, Christiane Færestrand & Samuelsen, Anne Berit C. (2019). Isolation and Preliminary Characterization of Polysaccharides from King Oyster Mushroom. Full text in Research Archive
• Samuelsen, Anne Berit C. (2018). Tidløs kolkisin. Veneficus. ISSN 0042-3351. 5, p. 21–23. Full text in Research Archive
• Samuelsen, Anne Berit C. & Nordeng, Hedvig Marie Egeland (2016). De vakreste norske medisinplantene. [Internet]. titan.uio.no. Full text in Research Archive
• Nyman, Anna Armika Tussilago; Aachmann, Finn Lillelund & Samuelsen, Anne Berit C. (2015). Isolation and characterization of polysaccharides from Cantharellus cibarius. Full text in Research Archive
• Knutsen, Svein Halvor; Samuelsen, Anne Berit C.; Moen, Birgitte; Rieder, Anne; Vegarud, Gerd Elisabeth & Måge, Ingrid [Show all 7 contributors for this article] (2015). Fate of arabinoxylan and beta-glucan during simulated digestion by human duodenal and stomach fluids and during in vitro fermentation With human faecal slurry. Full text in Research Archive
• Rud, Ida; Måge, Ingrid; Samuelsen, Anne Berit C.; Moen, Birgitte; Rieder, Anne & Vegarud, Gerd Elisabeth [Show all 7 contributors for this article] (2015). Fate of arabinoxylan and beta-glucan during simulated digestion by human stomach and duodenal fluids and during in vitro fermentation with human faecal slurry. Full text in Research Archive
• Samuelsen, Anne Berit C.; Berven, Lise; Rabani, Helai; Zubaidi, Layla & Skjeldal, Frode Miltzow (2015). Immunomodulating activities of fungal beta-glucans seem superior to those of cereal beta-glucans in vitro. Full text in Research Archive
• Samuelsen, Anne Berit C. (2014). beta-1,3/1,6-glukaner; mulige immunmodulerende substanser. Full text in Research Archive
• Wangensteen, Helle; Klarpås, Line; Alamgir, Mahiuddin; Samuelsen, Anne Berit C. & Malterud, Karl Egil (2014). Medisinplanter fra Bangladesh med tradisjonell bruk i behandling av diaré. BestPractice Gastroenterologi. ISSN 1903-6590. 3(1), p. 15–19. Full text in Research Archive
• Nyman, Anna Armika Tussilago; Aas, Hoai Thi Nguyen; Aachmann, Finn Lillelund; Rieder, Anne & Samuelsen, Anne Berit C. (2014). Isolation and characterization of glucans in Cantharellus cibarius. Planta Medica. ISSN 0032-0943. 80(16), p. 1399–1399. doi: 10.1055/s-0034-1394621. Full text in Research Archive
• Berven, Lise; Solberg, Rigmor & Samuelsen, Anne Berit C. (2014). Effects of alginates on legumain Activity in RAW 264.7 cells and on prolegumain autoactivation. Planta Medica. ISSN 0032-0943. 80(16), p. 1358–1358. doi: 10.1055/s-0034-1394502. Full text in Research Archive
• Rieder, Anne; Knutsen, Svein Halvor; Ballance, Simon & Samuelsen, Anne Berit C. (2014). Fiberseminar. Full text in Research Archive
• Knutsen, Svein Halvor; Moen, Birgitte; Rud, Ida; Vegarud, Gerd Elisabeth; Grimmer, Stine J Staavi & Rieder, Anne [Show all 7 contributors for this article] (2013). In vitro based methodology to study potential health effects of arabinoxylan and beta-glucan. Full text in Research Archive
• Samuelsen, Anne Berit C. (2013). Plantebaserte produkter: Inn i varmen og kommet for å bli. Norsk Farmaceutisk Tidsskrift (NFT). ISSN 0029-1935. 7-8, p. 18–18. Full text in Research Archive
• Samuelsen, Anne Berit C. (2013). Planter som ugress, mat og medisin. Full text in Research Archive
• Samuelsen, Anne Berit C. (2012). Internasjonal plantedag på Blindern. Norsk Farmaceutisk Tidsskrift (NFT). ISSN 0029-1935. 6, p. 37–37. Full text in Research Archive
• Samuelsen, Anne Berit Calmeyer (2012). Medisinplanter i Urtehagen. Full text in Research Archive
• Berven, Lise; Johansen, Harald Thidemann; Solberg, Rigmor; Kolset, Svein Olav & Samuelsen, Anne Berit C. (2012). INTERACTIONS BETWEEN PROLEGUMAIN AND GLYCOSAMINOGLYCANS. Full text in Research Archive
• Samuelsen, Anne Berit C. (2012). Fra plante til medisin. Full text in Research Archive Show summary

  Planter er opprinnelsen til dagens medisin, og fortsatt er kjemiske forbindelser isolert fra planter vesentlige i utviklingen av nye legemidler. Disse plantene kan være hentet fra tradisjonell bruk, men noen er også oppdaget mer tilfeldig.

