Eili Tranheim Kase

• Stevanovic, Stanislava; Dalmao-Fernandez, Andrea; Mohamed, Derya; Nyman, Tuula Anneli; Kostovski, Emil & Iversen, Per Ole [Vis alle 11 forfattere av denne artikkelen] (2024). Time-dependent reduction in oxidative capacity among cultured myotubes from spinal cord injured individuals. Acta Physiologica. ISSN 1748-1708. doi: 10.1111/apha.14156.
• Krapf, Solveig; Lund, Jenny; Bakke, Hege Gilbø; Nyman, Tuula Anneli; Bartesaghi, Stefano & Peng, Xiao-Rong [Vis alle 9 forfattere av denne artikkelen] (2023). SENP2 knockdown in human adipocytes reduces glucose metabolism and lipid accumulation, while increases lipid oxidation . Metabolism Open. ISSN 2589-9368. 18. doi: 10.1016/j.metop.2023.100234. Fulltekst i vitenarkiv
• Dalmao-Fernandez, Andrea; Aizenshtadt, Aleksandra; Bakke, Hege Gilbø; Krauss, Stefan Johannes Karl; Rustan, Arild Christian & Thoresen, G. Hege [Vis alle 7 forfattere av denne artikkelen] (2023). Development of three-dimensional primary human myospheres as culture model of skeletal muscle cells for metabolic studies. Frontiers in Bioengineering and Biotechnology. ISSN 2296-4185. 11, s. 1–13. doi: 10.3389/fbioe.2023.1130693. Fulltekst i vitenarkiv
• Krapf, Solveig; Lund, Jenny; Saqib, Awais Ur Rehman; Bakke, Hege Gilbø; Rustan, Arild Christian & Thoresen, G. Hege [Vis alle 7 forfattere av denne artikkelen] (2022). Pancreatic Cancer Cell-Conditioned, Human-Derived Primary Myotubes Display Increased Leucine Turnover, Increased Lipid Accumulation, and Reduced Glucose Uptake. Metabolites. ISSN 2218-1989. 12(11). doi: 10.3390/metabo12111095. Fulltekst i vitenarkiv
• Katare, Parmeshwar Bajirao; Dalmao Fernandez, Andrea; Mengeste, Abel Mulu; Hamarsland, Håvard; Ellefsen, Stian & Bakke, Hege Gilbø [Vis alle 9 forfattere av denne artikkelen] (2022). Energy metabolism in skeletal muscle cells from donors with different body mass index. Frontiers in Physiology. ISSN 1664-042X. 13. doi: 10.3389/fphys.2022.982842.
• Mengeste, Abel Mulu; Nikolic, Natasa; Dalmao Fernandez, Andrea; Feng, Yuan Zeng; Nyman, Tuula Anneli & Kersten, Sander [Vis alle 11 forfattere av denne artikkelen] (2022). Insight Into the Metabolic Adaptations of Electrically Pulse-Stimulated Human Myotubes Using Global Analysis of the Transcriptome and Proteome. Frontiers in Physiology. ISSN 1664-042X. 13. doi: 10.3389/fphys.2022.928195. Vis sammendrag

  Electrical pulse stimulation (EPS) has proven to be a useful tool to interrogate cell-specific responses to muscle contraction. In the present study, we aimed to uncover networks of signaling pathways and regulatory molecules responsible for the metabolic effects of exercise in human skeletal muscle cells exposed to chronic EPS. Differentiated myotubes from young male subjects were exposed to EPS protocol 1 (i.e. 2 ms, 10 V, and 0.1 Hz for 24 h), whereas myotubes from middle-aged women and men were exposed to protocol 2 (i.e. 2 ms, 30 V, and 1 Hz for 48 h). Fuel handling as well as the transcriptome, cellular proteome, and secreted proteins of EPS-treated myotubes from young male subjects were analyzed using a combination of high-throughput RNA sequencing, high-resolution liquid chromatography-tandem mass spectrometry, oxidation assay, and immunoblotting. The data showed that oxidative metabolism was enhanced in EPS-exposed myotubes from young male subjects. Moreover, a total of 81 differentially regulated proteins and 952 differentially expressed genes (DEGs) were observed in these cells after EPS protocol 1. We also found 61 overlapping genes while comparing the DEGs to mRNA expression in myotubes from the middle-aged group exposed to protocol 2, assessed by microarray. Gene ontology (GO) analysis indicated that significantly regulated proteins and genes were enriched in biological processes related to glycolytic pathways, positive regulation of fatty acid oxidation, and oxidative phosphorylation, as well as muscle contraction, autophagy/mitophagy, and oxidative stress. Additionally, proteomic identification of secreted proteins revealed extracellular levels of 137 proteins were changed in myotubes from young male subjects exposed to EPS protocol 1. Selected putative myokines were measured using ELISA or multiplex assay to validate the results. Collectively, our data provides new insight into the transcriptome, proteome and secreted proteins alterations following in vitro exercise and is a valuable resource for understanding the molecular mechanisms and regulatory molecules mediating the beneficial metabolic effects of exercise.

