Anne Berit C. Samuelsen

• Carvalho, Mariana Mazetto de; Ellefsen, Christiane Færestrand; Eltvik, Andrea Angelov; Hiorth, Marianne & Samuelsen, Anne Berit C. (2025). Chemical structure characterization of polysaccharides using diffusion ordered NMR spectroscopy (DOSY). Carbohydrate Polymers. ISSN 0144-8617. 349(Part B). doi: 10.1016/j.carbpol.2024.123021. Fulltekst i vitenarkiv
• Carvalho, Mariana Mazetto de; Meyer, Julia; Abahra, Wafa Subhi; Tomic, Ivana; Hiorth, Marianne & Samuelsen, Anne Berit C. (2025). Chemical structure of β-glucans from the mushrooms of Lactarius spp. for development of polysaccharide-coated liposomes. Carbohydrate Polymer Technologies and Applications. 11. doi: 10.1016/j.carpta.2025.100902. Fulltekst i vitenarkiv
• Carvalho, Mariana Mazetto de; Eltvik, Andrea Angelov; Ellefsen, Christiane Færestrand; Hiorth, Marianne & Samuelsen, Anne Berit C. (2024). Coating of liposomes with β-glucans from the king oyster mushroom (Pleurotus eryngii). Colloids and Surfaces A: Physicochemical and Engineering Aspects. ISSN 0927-7757. 700. doi: 10.1016/j.colsurfa.2024.134783. Fulltekst i vitenarkiv
• Ellefsen, Christiane Færestrand; Struzek, Anna-Maria; Scherliess, Regina; Hiorth, Marianne & Samuelsen, Anne Berit C. (2023). Preparation of Albatrellus ovinus β-Glucan Microparticles with Dectin-1a Binding Properties. ACS Applied Bio Materials (AABM). 6(5), s. 1863–1872. doi: 10.1021/acsabm.3c00071. Fulltekst i vitenarkiv Vis sammendrag

  Fungal β-glucans are compounds with the potential to activate the innate immune system, in part through binding to the receptor dectin-1. In the present study, small-scale methods for preparing dectin-1a binding microparticles from Albatrellus ovinus alkali-soluble β-glucans were investigated. Mechanical milling was time-consuming and yielded large particles with wide size distributions. Precipitation was more successful: the β-glucan was dissolved in 1 M NaOH, diluted, and precipitated in 1:1 mol equiv HCl. This yielded particles in sizes ranging from 0.5–2 μm. The dectin-1a binding activity was determined using HEK-Blue reporter cells. The prepared particles were able to bind to dectin-1a to the same extent as baker’s-yeast-derived β-glucan particles. The precipitation method was convenient as a quick method for small-scale preparation of β-glucan microparticle dispersions from mushroom β-glucans.

• Ellefsen, Christiane Færestrand; Lindstad, Linda; Klau, Leesa Jane; Aachmann, Finn Lillelund; Hiorth, Marianne & Samuelsen, Anne Berit C. (2023). Investigation of the structural and immunomodulatory properties of alkali-soluble β-glucans from Pleurotus eryngii fruiting bodies. Carbohydrate Polymers. ISSN 0144-8617. 322. doi: 10.1016/j.carbpol.2023.121367. Fulltekst i vitenarkiv Vis sammendrag

  Fungal β-glucans have received a lot of interest due to their proinflammatory activity towards cells of the innate immune system. Although commonly described as (1➔3)-β-glucans with varying degree of (1➔6)-branching, the fungal β-glucans constitute a diverse polysaccharide class. In this study, the alkali-soluble β-glucans from the edible mushroom Pleurotus eryngii were extracted and characterized by GC, GC–MS and 2D NMR analyses. The extracts contain several structurally different polysaccharides, including a (1➔3)-β-d-glucan with single glucose units attached at O-6, and a (1➔6)-β-d-glucan, possibly branched at O-3. The immunomodulatory activities of the P. eryngii extracts were assessed by investigating their ability to bind to the receptor dectin-1, and their ability to induce production of the proinflammatory cytokines TNF-α, IL-6 and IL-1β in LPS-differentiated THP-1 cells. Although the samples were able to bind to the dectin-1a receptor, they did not induce production of significant levels of cytokines in the THP-1 cells. Positive controls of yeast-derived (1➔3)-β-d-glucans with branches at O-6 induced cytokine production in the cells. Thus, it appears that the P. eryngii β-glucans are unable to induce production of proinflammatory cytokines in LPS-differentiated THP-1 cells, despite being able to activate the human dectin-1a receptor.