• Knutsen, Svein H. & Samuelsen, Anne Berit (2011). Beta-glukaner fra kornvarer kan gi flere positive helsegevinster. Baker og Konditor. ISSN 0803-253X. 4, p. 30–31. Full text in Research Archive
• Samuelsen, Anne Berit Calmeyer (2011). Medisinplanter: eksempler fra folkemedisinsk bruk til legemidler. Full text in Research Archive
• Samuelsen, Anne Berit Calmeyer (2011). Pharmacognosy`s place in the 5-year integrated Master`s degree programme at University of Oslo. Full text in Research Archive
• Samuelsen, Anne Berit Calmeyer; Rieder, Anne; Grimmer, Stine; Michaelsen, Terje E. & Knutsen, Svein H. (2011). Dietary fibre and health: The activities of barley beta-glucan and arabinoxylan in selected test systems. Full text in Research Archive
• Samuelsen, Anne Berit; Grimmer, Stine J Staavi; Michaelsen, Terje Einar; Rieder, Anne; Jensen, Merethe & Knutsen, Svein Halvor (2010). Barley arabinoxylan and mixed linked beta-glucan exhibit low responses in selected in vitro test systems. Full text in Research Archive
• Alamgir, Mahiuddin; Wangensteen, Helle; Uddin, S.J.; Samuelsen, Anne Berit & Malterud, Karl Egil (2007). Chemical and biological investigation of medicinal plants of the Sundarbans mangrove forest. Full text in Research Archive
• Samuelsen, Anne Berit (2007). Helse i hvert et kålblad. Immunologisk aktivitet av pektin. Full text in Research Archive
• Wangensteen, Helle; Alamgir, Mahiuddin; Samuelsen, Anne Berit & Malterud, Karl Egil (2007). Adenosine deaminase inhibitors from Bangladeshi medicinal plants. Full text in Research Archive
• Wangensteen, Helle; Duong, Gia Man; Alamgir, Mahiuddin; Sarder, Mokadez; Samuelsen, Anne Berit & Malterud, Karl Egil (2006). Antioxidants from Xylocarpus granatum. Full text in Research Archive
• Samuelsen, Anne Berit (2006). Groblad og sårheling. Full text in Research Archive
• Samuelsen, Anne Berit; Yousif, Osman; Westereng, Bjørge; Michaelsen, Terje Einar & Knutsen, Svein Halvor (2006). Bioactive pectin fractions in white cabbage (Brassica oleracea var. capitata). Full text in Research Archive
• Wangensteen, Helle; Duong, Gia Man; Alamgir, Mahiuddin; Sarder, M.; Samuelsen, Anne Berit & Malterud, Karl Egil (2006). Antioxidants from Xylocarpus granatum. Abstracts, nr. P115. Full text in Research Archive
• Samuelsen, Anne Berit; Michaelsen, Terje Einar; Meza, Trine Johansen; Holtekjølen, Ann Katrin; Sahlstrøm, Stephan & Knutsen, Svein Halvor (2006). Studies on immunomodulatory activity of barley polysaccharides. Full text in Research Archive
• Wangensteen, Helle; Alamgir, Mahiuddin; Rajia, S.; Samuelsen, Anne Berit & Malterud, Karl Egil (2005). Sarcolobin and sarcolobone - new isoflavonoids from Sarcolobus globosus. Full text in Research Archive
• Wangensteen, Helle; Alamgir, Mahiuddin; Rajia, S.; Samuelsen, Anne Berit & Malterud, Karl Egil (2005). Sarcolobin og sarcolobine - nye kjemiske substanser identifisert i liane fra mangroveskogen i Bangladesh. Full text in Research Archive
