Dirk Linke

Professor - Seksjon for genetikk og evolusjonsbiologi

English version of this page

E-post dirk.linke@ibv.uio.no

Telefon +47-22857654

Rom 3517

Brukernavn

Besøksadresse Blindernveien 31 None 0371 OSLO

Postadresse Postboks 1066 Blindern 0316 OSLO

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Academic Interests

My research group focusses on the structure and function of the bacterial cell surface. Currently, we work on three main topics:

Bacterial Adhesion: How do bacteria stick to surfaces, or in the case of pathogens, to host cells?
Membrane transport: how are adhesion molecules transported to the cell surface? And can this process be inhibited with novel antimicrobial substances?
Vaccines and Biomarkers: can we exploit bacterial surface proteins as vaccine candidates or as biomarkers, e.g. for improved clinical diagnostics?

Our work is typically comparative – we do not work on a single pathogenic “pet” organism, but instead compare related surface proteins from different species, studying their similarities and subtle differences. Our work is also interdisciplinary – we use methods from bioinformatics, structural biology and biophysics on top of classical methods from microbiology, molecular biology and biochemistry.

Teaching

MBV 3060 (Basic Microbiology)
MBV 4910 (iGEM Competition)
...

Higher education and employment history

since 2015 Full Professor, Department of Biosciences, UiO
since 2013 Associate Professor, Department of Biosciences, UiO
2010 Habilitation, Department of Biology, University of Tübingen
2007-2013 Project Leader, Max Planck Institute for Developmental Biology, Tübingen
2003-2007 PostDoc, Max Planck Institute for Developmental Biology, Tübingen
2002 PhD, Department of Chemistry, TU Berlin
1991-1997, undergraduate studies in Biochemistry, FU Berlin

Honoraria

2009 Young Academics Award from the German Society for Hygiene and Microbiology
2008 and 2009 two 1-year stipends from the Bill & Melinda Gates Foundation
...

Appointments

Advisory Board Member, Molecular Microbiology (Wiley)
Review Editor, Journal of Biological Chemistry
Review Editor, Frontiers in Microbiology