• Mengeste, Abel Mulu; Katare, Parmeshwar Bajirao; Dalmao Fernandez, Andrea; Lund, Jenny; Bakke, Hege Gilbø & Baker, David [Vis alle 11 forfattere av denne artikkelen] (2022). Knockdown of sarcolipin (SLN) impairs substrate utilization in human skeletal muscle cells. Molecular Biology Reports. ISSN 0301-4851. s. 6005–6017. doi: 10.1007/s11033-022-07387-0. Fulltekst i vitenarkiv
• Skagen, Christine; Nyman, Tuula Anneli; Xiao-Rong, Peng; O'Mahony, Sven; Kase, Eili Tranheim & Rustan, Arild Christian [Vis alle 7 forfattere av denne artikkelen] (2021). Chronic treatment with terbutaline increases glucose and oleic acid oxidation and protein synthesis in cultured human myotubes. Current Research in Pharmacology and Drug Discovery (CRPHAR). ISSN 2590-2571. 2, s. 1–11. doi: 10.1016/j.crphar.2021.100039.
• Mengeste, Abel Mulu; Lund, Jenny; Katare, Parmeshwar Bajirao; Ghobadi, Roya; Bakke, Hege Gilbø & Lunde, Per Kristian [Vis alle 13 forfattere av denne artikkelen] (2021). The small molecule SERCA activator CDN1163 increases energy metabolism in human skeletal muscle cells. Current Research in Pharmacology and Drug Discovery (CRPHAR). ISSN 2590-2571. 2, s. 1–12. doi: 10.1016/j.crphar.2021.100060.
• Lund, Jenny; Krapf, Solveig Astrid; Sistek, Medina; Bakke, Hege Gilbø; Bartesagi, Stefano & Xiao-Rong, Peng [Vis alle 9 forfattere av denne artikkelen] (2021). SENP2 is vital for optimal insulin signaling and insulin-stimulated glycogen synthesis in human skeletal muscle cells. Current Research in Pharmacology and Drug Discovery (CRPHAR). ISSN 2590-2571. 2, s. 1–10. doi: 10.1016/j.crphar.2021.100061.
• Krapf, Solveig Astrid; Schjølberg, Tiril; Asoawe, Lucia; Honkanen, Susanna Kyllikki; Kase, Eili Tranheim & Thoresen, G. Hege [Vis alle 7 forfattere av denne artikkelen] (2021). Novel methods for cold exposure of skeletal muscle in vivo and in vitro show temperature-dependent myokine production. Journal of Thermal Biology. ISSN 0306-4565. 98. doi: 10.1016/j.jtherbio.2021.102930. Fulltekst i vitenarkiv Vis sammendrag

  Proteins secreted from skeletal muscle serving a signalling role have been termed myokines. Many of the myokines are exercise factors, produced and released in response to muscle activity. Cold exposures affecting muscle may occur in recreational, occupational and therapeutic settings. Whether muscle temperature independently affects myokine profile, is still to be elucidated. We hypothesized that manipulating muscle temperature by means of external cooling would change myokine production and release. In the present study we have established new models for cold exposure of muscle in vivo and in vitro where rat hind limb or cultured human myotubes were cooled to 18 °C. After a recovery period, muscle tissue, cells and culture media were harvested for further analysis by qPCR and immunoassays. Expression of several myokine genes were significantly increased after cold exposure in both models: in rat muscle, mRNA levels of CCL2 (p = 0.04), VEGFA (p = 0.02), CXCL1 (p = 0.02) and RBM3 (p = 0.02) increased while mRNA levels of IL-6 (p = 0.03) were decreased; in human myotubes, mRNA levels of IL6 (p = 0.01), CXCL8 (p = 0.04), VEGFA (p = 0.03) and CXCL1 (p < 0.01) were significantly increased, as well as intracellular protein levels of IL-8 (CXCL8 gene product; p < 0.01). The corresponding effect on myokine secretion was not observed, on the contrary, IL-8 (p = 0.02) and VEGF (VEGFA gene product) p < 0.01) concentrations in culture media were reduced after cold exposure in vitro. In conclusion, cold exposure of muscle in vivo and in vitro had an effect on the production and release of several known exercise-related myokines. Myokine expression at the level of mRNA and protein was increased by cold exposure, whereas secretion tended to be decreased.