• Ellefsen, Christiane Færestrand; Wold, Christian Winther; Wilkins, Alistair L.; Rise, Frode & Samuelsen, Anne Berit C. (2021). Water-soluble polysaccharides from Pleurotus eryngii fruiting bodies, their activity and affinity for Toll-like receptor 2 and dectin-1. Carbohydrate Polymers. ISSN 0144-8617. 264. doi: 10.1016/j.carbpol.2021.117991. Fulltekst i vitenarkiv Vis sammendrag

  The mushroom cell wall contains polysaccharides that can activate cells of the innate immune system through receptors such as Toll-like receptors (TLR) and dectin-1. In the present study, Pleurotus eryngii polysaccharide fractions containing a 3-O methylated mannogalactan and (1→3)/(1→6)-β-d-glucans were isolated and extensively characterized by 2D NMR and methylation analysis. Traces of a (1→3)-α-d-glucan and a (1→2)-α-d-mannan were also observed. Affinity for TLR2, TLR2-TLR6 and dectin-1 using HEK-cells expressing the relevant receptor genes was tested. PeWN, containing the 3-O methylated mannogalactan, was inactive towards TLR2, whereas fraction PeWB, containing more β-glucan, activated the TLR2-TLR6 heterodimer. Activation of the human β-glucan receptor dectin-1 correlated with the amount of β-glucan in each fraction. Nitric oxide and cytokine supernatant levels of D2SC/1 dendritic cells stimulated with the P. eryngii fractions and interferon-γ were low to moderate. The results indicate that the immunomodulatory activity of water-soluble P. eryngii polysaccharide fractions is modest.

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• Samuelsen, Anne Berit C. (2025). Over halvparten strøk på farmasi ved UiO. [Journal]. Farmatid. Fulltekst i vitenarkiv
• Samuelsen, Anne Berit C. (2025). Over halvparten strøk i farmasi-faget: -Mange er frustrerte. [Journal]. Aftenposten.no. Fulltekst i vitenarkiv
• Samuelsen, Anne Berit C. (2025). Farmasi- et kommunikasjonsfag. Fulltekst i vitenarkiv
• Samuelsen, Anne Berit C. (2024). Tolererer du ikke denne professoren, er det noen yrker du ikke er skikket for. [Journal]. Uniforum. Fulltekst i vitenarkiv
• Samuelsen, Anne Berit C. (2024). Farmasøytisk institutt får studenter med svake norskkunnskaper. [Journal]. Farmatid. Fulltekst i vitenarkiv
• Samuelsen, Anne Berit C. (2024). Studenter i helsefag har problemer med norsk: – Vi har ikke ressurser til å drive språkopplæring. [Journal]. Uniforum. Fulltekst i vitenarkiv
• Carvalho, Mariana Mazetto de; Eltvik, Andrea Angelov; Ellefsen, Christiane Færestrand; Hiorth, Marianne & Samuelsen, Anne Berit C. (2024). β-glucans from the king oyster mushroom (Pleurotus eryngii) as a novel source material for coating liposomes. Fulltekst i vitenarkiv
• Carvalho, Mariana Mazetto de; Eltvik, Andrea Angelov; Ellefsen, Christiane Færestrand; Hiorth, Marianne & Samuelsen, Anne Berit C. (2023). Liposome surface coating using distinct β-glucans fractions from Pleurotus eryngii. Fulltekst i vitenarkiv
• Carvalho, Mariana Mazetto de; Eltvik, Andrea Angelov; Hiorth, Marianne & Samuelsen, Anne Berit C. (2023). Development of β-glucans coated liposomes for targeting to the cells of the immune system. Fulltekst i vitenarkiv
• Mathisen, Georg & Samuelsen, Anne Berit C. (2022). Herring roe will be used in medicine to treat psoriasis. [Internet]. sciencenorway.no. Fulltekst i vitenarkiv
• Mathisen, Georg & Samuelsen, Anne Berit C. (2022). Silderogn skal bli medisin mot psoriasis. [Internet]. Forskning.no. Fulltekst i vitenarkiv
• Ellefsen, Christiane Færestrand; Struzek, Anna-Maria; Scherliess, Regina; Hiorth, Marianne & Samuelsen, Anne Berit C. (2022). Mushroom β-glucan microparticles with immuno-activating potential. Fulltekst i vitenarkiv
• Struzek, Anna-Maria; Ellefsen, Christiane Færestrand; Samuelsen, Anne Berit C. & Scherließ, Regina (2022). Immune activation of different micro- and nanoparticulate materials. Fulltekst i vitenarkiv
• Klau, Leesa Jane; Aachmann, Finn Lillelund; Samuelsen, Anne Berit C. & Ellefsen, Christiane Færestrand (2021). NMR spectroscopic characterization of water-insoluble glucans from Pleurotus eryngii. Fulltekst i vitenarkiv

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