Chauhan, Nandini; Hatlem, Daniel; Rydmark, Marcella Orwick; Schneider, Kenneth; Flötenmeyer, Matthias; Van Rossum, Barth-J; Leo, Jack Christopher & Linke, Dirk (2019). Insights into the autotransport process of a trimeric autotransporter, Yersinia Adhesin A (YadA). Molecular Microbiology. ISSN 0950-382X. 111(3), s 844- 862 . doi: 10.1111/mmi.14195
Hatlem, Daniel; Trunk, Thomas; Linke, Dirk & Leo, Jack Christopher (2019). Catching a SPY: Using the SpyCatcher-SpyTag and Related Systems for Labeling and Localizing Bacterial Proteins. International Journal of Molecular Sciences. ISSN 1422-0067. 20(9) . doi: 10.3390/ijms20092129 Fulltekst i vitenarkiv. Vis sammendrag
The SpyCatcher-SpyTag system was developed seven years ago as a method for protein ligation. It is based on a modified domain from a Streptococcus pyogenes surface protein (SpyCatcher), which recognizes a cognate 13-amino-acid peptide (SpyTag). Upon recognition, the two form a covalent isopeptide bond between the side chains of a lysine in SpyCatcher and an aspartate in SpyTag. This technology has been used, among other applications, to create covalently stabilized multi-protein complexes, for modular vaccine production, and to label proteins (e.g., for microscopy). The SpyTag system is versatile as the tag is a short, unfolded peptide that can be genetically fused to exposed positions in target proteins; similarly, SpyCatcher can be fused to reporter proteins such as GFP, and to epitope or purification tags. Additionally, an orthogonal system called SnoopTag-SnoopCatcher has been developed from an S. pneumoniae pilin that can be combined with SpyCatcher-SpyTag to produce protein fusions with multiple components. Furthermore, tripartite applications have been produced from both systems allowing the fusion of two peptides by a separate, catalytically active protein unit, SpyLigase or SnoopLigase. Here, we review the current state of the SpyCatcher-SpyTag and related technologies, with a particular emphasis on their use in vaccine development and in determining outer membrane protein localization and topology of surface proteins in bacteria.
Meuskens, Ina; Saragliadis, Athanasios; Leo, Jack Christopher & Linke, Dirk (2019). Type V Secretion Systems: An Overview of Passenger Domain Functions. Frontiers in Microbiology. ISSN 1664-302X. 10 . doi: 10.3389/fmicb.2019.01163
Micenková, Lenka; Bosák, J; Kucera, J; Hrala, M; Dolejšová, T; Zdráhal, Z; Linke, Dirk; Fišer, F & Smajs, D (2019). Colicin Z, a structurally and functionally novel colicin type that selectively kills enteroinvasive Escherichia coli and Shigella strains. Scientific Reports. ISSN 2045-2322. 9(1) . doi: 10.1038/s41598-019-47488-8
Weidensdorfer, M; Ishikawa, M; Hori, Katsutoshi; Linke, Dirk; Djahanshiri, Bardya; Iruegas, R; Ebersberger, S; Riedel-Christ, S; Enders, G.; Leukert, L; Kraizcy, P; Rothweiler, F; Cinatl, J; Berger, Jürgen; Hipp, K; Kempf, Volkhard A.J. & Gottig, Stephan (2019). The Acinetobacter trimeric autotransporter adhesin Ata controls key virulence traits of Acinetobacter baumannii. Virulence. ISSN 2150-5594. 10, s 68- 81 . doi: 10.1080/21505594.2018.1558693
Leo, Jack Christopher & Linke, Dirk (2018). A unified model for BAM function that takes into account type Vc secretion and species differences in BAM composition. AIMS Microbiology. ISSN 2471-1888. 4(3), s 455- 468 . doi: 10.3934/microbiol.2018.3.455 Fulltekst i vitenarkiv. Vis sammendrag
Transmembrane proteins in the outer membrane of Gram-negative bacteria are almost exclusively β-barrels. They are inserted into the outer membrane by a conserved and essential protein complex called the BAM (for β-barrel assembly machinery). In this commentary, we summarize current research into the mechanism of this protein complex and how it relates to type V secretion. Type V secretion systems are autotransporters that all contain a β-barrel transmembrane domain inserted by BAM. In type Vc systems, this domain is a homotrimer. We argue that none of the current models are sufficient to explain BAM function particularly regarding type Vc secretion. We also find that current models based on the well-studied model system Escherichia coli mostly ignore the pronounced differences in BAM composition between different bacterial species. We propose a more holistic view on how all OMPs, including autotransporters, are incorporated into the lipid bilayer.