• Wangensteen, Helle; Alamgir, Mahiuddin; Rajia, S.; Samuelsen, Anne Berit & Malterud, Karl Egil (2005). Sarcolobin and sarcolobone - New isoflavonoids from Sarcolobus globosus. Full text in Research Archive
• Samuelsen, Anne Berit (2005). Plantago major L. "Healing leaves" that contain bioactive pectin type polysaccharides. Full text in Research Archive
• Samuelsen, Anne Berit; Yousif, Osman; Knutsen, Svein H.; Westereng, Bjørge; Michaelsen, Terje E. & Bråthen, Erland (2005). Bioactive polysaccharides are present in white cabbage, a plant used both as food and medicine. Full text in Research Archive
• Heinicke, Fatima; Rieder, Anne; Samuelsen, Anne Berit C. & Knutsen, Svein Halvor (2015). Establishing a gene expression system to screen the effects of dietary fibers and their metabolites on selected aspects of colon cancer development. Universitetet i Oslo. Full text in Research Archive
• Fossum, Gro; Saboni, Mitra; Samuelsen, Anne Berit C. & Nordeng, Hedvig Marie Egeland (2014). Assessment report on Rubus idaeus L., folium. European Medicines Agency. Full text in Research Archive
• Fossum, Gro; Saboni, Mitra & Samuelsen, Anne Berit C. (2014). Assessment report on Sambucus nigra L., fructus. European Medicines Agency. Full text in Research Archive
• Sabouni, Mitra; Fossum, Gro; Samuelsen, Anne Berit C. & Nordeng, Hedvig Marie Egeland (2012). Felleskapsmonografier Bringebærblad og Svarthyllbær. Universitetet i Oslo. Full text in Research Archive Show summary

  Bakgrunn EUs vitenskapelige komité for plantebaserte legemidler (HMPC- Herbal Medicinal Products Committee) sin hovedoppgave er å utarbeide monografier. Utarbeidelse av monografier blir utført av medlemmer av MLWP (Working Party on samfunn monografier og samfunn List) som rapportører og deres tilgjengelige eksperter. MLWP er en arbeidsgruppe under HMPC. Fellesskapsmonografiene gir en harmonisert tilnærming til vitenskapelig bedømmelse av plantebaserte legemidler i EU/EØS-området, og kan legges til grunn ved vurdering av søknad om markedsføringstillatelse for plantebaserte legemidler. I samarbeid med Farmasøytisk institutt ved Universitetet i Oslo, besluttet Statens legemiddelverk å lage monografier for svarthyllbær og bringebærblad. Resultatet av dette samarbeidet er denne oppgaven. Hensikt Formålet med oppgaven er å utarbeide fellesskapsmonografier og utredningsrapporter (”Assessment reports”) som gir oversikt over sikkerhet - og effektdokumentasjon for de aktuelle plantedrogene, samt litteraturgjennomgang. Metode Retningslinjer og maler fra HMPC er lagt til grunn for bruk av litteratur ved utarbeidelse av monografiene og utredningsrapportene. Håndbøker, vitenskapelige artikler, tilgjengelige farmakopéer er benyttet i oppgaven. I tillegg er vanlig søkestrategier i vitenskapelige databaser med avgrensing