• Krapf, Solveig Astrid; Lund, Jenny; Lundkvist, Malin; Grebstad Dale, Marianne; Nyman, Tuula Anneli & Thoresen, G. Hege [Vis alle 7 forfattere av denne artikkelen] (2020). Pancreatic cancer cells show lower oleic acid oxidation and their conditioned medium inhibits oleic acid oxidation in human myotubes. Pancreatology (Print). ISSN 1424-3903. 20(4), s. 676–682. doi: 10.1016/j.pan.2020.04.014.
• Løvsletten, Nils Gunnar; Vu, Helene Loang Ngo; Skagen, Christine; Lund, Jenny; Kase, Eili Tranheim & Thoresen, G. Hege [Vis alle 8 forfattere av denne artikkelen] (2020). Treatment of human skeletal muscle cells with inhibitors of diacylglycerol acyltransferases 1 and 2 to explore isozyme-specific roles on lipid metabolism. Scientific Reports. ISSN 2045-2322. 10:238, s. 1–13. doi: 10.1038/s41598-019-57157-5. Fulltekst i vitenarkiv
• Løvsletten, Nils Gunnar; Bakke, Siril Skaret; Kase, Eili Tranheim; Ouwens, D. Margriet; Thoresen, G. Hege & Rustan, Arild (2018). Increased triacylglycerol - Fatty acid substrate cycling in human skeletal muscle cells exposed to eicosapentaenoic acid. PLOS ONE. ISSN 1932-6203. 13:e0208048(11), s. 1–15. doi: 10.1371/journal.pone.0208048. Fulltekst i vitenarkiv
• Lund, Jenny; Helle, Siw A; Li, Yuchuan; Løvsletten, Nils Gunnar; Stadheim, Hans Kristian & Jensen, Jørgen [Vis alle 9 forfattere av denne artikkelen] (2018). Higher lipid turnover and oxidation in cultured human myotubes from athletic versus sedentary young male subjects. Scientific Reports. ISSN 2045-2322. 8(1). doi: 10.1038/s41598-018-35715-7. Fulltekst i vitenarkiv Vis sammendrag

  In this study we compared fatty acid (FA) metabolism in myotubes established from athletic and sedentary young subjects. Six healthy sedentary (maximal oxygen uptake (VO2max)≤ 46 ml/kg/min) and six healthy athletic (VO2max> 60 ml/kg/min) young men were included. Myoblasts were cultured and diferentiated to myotubes from satellite cells isolated from biopsy of musculus vastus lateralis. FA metabolism was studied in myotubes using [14C]oleic acid. Lipid distribution was assessed by thin layer chromatography, and FA accumulation, lipolysis and re-esterifcation were measured by scintillation proximity assay.Gene and protein expressions were studied. Myotubes from athletic subjects showed lower FA accumulation, lower incorporation of FA into total lipids, triacylglycerol (TAG), diacylglycerol and cholesteryl ester, higherTAG-related lipolysis and re-esterifcation, and higher complete oxidation and incomplete β-oxidation of FA compared to myotubes from sedentary subjects. mRNA expression of the mitochondrial electron transport chain complex III gene UQCRB was higher in cells from athletic compared to sedentary. Myotubes established from athletic subjects have higher lipid turnover and oxidation compared to myotubes from sedentary subjects.Our fndings suggestthat cultured myotubes retain some of the phenotypic traits of their donors.

• Lund, Jenny; Tangen, Daniel S.; Wiig, Håvard; Stadheim, Hans Kristian; Siw, Anette Helle & Birk, Jesper B. [Vis alle 12 forfattere av denne artikkelen] (2017). Glucose metabolism and metabolic flexibility in cultured skeletal muscle cells is related to exercise status in young male subjects. Archives of Physiology and Biochemistry. ISSN 1381-3455. 124(2), s. 119–130. doi: 10.1080/13813455.2017.1369547. Fulltekst i vitenarkiv
• Feng, Yuan Zeng; Lund, Jenny; Li, Yuchuan; Knabenes, Irlin; Bakke, Siril Skaret & Kase, Eili Tranheim [Vis alle 11 forfattere av denne artikkelen] (2017). Loss of perilipin 2 in cultured myotubes enhances lipolysis and redirects the metabolic energy balance from glucose oxidation towards fatty acid oxidation. Journal of Lipid Research. ISSN 0022-2275. 58(11), s. 2147–2161. doi: 10.1194/jlr.M079764. Fulltekst i vitenarkiv
• Ho, Giang Thanh Thi; Kase, Eili Tranheim; Wangensteen, Helle & Barsett, Hilde (2017). Phenolic Elderberry Extracts, Anthocyanins, Procyanidins, and Metabolites Influence Glucose and Fatty Acid Uptake in Human Skeletal Muscle Cells. Journal of Agricultural and Food Chemistry. ISSN 0021-8561. 65(13), s. 2677–2685. doi: 10.1021/acs.jafc.6b05582.

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• Thoresen, G. Hege; Simonsen, terje; Christensen, Hege; Johansen, Harald Thidemann; Kase, Eili Tranheim & Robertsen, Ida [Vis alle 9 forfattere av denne artikkelen] (2021). Basal farmakologi. Fagbokforlaget. ISBN 9788245033557. 405 s.
• Thoresen, G. Hege; Solberg, Rigmor; Johansen, Harald Thidemann; Kase, Eili Tranheim; Rustan, Arild Christian & Christensen, Hege [Vis alle 7 forfattere av denne artikkelen] (2020). Illustrert farmakologi. Fagbokforlaget. ISBN 9788245033564. 392 s.

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