Wrobel, Agnieszka; Ottoni, Claudio; Leo, Jack Christopher & Linke, Dirk (2018). pYR4 From a Norwegian Isolate of Yersinia ruckeri Is a Putative Virulence Plasmid Encoding Both a Type IV Pilus and a Type IV Secretion System. Frontiers in Cellular and Infection Microbiology. ISSN 2235-2988. 8, s 1- 14 . doi: 10.3389/fcimb.2018.00373 Fulltekst i vitenarkiv. Vis sammendrag
Enteric redmouth disease caused by the pathogen Yersinia ruckeri is a significant problem for fish farming around the world. Despite its importance, only a few virulence factors of Y. ruckeri have been identified and studied in detail. Here, we report and analyze the complete DNA sequence of pYR4, a plasmid from a highly pathogenic Norwegian Y. ruckeri isolate, sequenced using PacBio SMRT technology. Like the well-known pYV plasmid of human pathogenic Yersiniae, pYR4 is a member of the IncFII family. Thirty-one percent of the pYR4 sequence is unique compared to other Y. ruckeri plasmids. The unique regions contain, among others genes, a large number of mobile genetic elements and two partitioning systems. The G+C content of pYR4 is higher than that of the Y. ruckeri NVH_3758 genome, indicating its relatively recent horizontal acquisition. pYR4, as well as the related plasmid pYR3, comprises operons that encode for type IV pili and for a conjugation system (tra). In contrast to other Yersinia plasmids, pYR4 cannot be cured at elevated temperatures. Our study highlights the power of PacBio sequencing technology for identifying mis-assembled segments of genomic sequences. Comparative analysis of pYR4 and other Y. ruckeri plasmids and genomes, which were sequenced by second and the third generation sequencing technologies, showed errors in second generation sequencing assemblies. Specifically, in the Y. ruckeri 150 and Y. ruckeri ATCC29473 genome assemblies, we mapped the entire pYR3 plasmid sequence. Placing plasmid sequences on the chromosome can result in erroneous biological conclusions. Thus, PacBio sequencing or similar long-read methods should always be preferred for de novo genome sequencing. As the tra operons of pYR3, although misplaced on the chromosome during the genome assembly process, were demonstrated to have an effect on virulence, and type IV pili are virulence factors in many bacteria, we suggest that pYR4 directly contributes to Y. ruckeri virulence.
Meuskens, Ina; Michalik, Marcin; Chauhan, Nandini; Linke, Dirk & Leo, Jack Christopher (2017). A New Strain Collection for Improved Expression of Outer Membrane Proteins. Frontiers in Cellular and Infection Microbiology. ISSN 2235-2988. 7 . doi: 10.3389/fcimb.2017.00464 Fulltekst i vitenarkiv. Vis sammendrag
Almost all integral membrane proteins found in the outer membranes of Gram-negative bacteria belong to the transmembrane β-barrel family. These proteins are not only important for nutrient uptake and homeostasis, but are also involved in such processes as adhesion, protein secretion, biofilm formation, and virulence. As surface exposed molecules, outer membrane β-barrel proteins are also potential drug and vaccine targets. High production levels of heterologously expressed proteins are desirable for biochemical and especially structural studies, but over-expression and subsequent purification of membrane proteins, including outer membrane proteins, can be challenging. Here, we present a set of deletion mutants derived from E. coli BL21(DE3) designed for the over-expression of recombinant outer membrane proteins. These strains harbor deletions of four genes encoding abundant β-barrel proteins in the outer membrane (OmpA, OmpC, OmpF, and LamB), both single and in all combinations of double, triple, and quadruple knock-outs. The sequences encoding these outer membrane proteins were deleted completely, leaving only a minimal scar sequence, thus preventing the possibility of genetic reversion. Expression tests in the quadruple mutant strain with four test proteins, including a small outer membrane β-barrel protein and variants thereof as well as two virulence-related autotransporters, showed significantly improved expression and better quality of the produced proteins over the parent strain. Differences in growth behavior and aggregation in the presence of high salt were observed, but these phenomena did not negatively influence the expression in the quadruple mutant strain when handled as we recommend. The strains produced in this study can be used for outer membrane protein production and purification, but are also uniquely useful for labeling experiments for biophysical measurements in the native membrane environment.