av søkeord og kombinerte søk, brukt. Resultater og diskusjon Oppgaven omfatter to monografier, utredningsrapporter og referanselister for bringebærblad og svarthyllbær. Disse urtene er tradisjonelt brukt ved ulike indikasjoner. Monografiene og utredningsrapportene har vært gjennom en serie revideringer i MLWP etter tilbakemeldinger fra diskusjoner i arbeidsgruppen under møtene. HMPC står ansvarlig for godkjenning av alle monografier og utredningsrapporter. Tilbakemeldinger fra diskusjoner i MLWP er tatt hensyn til i denne prosessen. Bringebærblad har blitt diskutert fem ganger i MLWP og vil bli diskutert igjen i januar 2013. MLWP skal avklare om monografien kan sendes til HMPC. HMPC avgjør i mars 2013 om den kan publiseres for høring hos interesserte parter. Svarthyllbær dokumentene sendes til HMPC i januar 2013 slik at de kan vedta at den kan legges ut til høring. Etter høringen vil kommentarer fra interesserte parter bli diskutert. Hvis endringer anses som nødvendig, vil dokumentene bli revidert, og HMPC vil vedta monografien. Når monografiene er endelig vedtatt av HMPC publiseres de på EMAs nettsider for godkjente monografier. Ettersom bringebærblad både har en lang brukstradisjon og fortsatt er i bruk som fødselsforberedende middel, ble det likevel ikke inkludert som en mulig indikasjon i monografien. Mangel på sikkerhetsdata som oppfyller kravene for bruk under svangerskapet som gjelder ved godkjenning av legemidler, gjør at denne indikasjonen ikke kan anbefales av arbeidsgruppen. Arbeidsgruppen forventer kommentarer fra interesserte parter på dette etter høringen, og eventuelle kommentarer vil besvares etter høringsperioden i et eget dokument. En mulig forkjølelsesindikasjon for svarthyllbær har vært mye diskutert. Til tross for at svarthyllbær er populært som forkjølelsesmiddel, har dokumentsjonen ikke blitt vurdert til å kunne oppfylle kravene til tradisjonell bruk. Dersom det kommer inn dokumentasjon som viser 30 års bruk i løpet av høringsperioden fra interesserte parter, kan dette endre seg. Det kan også komme inn kommentarer som endrer den nåværende foreslåtte indikasjonen, dosering eller annen informasjon i dokumentene som blir publisert for høring for svarthyllbær. Konklusjon Når monografiene for svarthyllbær og bringebærblad er endelig godkjent, vil de omfatte plantebaserte tilberedninger som kan vurderes som sikre når de brukes i anbefalte doser og de angitte betingelsene som er beskrevet i monografiene. Felleskapsmonografier skal brukes til å forenkle godkjenningsprosessen og forbedre produktinformasjonen for plantebaserte legemidler i EU/EØS-land. Universitetet i Oslo har i samarbeid med Statens legemiddelverk på bakgrunn av dette arbeidet, bidratt til å utarbeide to monografier som inngår i denne hovedfagsoppgaven. Disse monografiene vil kunne brukes i godkjenningen av tradisjonelle plantebaserte legemidler i EU/EØS-land.

