chunlins

• Tu, Yanyun; Cheng, Lina; Liu, Xianfeng; Hammerstad, Marta; Shi, Chunlin & Meng, Sida [Vis alle 9 forfattere av denne artikkelen] (2025). SlIDL6–SlHSL1/2/3 ligand-receptor pairs regulate tomato pedicel abscission. Journal of Integrative Agriculture (JIA). ISSN 2095-3119. 25(1), s. 118–126. doi: 10.1016/j.jia.2025.03.018. Fulltekst i vitenarkiv Vis sammendrag

  Flower and fruit abscission reduce crop yield, so decreasing abscission is a significant agricultural issue. HAESA (HAE) and HAESA-like2 (HSL2) kinases and their ligand, INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) peptide, have been confirmed to be the core elements regulating floral organ abscission in Arabidopsis thaliana. Our earlier research revealed that SlIDL6, a homolog of IDA in tomato, functions similarly to AtIDA, regulating the abscission of tomato flower organs. Here, we further isolated three HAESA-like homologs, SlHSL1/2/3, which are involved in tomato flower abscission. SlHSL1/2/3 are highly expressed in the abscission zone (AZ). The knockout mutant lines of Slhsl1, Slhsl2, and Slhsl3 showed lower flower pedicel abscission than wild type (WT). The double mutant of Slhsl1Slhsl2, Slhsl1Slhsl3, and Slhsl2Slhsl3 further depressed abscission than each of the single mutant lines, while triple mutants Slhsl1Slhsl2Slhsl3 exhibited the lowest abscission, indicating that SlHSL1/2/3 mediated abscission is non-redundancy, at least partially. Treating tomato pedicel explants with SlIDL6 peptide significantly accelerated pedicel abscission in WT. However, it had little effect on the abscission rate of SlHSL1/2/3 knockout lines, indicating that SlHSL1/2/3 are the receptors of SlIDL6 in pedicel abscission. Ethylene action inhibitor 1-methylcyclopropene (1-MCP) can significantly depress the expression of SlHSL1/2/3. Ethylene can significantly accelerate the abscission of WT, while less abscission was found in SlHSL1/2/3 knockout lines. Our findings indicate that SlHSL1/2/3 can act as receptors for SlIDL6 to positively regulate tomato pedicel abscission, and the abscission regulated by SlHSL1/2/3 was partially dependent on ethylene.

• Li, Ruizhen; Shi, Chunlin; Wang, Xiaoyang; Meng, Yan; Cheng, Lina & Jiang, Cai-Zhong [Vis alle 9 forfattere av denne artikkelen] (2021). Inflorescence abscission protein SlIDL6 promotes low light intensity-induced tomato flower abscission. Plant Physiology. ISSN 0032-0889. 186(2), s. 1288–1301. doi: 10.1093/PLPHYS/KIAB121. Fulltekst i vitenarkiv
• Zhao, Minglei; Li, Caiqin; Ma, Xingshuai; Xia, Rui; Chen, Jianye & Liu, Xuncheng [Vis alle 11 forfattere av denne artikkelen] (2020). KNOX protein KNAT1 regulates fruitlet abscission in litchi by repressing ethylene biosynthetic genes. Journal of Experimental Botany. ISSN 0022-0957. 71(14), s. 4069–4082. doi: 10.1093/jxb/eraa162. Fulltekst i vitenarkiv
• Wang, Rong; Shi, Chunlin; Wang, Xiaoyang; Li, Ruizhen; Meng, Yan & Cheng, Lina [Vis alle 9 forfattere av denne artikkelen] (2020). Tomato SlIDA has a critical role in tomato fertilization by modifying reactive oxygen species homeostasis. The Plant Journal. ISSN 0960-7412. 103(6), s. 2100–2118. doi: 10.1111/tpj.14886. Fulltekst i vitenarkiv
• Shi, Chunlin; Alling, Renate; Hammerstad, Marta & Aalen, Reidunn B. (2019). Control of organ abscission and other cell separation processes by evolutionary conserved peptide signaling. Plants. 8(7), s. 1–15. doi: 10.3390/plants8070225. Fulltekst i vitenarkiv Vis sammendrag