Michalik, Marcin; Rydmark, Marcella Orwick; Habeck, Michael; Alva, Vikram; Arnold, Thomas & Linke, Dirk (2017). An evolutionarily conserved glycine-tyrosine motif forms a folding core in outer membrane proteins. PLoS ONE. ISSN 1932-6203. 12(8) . doi: 10.1371/journal.pone.0182016 Fulltekst i vitenarkiv.
Mühlenkamp, Melanie C.; Hallstrom, Teresia; Autenrieth, Ingo B.; Bohn, Erwin; Linke, Dirk; Rinker, Janina; Riesbeck, Kristian; Singh, Birendra; Leo, Jack Christopher; Hammerschmidt, Sven; Zipfel, Peter F. & Schütz, Monika (2017). Vitronectin binds to a specific stretch within the head region of yersinia adhesin a and thereby modulates yersinia enterocolitica host interaction. Journal of Innate Immunity. ISSN 1662-811X. 9(1), s 33- 51 . doi: 10.1159/000449200 Fulltekst i vitenarkiv.
Wrobel, Agnieszka; Ottoni, Claudio; Leo, Jack Christopher; Gulla, Snorre & Linke, Dirk (2017). The repeat structure of two paralogous genes, Yersinia ruckeri invasin (yrInv) and a “Y. ruckeri invasin-like molecule”, (yrIlm) sheds light on the evolution of adhesive capacities of a fish pathogen.. Journal of Structural Biology. ISSN 1047-8477. 201(2), s 171- 183 . doi: 10.1016/j.jsb.2017.08.008
Curkić, I; Schütz, Monika; Oberhettinger, Philipp; Diard, P; Claassen, M; Linke, Dirk & Hardt, W (2016). Epitope-Tagged Autotransporters as Single-Cell Reporters for Gene Expression by a Salmonella Typhimurium wbaP Mutant. PLoS ONE. ISSN 1932-6203. 11(5) . doi: 10.1371/journal.pone.0154828 Fulltekst i vitenarkiv.
Fan, Enguo; Chauhan, Nandini; Udatha, D.B.R.K. Gupta; Leo, Jack Christopher & Linke, Dirk (2016). Type V secretion systems in bacteria, In Indira T. Kudva; Nancy A. Cornick; Paul J. Plummer; Qijing Zhang; Tracy L. Nicholson; John P. Bannantine & Bryan H. Bellaire (ed.), Virulence Mechanisms of Bacterial Pathogens. ASM International. ISBN 9781555819279. Chapter 11. s 305 - 335
Koiwai, Kotaro; Hartmann, Marcus; Linke, Dirk; Lupas, Andrei & Hori, Katsutoshi (2016). Structural basis for toughness and flexibility in the C-terminal passenger domain of an acinetobacter trimeric autotransporter adhesin. Journal of Biological Chemistry. ISSN 0021-9258. 291(8), s 3705- 3724 . doi: 10.1074/jbc.M115.701698 Fulltekst i vitenarkiv.
Leo, Jack Christopher; Oberhettinger, Philipp; Yoshimoto, Shogo; Udatha, D.B.R.K. Gupta; Morth, Jens Preben; Schutz, Monika; Hori, Katsutoshi & Linke, Dirk (2016). Secretion of the Intimin passenger domain is driven by protein folding. Journal of Biological Chemistry. ISSN 0021-9258. 291(38), s 20096- 20112 . doi: 10.1074/jbc.M116.731497 Fulltekst i vitenarkiv.
Michalik, Marcin; Djahanshiri, Bardya; Leo, Jack Christopher & Linke, Dirk (2016). Reverse Vaccinology: The Pathway from Genomes and Epitope Predictions to Tailored Recombinant Vaccines, In Sunil Thomas (ed.), Vaccine Design Methods and Protocols: Volume 1: Vaccines for Human Diseases. Springer. ISBN 978-1-4939-3385-3. Chapter 4. s 87 - 106
Pozzi, Roberta; Coles, Murray; Linke, Dirk; Kulik, Andreas; Nega, Mulugeta; Wohlleben, Wolfgang & Stegmann, Evi (2016). Distinct mechanisms contribute to immunity in the lantibiotic NAI-107 producer strain MicrobisporaATCC PTA-5024. Environmental Microbiology. ISSN 1462-2912. 18(1), s 118- 132 . doi: 10.1111/1462-2920.12892
Rydmark, Marcella Orwick; Arnold, Thomas & Linke, Dirk (2016). The use of detergents to purify membrane proteins. Current Protocols in Protein Science. ISSN 1934-3655. 2016, s 4.8.1- 4.8.35 . doi: 10.1002/0471140864.ps0408s84
Kretschmer, D; Rautenberg, M; Linke, Dirk & Peschel, A (2015). Peptide length and folding state govern the capacity of staphylococcal beta-type phenol-soluble modulins to activate human formyl-peptide receptors 1 or 2. Journal of Leukocyte Biology. ISSN 0741-5400. 97(4), s 689- 697 . doi: 10.1189/jlb.2A0514-275R
Leo, Jack Christopher; Oberhettinger, Philipp; Chaubey, Manish; Schütz, Monika; Kuhner, Daniel; Bertsche, Ute; Schwarz, Heinz; Gotz, Friedrich; Autenrieth, Ingo B. & Linke, Dirk (2015). The Intimin periplasmic domain mediates dimerisation and binding to peptidoglycan. Molecular Microbiology. ISSN 0950-382X. 95(1), s 80- 100 . doi: 10.1111/mmi.12840 Fulltekst i vitenarkiv.
Leo, Jack Christopher; Oberhettinger, Philipp; Schütz, Monika & Linke, Dirk (2015). The inverse autotransporter family: Intimin, invasin and related proteins.. International Journal of Medical Microbiology. ISSN 1438-4221. 305(2), s 276- 282 . doi: 10.1016/j.ijmm.2014.12.011