• Samuelsen, Anne Berit C.; Berven, Lise; Solberg, Rigmor; Johansen, Harald Thidemann & Truong, Hong Hoa Thi (2012). Effekt av alginat på legumain, en protease involvert i kreftutvikling. Universitetet i Oslo. Full text in Research Archive Show summary

  Bakgrunn: Alginat er en kjent polymer som har vært mye utnyttet kommersielt, men også i legemiddelbransjen de siste årene. Alginat er et løselig kostfiber som gir immunrespons ved cellestimulering. Mange studier har vist en tendens til at kosthold med mye kostfiber gir lavere insidens tykktarms-endetarmskreft. En tidligere masteroppgave ved forskningsgruppen har vist at legumainaktivitet ble påvirket av alginat ved in vitro eksperimenter, både ved direkte autoaktivering prolegumain og ved stimulering av makrofagceller. Denne oppgaven bygget videre på dette og effekten av alginat på legumain ble nærmere studert, der legumain er en cysteinprotease som har vist relasjon til kreftutvikling. Metoder: Det ble brukt forskjellige metoder for å karakterisere alginat M-60 med innhold av 60 % mannuronsyre og M-100 med 100 % mannuronsyre. Monosakkaridsammensetningen til disse alginatene ble bestemt ved metanolyse, GC, og NMR og de ble også redusert for å bestemme ladningenes betydning. Det ble utviklet en metode for å fremstille så ren alginatoligomere som mulig, ved anvendelse av alginatlyase for å degradere alginatet og deretter fraksjonering av prøvene på ionebytterkolonnen. Fraksjonene ble videre analysert på HPLC, HPAEC-PAD og MS for å bestemme størrelsen til oligomerene. Ikke redusert- og redusert M-60 og M-100, alginatlyasebehandlet M-60, og i tillegg M-75 og M-94 ble videre testet for evne til å påvirke legumainaktivitet. To ulike forhold ble studert; Stimulering av makrofager (RAW 246.7-celler) i kultur eller direkte inkubering av prolegumain sammen med de ulike alginatprøvene for å undersøke deres evne til å stimulere autoaktivering av prolegumain. Resultat: NMR-metode gav en mer nøyaktig bestemmelse av monosakkaridsammensetning til alginat i forhold til GC-metoden. Alginatdegraderingsprosessen ved behandling med alginatlyasen ble vellykket med i både buffer og vann, og vann ble derfor benyttet for å slippe å gjøre desalting. Ved direkte autoaktivering prolegumain gav de ubehandlede alginatene M-60, M-75, M-94 og M-100 øking i legumainaktiviteten. Alginat M-100 og M-94 gav mest stimulering av prolegumain autoaktivering med 7 ganger høyere legumainaktiviteten enn kontroll, mens M-60 gav 6 ganger høyere og M-75 gav 5 ganger høyere legumainaktivitet. Reduserte M-100 ga ingen stimulering av legumain autoaktivering, mens redusert M-60 og M-60 ikke fjernet borat gav 5 ganger høyere legumainaktivitet. Sammenlignet med kontrollen gav lyasebehandlet M-60-fraksjoner B2 og C4 ingen effekt, mens fraksjon B3 gav 20 % høyere og fraksjon C5 gav 40 % høyere legumainaktivitet ved autoaktiveringsforsøk. I celleforsøk gav ubehandlet alginat M-60 veldig varierende resultater. I to forsøk stimulerte den økning legumainaktivitet, men i et forsøk gjorde den ikke. M-75 og M-94 gav en hemmende effekt, der M-75 gav 50 % og M-94 gav 90 % lavere legumainaktivitet enn kontroll. M-100 gav ingen effekt. Reduserte M-60 og M-100 og de lyasebehandlede-M-60-fraksjonene gav en hemmende effekt av legumainaktivitet. Redusert M-100 gav 30 % mindre legumainaktivitet enn kontroll, men M-60 gav 50 % mindre aktivitet. Lyasebehandlet M-60- fraksjon gav 10-30 % lavere legumainaktivitet enn kontroll.

• Samuelsen, Anne Berit C.; Berven, Lise; Solberg, Rigmor; Johansen, Harald Thidemann & Nguyen, Nga Thi (2011). Studier av alginats effekt på prolegumain og cellesekresjon av matriksmetalloproteaser. Universitetet i Bergen. Full text in Research Archive

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