  Plants both generate and shed organs throughout their lifetime. Cell separation is in function during opening of anthers to release pollen; floral organs are detached after pollination when they have served their purpose; unfertilized flowers are shed; fruits and seeds are abscised from the mother plant to secure the propagation of new generations. Organ abscission takes place in specialized abscission zone (AZ) cells where the middle lamella between adjacent cell files is broken down. The plant hormone ethylene has a well-documented promoting effect on abscission, but mutation in ethylene receptor genes in Arabidopsis thaliana only delays the abscission process. Microarray and RNA sequencing have identified a large number of genes differentially expressed in the AZs, especially genes encoding enzymes involved in cell wall remodelling and disassembly. Mutations in such genes rarely give a phenotype, most likely due to functional redundancy. In contrast, mutation in the INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) blocks floral organ abscission in Arabidopsis. IDA encodes a small peptide that signals through the leucine-rich repeat receptor-like kinases HAESA (HAE) and HAE-LIKE2 (HSL2) to control floral organ abscission and facilitate lateral root emergence. Untimely abscission is a severe problem in many crops, and in a more applied perspective, it is of interest to investigate whether IDA-HAE/HSL2 is involved in other cell separation processes and other species. Genes encoding IDA and HSL2 orthologues have been identified in all orders of flowering plants. Angiosperms have had enormous success, with species adapted to all kinds of environments, adaptations which include variation with respect to which organs they shed. Here we review, from an evolutionary perspective, the properties of the IDA-HAE/HSL2 signaling module and the evidence for its hypothesized involvement in various cell separation processes in angiosperms.

• Shi, Chunlin & Butenko, Melinka Alonso (2018). Quantitative Analysis of Floral Organ Abscission in Arabidopsis Via a Petal Breakstrength Assay. I Guo, Yongfeng (Red.), Plant Senescence: Methods and Protocols (Methods in Molecular Biology). Humana Press. ISSN 9781493976720. s. 81–88. doi: 10.1007/978-1-4939-7672-0_6. Fulltekst i vitenarkiv
• Shi, Chunlin; Wangenheim, Daniel von; Herrmann, Ullrich; Wildhagen, Mari; Kulik, Ivan & Kopf, Andreas [Vis alle 16 forfattere av denne artikkelen] (2018). The dynamics of root cap sloughing in Arabidopsis is regulated by peptide signalling. Nature Plants. ISSN 2055-026X. 4(8), s. 596–604. doi: 10.1038/s41477-018-0212-z. Fulltekst i vitenarkiv
• Shi, Chunlin & Butenko, Melinka Alonso (2018). Visualizing Morphological Changes of Abscission Zone Cells in Arabidopsis by Scanning Electron Microscope. I Guo, Yongfeng (Red.), Plant Senescence: Methods and Protocols (Methods in Molecular Biology). Humana Press. ISSN 9781493976720. s. 321–337. doi: 10.1007/978-1-4939-7672-0_26. Fulltekst i vitenarkiv
• Czyzewicz, Nathan; Shi, Chunlin; Vu, Lam Dai; Cotte, Brigitte Van De; Hodgman, Charlie & Butenko, Melinka Alonso [Vis alle 7 forfattere av denne artikkelen] (2015). Modulation of Arabidopsis and monocot root architecture by CLAVATA3/EMBRYO SURROUNDING REGION 26 peptide. Journal of Experimental Botany. ISSN 0022-0957. 66(17), s. 5229–5243. doi: 10.1093/jxb/erv360. Fulltekst i vitenarkiv
• Liu, Bin; Butenko, Melinka Alonso; Shi, Chunlin; Bolivar, Jenny; Winge, Per & Stenvik, Grethe-Elisabeth [Vis alle 14 forfattere av denne artikkelen] (2013). NEVERSHED and INFLORESCENCE DEFICIENT IN ABSCISSION are differentially required for cell expansion and cell separation during floral organ abscission in Arabidopsis thaliana. Journal of Experimental Botany. ISSN 0022-0957. 64(17), s. 5345–5357. doi: 10.1093/jxb/ert232. Fulltekst i vitenarkiv
• Kumpf, Robert; Shi, Chunlin; Larrieu, Antoine; Stø, Ida Myhrer; Butenko, Melinka Alonso & Péret, Benjamin [Vis alle 9 forfattere av denne artikkelen] (2013). Floral organ abscission peptide IDA and its HAE/HSL2 receptors control cell separation during lateral root emergence. Proceedings of the National Academy of Sciences of the United States of America (PNAS). ISSN 0027-8424. 110(13), s. 5235–5240. doi: 10.1073/pnas.1210835110. Fulltekst i vitenarkiv
• Shi, Chunlin; Stenvik, Grethe-Elisabeth; Vie, Ane Kjersti; Bones, Atle M.; Pautot, V & Proveniers, M [Vis alle 8 forfattere av denne artikkelen] (2011). Arabidopsis Class I KNOTTED-Like Homeobox Proteins Act Downstream in the IDA-HAE/HSL2 Floral Abscission Signaling Pathway. The Plant Cell. ISSN 1040-4651. 23(7), s. 2553–2567. doi: 10.1105/tpc.111.084608. Fulltekst i vitenarkiv

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• Butenko, Melinka Alonso; Shi, Chunlin & Aalen, Reidunn B. (2010). Class I KNOX proteins involved in floral abscission downstream in the IDA-HAE/HSL2 signaling pathway. Fulltekst i vitenarkiv

Se alle arbeider i NVA