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Chauhan, Nandini; Hatlem, Daniel; Orwick-Rydmark, Marcella; Schneider, Kenneth; Linke, Dirk & Leo, Jack Christopher (2019). Using the SpyCatcher-SpyTag technology for topology mapping of outer membrane proteins in Gram-negative bacteria.
Chowdhury, Sounak; Happonen, Lotta Johanna; Linke, Dirk & Malmström, Johan (2019). QUANTITATIVE AFFINITY-PURIFICATION AND SURFACE-ADSORPTION MASS SPECTROMETRY TO MAP STREPTOCOCCUS PYOGENES AND HUMAN PROTEIN INTERACTION.
Hatlem, Daniel; Chauhan, Nandini; Orwick-Rydmark, Marcella; Schneider, Kenneth; Flötenmeyer, Matthias; Van Rossum, Barth-J; Leo, Jack Christopher & Linke, Dirk (2019). Insights into the autotransport of a trimeric autotransporter.
Kandanur, Sai Priya Sarma; Linke, Dirk & Leo, Jack Christopher (2019). Hairpin Formation and Passenger Secretion in the Inverse Autotransporter Intimin.
Leukert, L; Weidensdorfer, M; Djahanshiri, Bardya; Linke, Dirk; Kempf, Volkhard A.J. & Gottig, Stephan (2019). The Acinetobacter Trimeric Autotransporter Adhesin Ata Controls Key Virulence Traits of Acinetobacter baumannii.
Leva, Juan; Meuskens, Ina; Gibson, Timothy M.; Linke, Dirk & Millner, Paul (2019). Biosensors for pathogen detection.
Linke, Dirk (2019). Autotransporter Adhesins in the fish pathogen Yersinia ruckeri.
Linke, Dirk (2019). Insights into the autotransport process of a trimeric autotransporter, YadA.
Linke, Dirk & Saragliadis, Athanasios (2019). Bacterial adhesion to living and non-living surfaces.
Meuskens, Ina; Leva, Juan; Millner, Paul & Linke, Dirk (2019). Differential Adhesion of Trimeric Autotransporter Adhesins to Extracellular Matrix Proteins.
Meuskens, Ina & Linke, Dirk (2019). Binding of Yersinia Ahesin A to ECM molecules: a biosensor approach.
Orwick-Rydmark, Marcella; Chauhan, Nandini; Van Rossum, Barth-J & Linke, Dirk (2019). Towards the structure of the YadA anchor domain in the native membrane.
Orwick-Rydmark, Marcella; Van Rossum, Barth-J; Oschkinat, Hartmut & Linke, Dirk (2019). Towards the in situ structure of the Yersinia Adhesin A membrane anchor domain.
Røsjø, Bjarne; Orwick-Rydmark, Marcella; Linke, Dirk; Leo, Jack Christopher & Hatlem, Daniel (2019, 13. mai). Ny antibiotika-idé: Vil hindre bakteriene i å feste seg. [Internett]. TITAN.NO.
Saragliadis, Athanasios & Linke, Dirk (2019). Assay development for the discovery of small-molecule inhibitors of YadA adhesion to collagen.
Saragliadis, Athanasios & Linke, Dirk (2019). Screening for small-molecule inhibitors of YadA adhesion to collagen.
Thibau, Arno; Vaca, Diana; Linke, Dirk; Ballhorn, Wibke & Kempf, Volkhard A.J. (2019). Analysing the Fibronectin Binding Domain of the Bartonella henselae Adhesin A.
Tietgen, M; Weidensdorfer, M; Djahanshiri, Bardya; Linke, Dirk; Kempf, Volkhard A.J. & Gottig, Stephan (2019). Virulence of Acinetobacter baumannii depends on the Acinetobacter trimeric autotransporter adhesin Ata.
Balusek, Curtis; Linke, Dirk & Gumbart, JC (2018). The effect of beta-barrel geometry on ion conduction through Escherichia coli OmpX.
Hatlem, Daniel & Linke, Dirk (2018). Insights into the autotransporter process of a trimeric autotransporter, Yersinia Adhesin A (YadA).
Joudeh, Nadeem; Leo, Jack Christopher; Torgeman, Eric & Linke, Dirk (2018). Mechanism of Palladium and Gold uptake and reduction in E. coli.
Khalil, Hawzeen; Øgaard, Jonas; Linke, Dirk & Leo, Jack Christopher (2018). Co-aggregation properties of trimeric autotransporter adhesins.
Leo, Jack Christopher; Schütz, Monika & Linke, Dirk (2018). Type Ve Secretion in Enterobacteria.
Linke, Dirk (2018). Structural Biology of Membrane Proteins: Expression Tricks, and to Solubilize or Not to Solubilize.
Linke, Dirk (2018). Trimeric Autotransporter Adhesins and routes to their inhibition.
Linke, Dirk & Meuskens, Ina (2018). Adhesion of Yersinia Adhesin A to Collagen.
Linke, Dirk & Saragliadis, Athanasios (2018). Trimeric Autotransporter Adhesins - Understanding adhesive function and screening for inhibitors.
Linke, Dirk & Saragliadis, Athanasios (2018). Trimeric Autotransporter Adhesins - understanding adhesive function and screening for inhibitors.
Wrobel, Agnieszka; Ottoni, Claudio; Leo, Jack Christopher & Linke, Dirk (2018). The repeat structure of two paralogous genes, Yersinia ruckeri invasin (yrInv) and a “Y. ruckeri invasin-like molecule”, (yrIlm) sheds light on the evolution of adhesive capacities of a fish pathogen.
Wrobel, Agnieszka; Saragliadis, Athanasios; Leo, Jack Christopher; Ottoni, Claudio & Linke, Dirk (2018). Type Ve autotransporters in Yersinia ruckeri.
Økstad, Ole Andreas Løchen & Linke, Dirk (2018). Hvor farlig er antibiotikaresistens?. Ingeniørenes Stemme. Vinter 2018, s 37- 37
Hatlem, Daniel; Leo, Jack Christopher; Saragliadis, Athanasios; Krengel, Ute & Linke, Dirk (2017). Bacterial adhesion: Characterization of trimeric autotransporter binding.
Joudeh, Nadeem; Leo, Jack Christopher; Torgeman, Eric; Mikheenko, Pavlo & Linke, Dirk (2017). Mechanism of Palladium and Gold uptake and transport in E. coli.
Joudeh, Nadeem & Linke, Dirk (2017). Mechanism of palladium and gold uptake and transport in E. coli.
Leo, Jack Christopher & Linke, Dirk (2017). Inverse autotransport: the mechanism of type Ve secretion..
Leo, Jack Christopher; Oberhettinger, Philipp; Schütz, Monika & Linke, Dirk (2017). Inverse autotransport: the mechanism of type Ve secretion.
Linke, Dirk (2017). Do we have to purify membrane proteins? Solid-state NMR in the native membrane.
Linke, Dirk (2017). Trimeric Autotransporter Evolution.
Linke, Dirk (2017). Trimeric Autotrasnporter Adhesins: Stucture, Function, and Evolution.
Linke, Dirk (2017). Trimeric autotransporter adhesins (TAAs) and routes to their inhibition.
Linke, Dirk (2017). Type Ve secretion in Gram-negative Bacteria.
Liskiewicz, Krystyna; Wrobel, Agnieszka; Leo, Jack Christopher & Linke, Dirk (2017). Identification of potential adhesin receptors from Yersinia ruckeri.
Michalik, Marcin; Orwick-Rydmark, Marcella; Arnold, Thomas; Habeck, Michael & Linke, Dirk (2017). An evolutionarily conserved motif forms a folding core in Outer membrane proteins.
Orwick-Rydmark, Marcella; Chauhan, Nandini; Shahid, Shakeel & Linke, Dirk (2017). Solid-state NMR studies on Yersinia YadA: from autotransport to drug screening.
Saragliadis, Athanasios & Linke, Dirk (2017). STRUCTURE-FUNCTION RELATIONSHIP IN BACTERIAL ADHESINS.
Schneider, Kenneth; Haugen, Håvard Jostein & Linke, Dirk (2017). Assay Development: Adhesin Interaction with Implant Materials.
Torgeman, Eric; Linke, Dirk & Leo, Jack Christopher (2017). Biosynthesis of gold and palladium nanoparticles via bacteria.
Weidensdorfer, M; Linke, Dirk; Kempf, Volkhard A.J. & Gottig, Stephan (2017). Establishing of a dynamic human ex vivo infection model for analyzing endothelial adherence of Acinetobacter baumannii.
Wrobel, Agnieszka; Leo, Jack Christopher; Ottoni, Claudio; Gulla, Snorre & Linke, Dirk (2017). Comparative structure-function analysis of adhesins from different Yersinia ruckeri strains.
Wrobel, Agnieszka & Linke, Dirk (2017). The repeat structure of two paralogous invasin genes from Yersinia ruckeri sheds light on the evolution of adhesive capacities of a Fish pathogen.
Wrobel, Agnieszka; Ottoni, Claudio; Leo, Jack Christopher & Linke, Dirk (2017). IDENTIFICATION AND CHARACTERIZATION OF INVERSE AUTOTRANSPORTERS FROM A FISH PATHOGEN, YERSINIA RUCKERI.
Wrobel, Agnieszka; Ottoni, Claudio; Leo, Jack Christopher & Linke, Dirk (2017). IDENTIFICATION AND CHARACTERIZATION OF INVERSE AUTOTRANSPORTERS FROM A FISH PATHOGEN, YERSINIA RUCKERI..
Hatlem, Daniel; Kempf, Volkhard A.J.; Krengel, Ute & Linke, Dirk (2016). Bacterial adhesion: Characterization of trimeric autotransporter binding.
Leo, Jack Christopher & Linke, Dirk (2016). Inverse autotransport: surface export of Intimin and other type Ve secretion systems.
Leo, Jack Christopher & Linke, Dirk (2016). Secretion of the Intimin Passenger Domain Is Driven by Protein Folding..
Leo, Jack Christopher & Linke, Dirk (2016). Type Ve secretion - inverse autotransport.
Leo, Jack Christopher; Oberhettinger, Philipp; Schütz, Monika; Hori, Katsutoshi; Yoshimoto, Shogo & Linke, Dirk (2016). The secretion of the Intimin passenger is driven by protein folding.
Leo, Jack Christopher; Yoshimoto, Shogo; Oberhettinger, Philipp; Schütz, Monika; Hori, Katsutoshi & Linke, Dirk (2016). The secretion of the Intimin passenger domain is driven by protein folding..
Linke, Dirk (2016, 10. februar). NRK Forbrukerinspektørene. [TV]. NRK. Vis sammendrag
Episodebeskrivelse Hva er egentlig forskjellen på en dyr og en billig dress, og kan fremtidens plagg fortelle oss når vi blir syke? Forbrukerinspektørene ser nærmere på klær og hva de er laget av. Bambus for eksempel. Er det virkelig det supermaterialet som mange produsenter gir inntrykk av at det er? (Program made partially at IBV with Dirk Linke, Bård Mathiesen, Agniesazka Wrobel)
Linke, Dirk (2016). Solid-State NMR Studies of Yersinia Adhesin A: From Autotransport Mechansim to Drug Screening.
Linke, Dirk (2016). Structre, Function and Evolution of Trimeric Autotransporter Adhesins.
Linke, Dirk (2016). Structure and dynamics of the Translocation Domain of an Autotransporter by Nuclear Magnetic Resonance and Molecular Dynamics Simulations.
Linke, Dirk (2016). Structure, Function and Evolution of Trimeric Autotransporter Adhesins.
Michalik, Marcin & Linke, Dirk (2016). Identification and evaluation of peptides for active and passive immunization against Pseudomonas aeruginosa.
Michalik, Marcin & Linke, Dirk (2016). Identification and evaluation of peptides for immunization against Pseudomonas aeruginosa.
Orwick-Rydmark, Marcella; Arnold, Thomas & Linke, Dirk (2016). The Use of Detergents to Purify Membrane Proteins, In JE Coligan; BM Dunn; DW Speicher & PT Wignfield (ed.), Current Protocols in Protein Science. John Wiley & Sons. ISBN 978-0-471-11184-9. Unit 4.8. s 4.8.1. - 4.8.35
Rydmark, Marcella Orwick; Chauhan, Nandini & Linke, Dirk (2016). Structure and Dynamics of The Yersinia Adhesin A Membrane-Anchor Domain by NMR.
Schneider, Kenneth; Saragliadis, Athanasios; Haugen, Håvard Jostein & Linke, Dirk (2016). Aggregatibacter Adhesins and their interaction with implant materials.
Wrobel, Agnieszka & Linke, Dirk (2016). Zebrafish as a model system for studying Yersinia ruckeri infections.
Wrobel, Agnieszka & Linke, Dirk (2016). Zebrafish embryos as a model system for studying Yersinia ruckeri infection.
Chauhan, Nandini & Linke, Dirk (2015). Correction to the paper: "Purification of the YadA membrane anchor for secondary structure analysis and crystallization". International Journal of Biological Macromolecules. ISSN 0141-8130. 81, s 599 . doi: 10.1016/j.ijbiomac.2015.08.052
Chauhan, Nandini & Linke, Dirk (2015). Insights into the autotransport mechanism of the trimeric autotransporter YadA.
Chauhan, Nandini; Shahid, Shakeel & Linke, Dirk (2015). Insights into the autotransport mechanism of trimeric autotransporter YadA.
Hatlem, Daniel; Saragliadis, Athanasios; Leo, Jack Christopher; Shahid, Shakeel; van Rossum, Barth & Linke, Dirk (2015). TAAs: fibrous adhesins on the bacterial cell surface.
Leo, Jack Christopher & Linke, Dirk (2015, 01. oktober). A Talk with Dr. Dirk Linke and Dr. Jack Leo. [Tidsskrift]. Micrographia Today Vol 2 Issue 3 pp 43-46.
Leo, Jack Christopher; Oberhettinger, Philipp; Chaubey, Manish; Schütz, Monika; Coles, Murray & Linke, Dirk (2015). The Intimin periplasmic domain mediates dimerisation and binding to peptidoglycan.
Leo, Jack Christopher; Oberhettinger, Philipp; Chaubey, Manish; Schütz, Monika; Kuhner, Daniel; Bertsche, Ute; Schwarz, Heinz; Gotz, Friedrich; Autenrieth, Ingo B.; Coles, Murray & Linke, Dirk (2015). The Intimin periplasmic domain mediates binding to peptidoglycan.
Leo, Jack Christopher; Oberhettinger, Philipp & Linke, Dirk (2015). Assessing the Outer Membrane Insertion and Folding of Multimeric Transmembrane β-Barrel Proteins, In Susan Buchanan & Nicholas Noinaj (ed.), The BAM Complex: Methods and Protocols. Springer. ISBN 978-1-4939-2870-5. Chapter 12. s 157 - 168
Leo, Jack Christopher; Schneider, Kenneth & Linke, Dirk (2015). Autotransporters as mediators of bacterial adhesion.

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Publisert 16. okt. 2013 09:29 - Sist endret 29. jan. 2015 11:53

Dirk Linke

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Phone, fax, e-mail